Effect of portal vein occlusion on the pancreas: an experimental model.

World J Surg 2006 Jun;30(6):1000-6

Department of Surgery, Celal Bayar University Medical Faculty, Manisa, Turkey.

Background: The effects of portal vein occlusion on the pancreas are not clearly understood. Therefore, we studied histomorphological changes induced in the rat pancreas by various periods of portal vein occlusion.

Materials And Methods: Sixty female Wistar albino rats were randomly allocated into four groups of 15 each. In Group I (control), rats underwent sham laparotomy to expose the portal vein proximal to its bifurcation. In Groups II-IV, rats underwent laparotomy followed by portal vein occlusion by clamping for 15, 30, and 60 minutes respectively. The pancreas was removed immediately after sham laparotomy in Group I and immediately after clamp release in Groups II-IV. Pancreatic tissue specimens were subjected to histochemical analysis for cell typing and diagnosis, immunohistochemical analysis for identification of the inflammatory markers tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), endothelial nitric oxide synthase (eNOS), and inducible NOS (iNOS), and TUNEL analysis was carried out for identification of apoptotic cells.

Results: Histochemistry revealed signs of inflammation in pancreatic tissue from rats subjected to portal vein occlusion. Immunohistochemistry revealed that the expression of proinflammatory cytokines TNF-alpha and IL-1beta and the oxidative damage indicator iNOS in rat pancreatic tissue increased progressively with the duration of portal vein occlusion. TUNEL assay revealed no signs of apoptosis in any of the groups.

Conclusion: We conclude that portal vein occlusion triggers an inflammatory response in the pancreas that worsens the longer the occlusion lasts.

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http://dx.doi.org/10.1007/s00268-005-7883-0DOI Listing
June 2006
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References

(Supplied by CrossRef)

CA Dinarello et al.
Blood 1996

RM Strieter et al.
Crit Care Med 1993

S Karne et al.
Surg Clin North Am 1999

F Serracino-Inglott et al.
Am J Surg 2001

JM Badia et al.
Eur J Surg 1998

HC Lin et al.
Hepatology 1996

R Tomaszewska et al.
Pol J Pathol 2000

CA Camargo Jr et al.
J Am Coll Surg 1995

NV Krokos et al.
Transpl Int 1995

B Eghtesad et al.
Transplantation 2003

S Sathasivam et al.
ANZ J Surg 2004

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