Centrifugo-pneumatic valve for metering of highly wetting liquids on centrifugal microfluidic platforms.

Authors:
Daniel Mark
Daniel Mark
Duke Clinical Research Institute
United States
Tobias Metz
Tobias Metz
University of Freiburg
Germany
Stefan Haeberle
Stefan Haeberle
HSG-IMIT-Institute for Micromachining and Information Technology
Sascha Lutz
Sascha Lutz
Roche Diagnostics GmbH
Mannheim | Germany
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Fraunhofer Project Centre at Dublin City University
Professor (Full)
microfluidics, Lab-on-a-Chip, hydrodynanmics, business development, project management, organisational leadership
Glasnevin, Dublin 9 | Ireland
Roland Zengerle
Roland Zengerle
University of Freiburg
Germany

Lab Chip 2009 Dec 12;9(24):3599-603. Epub 2009 Oct 12.

HSG-IMIT - Institut für Mikro- und Informationstechnik, Wilhelm-Schickard-Strasse 10, D-78052 Villingen-Schwenningen, Germany.

We designed and experimentally validated a new type of passive valve for centrifugal microfluidic platforms. A liquid column entering an unvented receiving chamber is stopped by the counter-pressure of compressed air. This valve opens under defined conditions at high centrifugal frequencies at which the interface between liquid and air becomes unstable and enables a phase exchange, forwarding the liquid. Burst frequencies of the valve were determined for liquids typically used in biochemical assays: pure water, water with detergent concentrations between 0.01 and 10%, and pure ethanol. Burst frequencies between 8.5 +/- 0.6 and 27.9 +/- 2.0 Hz were measured for different surface tensions. The burst frequencies can be tuned by simple geometrical changes in the valving structure. The valve does not require ultra-precise structures or local surface modifications and is therefore ideal for low-cost microfluidic polymer disks. Potential applications are in the field of multiparameter and panel analysis, such as PCR-genotyping.

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December 2009
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