Complement modulates pathogenesis and antibody-dependent neutralization of West Nile virus infection through a C5-independent mechanism.

Virology 2009 Oct 9;393(1):11-5. Epub 2009 Sep 9.

Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Although the interactions of complement and viruses have been widely studied, the function of C5 and the membrane attack complex in the context of viral infection or antibody-mediated neutralization remains controversial. Using C5-depleted or -deficient human or mouse sera, we show that C5 does not contribute to the antibody-dependent or -independent neutralization of West Nile virus (WNV) in cell culture. Consistent with this, C5 neither contributed to protection against WNV pathogenesis nor augmented the neutralizing efficacy of complement-fixing anti-WNV neutralizing antibodies in mice. Although previous studies established that activation of the classical, lectin, and alternative complement pathways restricts WNV infection, our results show little effect of C5 and by inference the terminal lytic complement components. Overall, these results enhance our mechanistic understanding of how complement controls flavivirus infections.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.virol.2009.08.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2753729PMC
October 2009
5 Reads

Publication Analysis

Top Keywords

nile virus
8
neutralization west
8
west nile
8
complement
5
wnv cell
4
virus wnv
4
-independent neutralization
4
antibody-dependent -independent
4
cell culture
4
restricts wnv
4
culture consistent
4
wnv infection
4
infection inference
4
controversial c5-depleted
4
inference terminal
4
human mouse
4
-deficient human
4
terminal lytic
4
mouse sera
4
established activation
4

Similar Publications