Anal Biochem 2008 Sep 22;380(1):146-8. Epub 2008 May 22.
Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, A-1090 Vienna, Austria.
Baculoviruses are widely used for protein production in insect cells, and their potential for gene transfer to mammalian cells is increasingly being recognized. Here we describe a baculovirus vector with a bicistronic mammalian expression cassette and demonstrate its suitability for efficient transient and stable protein expression in human glioblastoma cells. Bicistronic baculovirus vectors are safe, cost efficient, and easy to produce; thus, they represent an excellent gene transfer system for mammalian cells.