Immune complex deposition in adult male Sprague-Dawley rats chronically immunized with GnRH.

Am J Reprod Immunol 2005 Nov;54(5):292-310

Population Council, Center for Biomedical Research, The Rockefeller University, New York, NY 10021, USA.

Problem: This study was undertaken to evaluate whether the anti-GnRH antibodies and immune complexes (IC) generated by immunization with GnRH-TT cause cellular damage within the animal.

Method Of Study: Chronic immunization of rats with GnRH-TT injected i.m. was followed by tissue/organ analysis for immune complex deposition by immunofluorescence microscopy. Two groups were studied: (1) those immunized throughout the experiment until their ultimate demise, and (2) those given a chance to recover from the effects of chronic immunization before final analysis.

Results: GnRH-TT was effective in stopping spermatogenesis, which resumed after withdrawal of the immunogen. Most tissues from chronically immunized animals were not significantly different than controls, however the kidneys of treated animals exhibited a higher accumulation of IC. Despite increased IC deposition, pathologic effects were not detected at the cellular level.

Conclusions: GnRH-TT is an effective immunocontraceptive although the accumulation of glomerular IC represents a potential deleterious side effect.

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http://dx.doi.org/10.1111/j.1600-0897.2005.00310.xDOI Listing
November 2005
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