Detection of serum antibodies to hepatitis C virus in 'false-seronegative' blood donors in Oman.

Med Princ Pract 2006 ;15(2):111-3

Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, Al-Khod, Oman.

Objective: To detect hepatitis C virus (HCV) antibodies in seronegative donors by disruption of the immune complexes (ICs).

Subjects And Methods: HCV antibody detection was carried out on 600 seronegative donors following an IC dissociation assay. Reverse transcription polymerase chain reaction (RT-PCR) was then performed on the positive results.

Results: Nine of the 600 samples (1.5%) were positive for IC-dissociated HCV antibodies. Of the 9 only 3 antibody-positive samples had detectable HCV RNA.

Conclusion: Screening for antibodies to HCV in combination with PCR appears to be the safest way to reduce the residual risk of HCV in blood transfusion.

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Source
http://dx.doi.org/10.1159/000090914DOI Listing
July 2006

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