Mocr: a novel fusion tag for enhancing solubility that is compatible with structural biology applications.

Protein Expr Purif 2009 Jan 12;63(1):40-9. Epub 2008 Sep 12.

High-throughput Protein Lab, Center for Structural Biology, Life Sciences Institute, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, Michigan 48109, USA.

A persistent problem in heterologous protein production is insolubility of the target protein when expressed to high level in the host cell. A widely employed strategy for overcoming this problem is the use of fusion tags. The best fusion tags promote solubility, may function as purification handles and either do not interfere with downstream applications or may be removed from the passenger protein preparation. A novel fusion tag is identified that meets these criteria. This fusion tag is a monomeric mutant of the Ocr protein (0.3 gene product) of bacteriophage T7. This fusion tag displays solubilizing activity with a variety of different passenger proteins. We show that it may be used as a purification handle similar to other fusion tags. Its small size and compact structure are compatible with its use in downstream applications of the passenger protein or it may be removed and purified away from the passenger protein. The use of monomeric Ocr (Mocr) as a complement to other fusion tags such as maltose-binding protein will provide greater flexibility in protein production and processing for a wide variety of protein applications.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.pep.2008.08.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2936452PMC
January 2009
8 Reads

Publication Analysis

Top Keywords

fusion tag
16
fusion tags
16
passenger protein
12
protein
9
downstream applications
8
protein production
8
fusion
8
novel fusion
8
tag identified
4
preparation novel
4
mocr complement
4
protein preparation
4
ocr mocr
4
identified meets
4
protein monomeric
4
tag monomeric
4
criteria fusion
4
meets criteria
4
monomeric ocr
4
removed passenger
4

Altmetric Statistics

Similar Publications