A novel orf108 co-transcribed with the atpA gene is associated with cytoplasmic male sterility in Brassica juncea carrying Moricandia arvensis cytoplasm.

Plant Cell Physiol 2008 Feb 4;49(2):284-9. Epub 2008 Jan 4.

National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi-110012, India.

Mitochondrial atpA transcripts were examined in cytoplasmic male sterile (CMS) and fertility restorer lines of CMS (Moricandia arvensis) Brassica juncea. Male sterile flowers had longer atpA transcripts than male fertiles. The mitochondrial atpA region of the CMS line was cloned and sequenced. The 5' and 3' ends of the atpA transcripts of the CMS and the fertility restorer lines were mapped and full-length transcripts were cloned and sequenced. A novel orf108 (open reading frame 108) co-transcribed with the atpA gene was found in the male sterile flowers. In the fertility restorer line, the transcript was cleaved within orf108 to yield monocistronic atpA transcripts.

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http://dx.doi.org/10.1093/pcp/pcm182DOI Listing
February 2008
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References

(Supplied by CrossRef)

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 2006

Brown et al.
Journal of Heredity 1999

Dombrowski et al.
Journal of Biological Chemistry 1999

PLANT BREED 2006

The Plant Cell Online 2004

PROG BOT 2006

Genetics 1996

Kuhn et al.
Nucleic Acids Research 2002

Liu et al.
Annual review of genetics 2006

L'Homme et al.
Current genetics 1997

Pathania et al.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 2003

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