A novel murine model for localized radiation necrosis and its characterization using advanced magnetic resonance imaging.

Int J Radiat Oncol Biol Phys 2009 Oct;75(2):527-33

Department of Neurosurgery, Washington University School of Medicine, St. Louis, MO 63110, USA.

Purpose: To develop a murine model of radiation necrosis using fractionated, subtotal cranial irradiation; and to investigate the imaging signature of radiation-induced tissue damage using advanced magnetic resonance imaging techniques.

Methods And Materials: Twenty-four mice each received 60 Gy of hemispheric (left) irradiation in 10 equal fractions. Magnetic resonance images at 4.7 T were subsequently collected using T1-, T2-, and diffusion sequences at selected time points after irradiation. After imaging, animals were killed and their brains fixed for correlative histologic analysis.

Results: Contrast-enhanced T1- and T2-weighted magnetic resonance images at months 2, 3, and 4 showed changes consistent with progressive radiation necrosis. Quantitatively, mean diffusivity was significantly higher (mean = 0.86, 1.13, and 1.24 microm(2)/ms at 2, 3, and 4 months, respectively) in radiated brain, compared with contralateral untreated brain tissue (mean = 0.78, 0.82, and 0.83 microm(2)/ms) (p < 0.0001). Histology reflected changes typically seen in radiation necrosis.

Conclusions: This murine model of radiation necrosis will facilitate investigation of imaging biomarkers that distinguish between radiation necrosis and tumor recurrence. In addition, this preclinical study supports clinical data suggesting that diffusion-weighted imaging may be helpful in answering this diagnostic question in clinical settings.

Download full-text PDF

Source
http://linkinghub.elsevier.com/retrieve/pii/S036030160900854
Publisher Site
http://dx.doi.org/10.1016/j.ijrobp.2009.06.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3808839PMC
October 2009
3 Reads

Publication Analysis

Top Keywords

radiation necrosis
20
magnetic resonance
16
murine model
12
model radiation
8
resonance images
8
advanced magnetic
8
resonance imaging
8
imaging
6
radiation
6
necrosis
5
higher 086
4
diffusivity higher
4
progressive radiation
4
necrosis quantitatively
4
quantitatively diffusivity
4
086 113
4
124 microm2/ms
4
compared contralateral
4
contralateral untreated
4
untreated brain
4

Similar Publications