J Microencapsul 2008 Jun;25(4):267-74
HSG-IMIT-Institute for Micromachining and Information Technology, Villingen-Schwenningen, Germany.
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J Microencapsul 2002 Jul-Aug;19(4):415-24
FMC Biopolymer, Gaustadalléen 21, N-0349 Oslo, Norway.
The effect of several parameters on the size of alginate beads produced by use of an electrostatic potential bead generator was examined. Parameters studied included needle diameter, electrostatic potential, alginate solution flow rate, gelling ion concentration and alginate concentration and viscosity, as well as alginate composition. Bead size was found to decrease with increasing electrostatic potential, but only down to a certain level. Read More
J Microencapsul 2002 May-Jun;19(3):273-80
Martin-Luther-Universität Halle-Wittenberg, Fachbereich Ingenieurwissenschaften, Institut für Bioengineering, Germany.
The JetCutter technology originally developed for high-throughput encapsulation of particles and substances into small beads was applied in this study for the entrapment of mammalian cells in alginate beads. In contrast to other established techniques such as the air jet droplet generation or laminar jet break-up, the JetCutter is capable of working with highly viscous fluids necessary for the production of stable beads based on hydrogels. A 1. Read More
J Biotechnol 2004 Nov;114(3):315-26
Institute of Biotechnology, Swiss Federal Institute of Technology, ETH Hoenggerberg, CH-8093 Zurich, Switzerland.
Microencapsulation of desired mammalian cell phenotypes in biocompatible polymer matrices represents a powerful technology for cell-based therapies and biopharmaceutical manufacturing of protein therapeutics. We have pioneered a novel jet break-up-compatible process for encapsulation of mammalian cells in cellulose sulfate (CS)/poly-diallyl-dimethyl-ammoniumchloride (pDADMAC) (CellMAC) capsules. CS and pDADMAC polymerize on a transient ad hoc co-assembled Ca2+/alginate scaffold and form homogenous capsules following dissolution of the alginate core by Ca2+ chelating agents. Read More
J Colloid Interface Sci 2007 Aug 19;312(2):247-55. Epub 2007 Apr 19.
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117576 Singapore.
Entrapment of living cells in microbeads is to protect the encapsulated cells from the host's immune system, which can be used as drug delivery vehicles, immunotherapies and engineered tissues. The main objective of the present study was to investigate the droplet formation and to better develop mono-dispersed microencapsulation of living cells with controllable size. The uniformity of microencapsulation size was realized by performing electrospray in the dripping mode and also stabilized by an additional ring electrode. Read More