Indian J Exp Biol 2007 Jan;45(1):48-57
Department of Biology, W M Keck Center for Cellular Imaging, Gilmer Hall, University of Virginia, Charlottesville, VA 22904, USA.
Microscopy has become an essential tool for cellular protein investigations. The development of new fluorescent markers such as green fluorescent proteins generated substantial opportunities to monitor protein-protein interactions qualitatively and quantitatively using advanced fluorescence microscope techniques including wide-field, confocal, multiphoton, spectral imaging, lifetime, and correlation spectroscopy. The specific aims of the investigation of protein dynamics in live specimens dictate the selection of the microscope methodology. Read More