Ribosomal tethering and clustering as mechanisms for translation initiation.

Proc Natl Acad Sci U S A 2006 Nov 16;103(48):18077-82. Epub 2006 Nov 16.

Department of Neurobiology, The Scripps Research Institute, La Jolla, CA 92037, USA.

Eukaryotic mRNAs often recruit ribosomal subunits some distance upstream of the initiation codon; however, the mechanisms by which they reach the initiation codon remain to be fully elucidated. Although scanning is a widely accepted model, evidence for alternative mechanisms has accumulated. We previously suggested that this process may involve tethering of ribosomal complexes to the mRNA, in which the intervening mRNA is bypassed, or clustering, in which the initiation codon is reached by dynamic binding and release of ribosomal subunits at internal sites. The present studies tested the feasibility of these ideas by using model mRNAs and revealed that translation efficiency varied with the distance between the site of ribosomal recruitment and the initiation codon. The present studies also showed that translation could initiate efficiently at AUG codons located upstream of an internal site. These observations are consistent with ribosomal tethering at the cap structure and clustering at internal sites.

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.0608212103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1838709PMC
November 2006
2 Reads

Publication Analysis

Top Keywords

initiation codon
16
ribosomal tethering
8
ribosomal subunits
8
internal sites
8
ribosomal
6
initiation
5
mrna bypassed
4
clustering initiation
4
bypassed clustering
4
codon reached
4
efficiently aug
4
reached dynamic
4
intervening mrna
4
aug codons
4
complexes mrna
4
located upstream
4
suggested process
4
ribosomal recruitment
4
accumulated suggested
4
process involve
4

Altmetric Statistics

References

(Supplied by CrossRef)

Pisarev et al.
Comptes rendus biologies 2005

Kozak et al.
Gene 2002

Sedman et al.
Journal of Virology 1990

Yueh et al.
Genes & Development 2000

Ryabova et al.
Progress in nucleic acid research and molecular biology 2002

PNAS 2004

Lammich et al.
EMBO Reports 2004

Slusher et al.
PNAS 1991

RNA 2006

Preiss et al.
Nature; Physical Science (London) 1998

Kaminski et al.
The EMBO Journal 1994

Similar Publications