Multicenter comparison of the ISO standard 20776-1 and the serial 2-fold dilution procedures to dilute hydrophilic and hydrophobic antifungal agents for susceptibility testing.

J Clin Microbiol 2010 May 10;48(5):1918-20. Epub 2010 Mar 10.

Servicio de MicologĂ­a, Centro Nacional de MicrobiologĂ­a, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km. 2, 28220 Majadahonda, Madrid, Spain.

A multicenter study was conducted to assess the accuracy of the ISO standard 20776-1 and the serial 2-fold dilution procedures for antifungal susceptibility testing. Fluconazole trays can be accurately prepared by following ISO and serial dilution schemes. However, itraconazole trays showed a significant lack of reproducibility that was independent of which method was followed.

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Source
http://dx.doi.org/10.1128/JCM.00123-10DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2863860PMC
May 2010

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