Oocyte-type linker histone B4 is required for transdifferentiation of somatic cells in vivo.

FASEB J 2010 Sep 11;24(9):3462-7. Epub 2010 May 11.

Department of Biology, University of Dayton, Dayton, OH 45469-2320, USA.

The ability to reprogram in vivo a somatic cell after differentiation is quite limited. One of the most impressive examples of such a process is transdifferentiation of pigmented epithelial cells (PECs) to lens cells during lens regeneration in newts. However, very little is known of the molecular events that allow newt cells to transdifferentiate. Histone B4 is an oocyte-type linker histone that replaces the somatic-type linker histone H1 during reprogramming mediated by somatic cell nuclear transfer (SCNT). We found that B4 is expressed and required during transdifferentiation of PECs. Knocking down of B4 decreased proliferation and increased apoptosis, which resulted in considerable smaller lens. Furthermore, B4 knockdown altered gene expression of key genes of lens differentiation and nearly abolished expression of gamma-crystallin. These data are the first to show expression of oocyte-type linker histone in somatic cells and its requirement in newt lens transdifferentiation and suggest that transdifferentiation in newts might share common strategies with reprogramming after SCNT.

Download full-text PDF

Source
http://www.fasebj.org/doi/10.1096/fj.10-159285
Publisher Site
http://dx.doi.org/10.1096/fj.10-159285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923362PMC
September 2010
29 Reads

Publication Analysis

Top Keywords

linker histone
16
oocyte-type linker
12
somatic cell
8
required transdifferentiation
8
somatic cells
8
lens
5
histone
5
transdifferentiation
5
cells
5
histone oocyte-type
4
transdifferentiate histone
4
key genes
4
expression key
4
somatic-type linker
4
replaces somatic-type
4
cells transdifferentiate
4
histone replaces
4
lens differentiation
4
molecular events
4
abolished expression
4

References

(Supplied by CrossRef)
A mammalian oocyte‐specific linker histone gene H1oo: homology with the genes for the oocyte‐specific cleavage stage histone (cs‐H1) of sea urchin and the B4/H1M histone of the frog
Tanaka M. et al.
Development 2001

Similar Publications