Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA.

Nat Methods 2010 Jan 29;7(1):47-9. Epub 2009 Nov 29.

Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations.

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http://dx.doi.org/10.1038/nmeth.1404DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862482PMC
January 2010
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