Gene expression before HAART initiation predicts HIV-infected individuals at risk of poor CD4+ T-cell recovery.

AIDS 2010 Jan;24(2):217-22

Department of Medicine, University of California, San Diego, La Jolla, USA.

Objective: To identify a pre-HAART gene expression signature in peripheral blood mononuclear cells (PBMCs) predictive of CD4 T-cell recovery during HAART in HIV-infected individuals.

Design: This retrospective study evaluated PBMC gene expression in 24 recently HIV-infected individuals before the initiation of HAART to identify genes whose expression is predictive of CD4 T-cell recovery after 48 weeks of HAART.

Methods: The change in CD4 T-cell count (DeltaCD4) over the 48-week study period was calculated for each of the 24 participants. Twelve participants were assigned to the 'good' (DeltaCD4 > or = 200 cells/microl) and 12 to the 'poor' (DeltaCD4 < 200 cells/microl) CD4 T-cell recovery group. Gene expression profiling of the entire transcriptome using Illumina BeadChips was performed with PBMC samples obtained before HAART. Gene expression classifiers capable of predicting CD4 T-cell recovery group (good vs. poor), as well as the specific DeltaCD4 value, at week 48 were constructed using methods of Class Prediction.

Results: The expression of 40 genes in PBMC samples taken before HAART predicted CD4 T-cell recovery group (good vs. poor) at week 48 with 100% accuracy. The expression of 22 genes predicted a specific DeltaCD4 value for each HIV-infected individual that correlated well with actual values (R = 0.82). Predicted DeltaCD4 values were also used to assign individuals to good vs. poor CD4 T-cell recovery groups with 79% accuracy.

Conclusion: Gene expression in PBMCs can be used as biomarkers to successfully predict disease outcomes among HIV-infected individuals treated with HAART.

Download full-text PDF

Source
https://insights.ovid.com/crossref?an=00002030-201001030-000
Publisher Site
http://dx.doi.org/10.1097/QAD.0b013e328334f1f0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2915828PMC
January 2010

Publication Analysis

Top Keywords

cd4 t-cell
28
t-cell recovery
28
gene expression
24
recovery group
12
hiv-infected individuals
12
good poor
12
specific deltacd4
8
group good
8
expression
8
samples haart
8
pbmc samples
8
expression genes
8
predictive cd4
8
t-cell
8
deltacd4 200
8
200 cells/microl
8
recovery
7
cd4
7
gene
6
haart
6

Similar Publications