Simultaneous analysis of riboflavin and aromatic amino acids in beer using fluorescence and multivariate calibration methods.

Authors:
Ewa Sikorska
Ewa Sikorska
Poznań University of Economics
Poland
Dr. Igor Khmelinskii, PhD, Prof. Agregado
Dr. Igor Khmelinskii, PhD, Prof. Agregado
University of the Algarve
PhD, Prof. Agregado
physcal chemistry; spectroscopy; climate science
Faro, Algarve | Portugal
Marek Sikorski
Marek Sikorski
A. Mickiewicz University
Poland

Anal Chim Acta 2008 Apr 6;613(2):207-17. Epub 2008 Mar 6.

Faculty of Commodity Science, The Poznań University of Economics, al. Niepodległości 10, 60-967 Poznań, Poland.

The study demonstrates an application of the front-face fluorescence spectroscopy combined with multivariate regression methods to the analysis of fluorescent beer components. Partial least-squares regressions (PLS1, PLS2, and N-way PLS) were utilized to develop calibration models between synchronous fluorescence spectra and excitation-emission matrices of beers, on one hand, and analytical concentrations of riboflavin and aromatic amino acids, on the other hand. The best results were obtained in the analysis of excitation-emission matrices using the N-way PLS2 method. The respective correlation coefficients, and the values of the root mean-square error of cross-validation (RMSECV), expressed as percentages of the respective mean analytic concentrations, were: 0.963 and 14% for riboflavin, 0.974 and 4% for tryptophan, 0.980 and 4% for tyrosine, and 0.982 and 19% for phenylalanine.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aca.2008.02.063DOI Listing

Still can't find the full text of the article?

We can help you send a request to the authors directly.
April 2008
23 Reads
1 Citation
4.510 Impact Factor

Publication Analysis

Top Keywords

aromatic amino
8
amino acids
8
riboflavin aromatic
8
excitation-emission matrices
8
matrices n-way
4
pls1 pls2
4
error cross-validation
4
pls2 n-way
4
analysis excitation-emission
4
pls utilized
4
14% riboflavin
4
n-way pls
4
regressions pls1
4
riboflavin 0974
4
percentages respective
4
partial least-squares
4
components partial
4
n-way pls2
4
beer components
4
least-squares regressions
4

Similar Publications