Clin Endocrinol (Oxf) 2007 Sep 28;67(3):342-5. Epub 2007 May 28.
Arthritis Research Campaign Epidemiology Unit (ARC/EU) and Centre for Molecular Medicine (CMM), University of Manchester, Manchester, UK.
Background: A defect in hypothalamic-pituitary-adrenal (HPA) axis function has been suggested to contribute to susceptibility to rheumatoid arthritis (RA).
Objective: To investigate polymorphisms of the glucocorticoid receptor (GR) gene and determine any associations with RA.
Methods: Three GR polymorphisms that tag 95% of all haplotypes across the GR gene were genotyped. These are an intron B Bcl1 polymorphism, a ttg insertion/deletion within intron F (rs2307674) and the single nucleotide polymorphism (SNP) lying in the 3' untranslated region of exon 9b (rs6198). The dye terminator-based SNaPshot method or size resolution by capillary electrophoresis was performed. The study population comprised 198 UK Caucasian RA cases and 393 ethnically matched controls.
Results: No significant single point or haplotypic associations were found for GR polymorphisms with RA susceptibility. Furthermore, no evidence for GR polymorphisms with aspects of RA severity was seen.
Conclusion: In this study of the most comprehensive coverage of GR polymorphisms with RA, no significant contributing role for GR polymorphisms with RA was found.