CD161 is a marker of all human IL-17-producing T-cell subsets and is induced by RORC.

Eur J Immunol 2010 Aug;40(8):2174-81

Centre of Excellence DENOThe, University of Florence, Italy.

We have previously shown that human Th17 lymphocytes are characterized by the selective expression of IL-23 receptor (IL-23R), CCR6, CD161, and the transcription factor retinoic acid-related orphan receptor C (RORC), and originate from a CD161(+)CD4(+) naïve T-cell precursor in response to the combined activity of IL-1β and IL-23. We show here that not only CD4(+)TCRαβ(+), but also CD8(+)TCRαβ(+), CD4(-)CD8(-) TCRαβ(+), and CD4(-)CD8(-) TCRγδ(+) circulating lymphocytes that produce IL-17 express the distinctive marker CD161 on their surface. In addition, we demonstrate that CD161 expression identifies CD8(+) and CD4(-)CD8(-) umbilical cord blood T cells that already express RORC and IL-23R mRNA and that can be induced to differentiate into IL-17-producing cells in the presence of IL-1β and IL-23. Finally, we provide evidence that umbilical cord blood naïve CD4(+)CD161(-) T cells, upon lentivirus-mediated transduction with RORC2 can acquire the ability to express IL-23R, IL-1RI, and CD161, as well as to produce IL-17. Taken together, these data allow to conclude that T-cell subsets able to produce IL-17, as well as precursors of IL-17-producing T cells, exhibit surface expression of CD161, and that this feature is at least in part RORC2-dependent.

Download full-text PDF

Source
http://dx.doi.org/10.1002/eji.200940257DOI Listing
August 2010
8 Reads

Publication Analysis

Top Keywords

produce il-17
12
il-1β il-23
8
il-17-producing cells
8
t-cell subsets
8
cord blood
8
umbilical cord
8
cd161
6
cd4-cd8- tcrαβ+
4
data allow
4
cd8+tcrαβ+ cd4-cd8-
4
mrna induced
4
tcrγδ+ circulating
4
il-17 data
4
cd4-cd8- tcrγδ+
4
induced differentiate
4
tcrαβ+ cd4-cd8-
4
allow conclude
4
response combined
4
subsets produce
4
precursor response
4

Altmetric Statistics

References

(Supplied by CrossRef)
Two types of murine helper T cell clones. I. Definition according to profiles of lymphokine activities and secreted proteins
Mosmann et al.
J. Immunol. 1986
Human TH1 and TH2 subsets: doubt no more
Romagnani et al.
Immunol. Today 1991
Expanding the effector CD4 T-cell repertoire: the Th17 lineage
Harrington et al.
Curr. Opin. Immunol. 2006
Th17 cells in immunity and autoimmunity
Oukka et al.
Ann. Rheum. Dis. (sUPPL3:iii) 2008

Similar Publications