Data storage based on photochromic and photoconvertible fluorescent proteins.

Authors:
Dr. Virgile Adam, PhD
Dr. Virgile Adam, PhD
CNRS / Institute for Structural Biology (IBS)
Research scientist
single molecule fluorescence microscopy, fluorescent protein engineering, X-ray crystallography
Grenoble | France

J Biotechnol 2010 Sep 21;149(4):289-98. Epub 2010 Apr 21.

Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium.

The recent discovery of photoconvertible and photoswitchable fluorescent proteins (PCFPs and RSFPs, respectively) that can undergo photoinduced changes of their absorption/emission spectra opened new research possibilities in subdiffraction microscopy and optical data storage. Here we demonstrate the proof-of-principle for read only and rewritable data storage both in 2D and 3D, using PCFPs and RSFPs. The irreversible burning of information was achieved by photoconverting from green to red defined areas in a layer of the PCFP Kaede. Data were also written and erased several times in layers of the photochromic fluorescent protein Dronpa. Using IrisFP, which combines the properties of PCFPs and RSFPs, we performed the first encoding of data in four colours using only one type of fluorescent protein. Finally, three-dimensional optical data storage was demonstrated using three mutants of EosFP (d1EosFP, mEosFP and IrisFP) in their crystalline form. Two-photon excitation allowed the precise addressing of regions of interest (ROIs) within the three-dimensional crystalline matrix without excitation of out-of-focus optical planes. Hence, this contribution highlights several data storage schemes based on the remarkable properties of PCFPs/RSFPs.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbiotec.2010.04.001DOI Listing
September 2010
8 Reads
5 Citations
2.871 Impact Factor

Publication Analysis

Top Keywords

data storage
20
pcfps rsfps
12
optical data
8
fluorescent proteins
8
fluorescent protein
8
data
7
properties pcfps
4
encoding data
4
rsfps performed
4
performed encoding
4
data colours
4
three-dimensional optical
4
storage demonstrated
4
finally three-dimensional
4
protein finally
4
colours type
4
type fluorescent
4
combines properties
4
protein dronpa
4
pcfp kaede
4

Similar Publications