In vivo properties of the proangiogenic peptide QK.

Authors:
Gaetano Santulli
Gaetano Santulli
Federico II University of Naples
Italy
Michele Ciccarelli
Michele Ciccarelli
University of Salerno
Fisciano | Italy
Alfonso Campanile
Alfonso Campanile
Ospedale Maggiore Policlinico
Gennaro Galasso
Gennaro Galasso
Federico II University
Italy
Barbara Ziaco
Barbara Ziaco
Università di Napoli Federico II
Italy
Giovanna Giuseppina Altobelli
Giovanna Giuseppina Altobelli
School of Medicine and Surgery
Indianapolis | United States
Vincenzo Cimini
Vincenzo Cimini
Medical School
Boston | United States

J Transl Med 2009 Jun 8;7:41. Epub 2009 Jun 8.

Dipartimento di Medicina Clinica, Scienze Cardiovascolari ed Immunologiche, Cattedra di Medicina Interna, Università degli Studi Federico II di Napoli, Italy.

The main regulator of neovascularization is Vascular Endothelial Growth Factor (VEGF). We recently demonstrated that QK, a de novo engineered VEGF mimicking peptide, shares in vitro the same biological properties of VEGF, inducing capillary formation and organization. On these grounds, the aim of this study is to evaluate in vivo the effects of this small peptide. Therefore, on Wistar Kyoto rats, we evaluated vasomotor responses to VEGF and QK in common carotid rings. Also, we assessed the effects of QK in three different models of angiogenesis: ischemic hindlimb, wound healing and Matrigel plugs. QK and VEGF present similar endothelium-dependent vasodilatation. Moreover, the ability of QK to induce neovascularization was confirmed us by digital angiographies, dyed beads dilution and histological analysis in the ischemic hindlimb as well as by histology in wounds and Matrigel plugs. Our findings show the proangiogenic properties of QK, suggesting that also in vivo this peptide resembles the full VEGF protein. These data open to new fields of investigation on the mechanisms of activation of VEGF receptors, offering clinical implications for treatment of pathophysiological conditions such as chronic ischemia.

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Source
http://dx.doi.org/10.1186/1479-5876-7-41DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2702279PMC

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June 2009
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