Blueberry fruit polyphenolics suppress oxidative stress-induced skeletal muscle cell damage in vitro.

Mol Nutr Food Res 2010 Mar;54(3):353-63

Functional Food and Health Group, New Zealand Institute for Plant and Food Research Ltd., Hamilton, New Zealand.

Skeletal muscle damage can result from disease and unaccustomed or excessive exercise. Muscle dysfunction occurs via an increased level of reactive oxygen species and hence there is potential in antioxidants as amelioration strategies. We explored the putative benefit of fruit polyphenolic extracts in reducing the susceptibility of skeletal muscle cells to oxidative stress. Muscle myotubes were simultaneously challenged with fruit extracts (1-50 microg/mL) and calcium ionophore (A23187), hydrogen peroxide, or 2,4-dinitrophenol and damage monitored by release of cytosolic enzymes. A blueberry fruit extract displayed a potent and significant dose-dependent protective capacity. Evaluation of the protective capacity of anthocyanin sub-extracts of blueberry fruit and pure individual glycosides, with identification of extract polyphenolic components using MS, suggested that malvidin galactoside and/or glucoside were the active compounds. These in vitro data support the concept that blueberry fruits or derived foods rich in malvidin glycosides may be beneficial in alleviating muscle damage caused by oxidative stress. More research on the benefits of blueberry fruit consumption in human intervention studies is warranted.

Download full-text PDF

Source
http://dx.doi.org/10.1002/mnfr.200900094DOI Listing
March 2010
2 Reads

Publication Analysis

Top Keywords

blueberry fruit
16
skeletal muscle
12
protective capacity
8
oxidative stress
8
muscle damage
8
muscle
6
fruit
5
blueberry
5
microg/ml calcium
4
blueberry fruits
4
extracts 1-50
4
concept blueberry
4
1-50 microg/ml
4
calcium ionophore
4
hydrogen peroxide
4
peroxide 24-dinitrophenol
4
a23187 hydrogen
4
data support
4
ionophore a23187
4
support concept
4

References

(Supplied by CrossRef)
Exercise-induced oxidative stress
Alessio et al.
Med. Sci. Sports Exerc. 1993

McArdle et al.
1997
The mitochondrial site of superoxide formation
Nohl et al.
Biochem. Biophys. Res. Commun. 1986
Free radicals in cell biology
Djordjevic et al.
Int. Rev. Cytol. Surv. Cell Biol. 2004
Cyanidins: metabolism and biological properties
Galvano et al.
J. Nutri. Biochem. 2004
Anthocyanins and anthocyanin-rich extracts in biology and medicine. Biochemical, cellular and medicinal properties
Ghosh et al.
Curr. Top. Nutraceut. Res. 2005

Similar Publications