Quantification of atrazine and its metabolites in urine by on-line solid-phase extraction-high-performance liquid chromatography-tandem mass spectrometry.

Anal Bioanal Chem 2008 Jul 3;391(5):1931-9. Epub 2008 May 3.

Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Hwy, N.E., MS F-17, Atlanta, GA, 30360, USA.

We have developed a method using on-line solid-phase extraction-high-performance liquid chromatography-tandem mass spectrometry (SPE-HPLC-MS/MS) and isotope dilution quantification to measure atrazine and seven atrazine metabolites in urine. The metabolites measured were hydroxyatrazine, diaminochloroatrazine, desisopropylatrazine, desethylatrazine, desethylatrazine mercapturate, atrazine mercaturate and atrazine itself. Our method has good precision (relative standard deviations ranging from 4 to 20% at 5, 10 and 50 ng/mL), extraction efficiencies of 67 to 102% at 5 and 25 ng/mL, relative recoveries of 87 to 112% at 5, 25, 50 and 100 ng/mL limits of detection (LOD) ranging from 0.03 to 2.80 ng/mL. The linear range of our method spans from the analyte LOD to 100 ng/mL (40 ng/mL for atrazine and atrazine mercapturate) with R (2) values of greater than 0.999 and errors about the slope of less than 3%. Our method is rapid, cost-effective and suitable for large-scale sample analyses and is easily adaptable to other biological matrices. More importantly, this method will allow us to better assess human exposure to atrazine-related chemicals.

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http://dx.doi.org/10.1007/s00216-008-2102-0DOI Listing
July 2008
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References

(Supplied by CrossRef)

CDS Tomlin et al.
1997

RT Mandelbaum et al.
Appl Environ Microbiol 1993

AJ Roos De et al.
Occup Environ Med 2003

PA Hessel et al.
J Occup Environ Med 2004

ZS Hoar et al.
Scand J Work Environ Health 1993

C Hopenhayn-Rich et al.
Arch Environ Contam Toxicol 2002

JA Hoppin et al.
Am J Respir Crit Care Med 2002

PA MacLennan et al.
J Toxicol Environ Health A 2003

PA MacLennan et al.
J Occup Environ Med 2002

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