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Heliyon 2021 Jan 27;7(1):e06053. Epub 2021 Jan 27.

ITER-India, Institute for Plasma Research, Ahmedabad, 380005, India.

The constantly increasing energy consumption along with the depleting fossil fuel resources as well as owing to the fact that the nuclear fission not being an intrinsically safe method of energy generation, it has become necessary to look for other solutions to fulfil the future energy demands. Nuclear fusion, the source of energy for billions of stars, has attracted the attention of scientists and engineers despite a lot of technical challenges in the replication of the fusion process in laboratories. For fusion to take place in a device, one of the major challenges faced is the strong magnetic confinement of the plasma using large superconducting (SC) magnets, which need efficient cryogenic cooling techniques to maintain the required low temperatures for the superconducting state. Read More

J Microbiol Methods 2016 11 25;130:83-91. Epub 2016 Aug 25.

Ajinomoto-Genetika Research Institute, 117545 Moscow, Russian Federation.

Despite the abundance of genetic manipulation approaches, particularly for Escherichia coli, new techniques and increased flexibility in the application of existing techniques are required to address novel aims. The most widely used approaches for chromosome editing are based on bacteriophage site-specific and λRed/RecET-mediated homologous recombination. In the present study, these techniques were combined to develop a novel approach for in vivo cloning and targeted long-length chromosomal insertion. Read More

J Chem Phys 2016 Apr;144(15):154901

U.S. Army Research Laboratory, Aberdeen Proving Ground, Maryland 21005, USA.

Equilibrated systems of entangled polymer melts cannot be produced using direct brute force equilibration due to the slow reptationdynamics exhibited by high molecular weight chains. Instead, these dense systems are produced using computational techniques such as Monte Carlo-Molecular Dynamics hybrid algorithms, though the use of soft potentials has also shown promise mainly for coarse-grained polymeric systems. Through the use of soft-potentials, the melt can be equilibrated via molecular dynamics at intermediate and long length scales prior to switching to a Lennard-Jones potential. Read More

IEEE Trans Appl Supercond 2010 Jun 19;20(3):756-759. Epub 2010 Apr 19.

FBML/MIT, Cambridge, MA 02139 USA.

In this paper, we report our progress in the development of a low cost, liquid-helium-free MgB superconducting MRI magnet. Several technical issues related to the construction of such a magnet to operate in persistent current mode are discussed, namely, the performance consistency of long length MgB conductors, the fabrication of superconducting joints and persistent current switches. Quench detection and protection of such magnets operated in solid nitrogen environment are also discussed. Read More

Nucleic Acids Res 2010 May 1;38(8):2594-602. Epub 2010 Mar 1.

Codon Devices, Inc., One Kendall Square, Building 300, Cambridge, MA 02139, USA.

The engineering of biological components has been facilitated by de novo synthesis of gene-length DNA. Biological engineering at the level of pathways and genomes, however, requires a scalable and cost-effective assembly of DNA molecules that are longer than approximately 10 kb, and this remains a challenge. Here we present the development of pairwise selection assembly (PSA), a process that involves hierarchical construction of long-length DNA through the use of a standard set of components and operations. Read More