Centrifugal extraction of plasma from whole blood on a rotating disk.

Authors:
Stefan Haeberle
Stefan Haeberle
HSG-IMIT-Institute for Micromachining and Information Technology
Thilo Brenner
Thilo Brenner
IMTEK-University of Freiburg
Freiburg | Germany
Roland Zengerle
Roland Zengerle
University of Freiburg
Germany
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Fraunhofer Project Centre at Dublin City University
Professor (Full)
microfluidics, Lab-on-a-Chip, hydrodynanmics, business development, project management, organisational leadership
Glasnevin, Dublin 9 | Ireland

Lab Chip 2006 Jun 13;6(6):776-81. Epub 2006 Apr 13.

Laboratory for MEMS Applications, Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Koehler-Allee 106, D-79110 Freiburg, Germany.

We present a centrifugal process for the extraction of plasma from sediment by a decanting structure, terminating with metered plasma which is readily available for subsequent on-disk processing. Our technique supplies 2 microl plasma from 5 microl of whole blood at moderate spinning frequencies of 40 Hz within 20 s, only. The residual cell concentration in the purified plasma amounts to less than 0.11%, independent of the frequency of rotation. A capillary duct connects the extracted plasma to subsequent on-disk processing units.

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June 2006
4 Reads
26 PubMed Central Citations(source)

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