Substance P activates p38 mitogen-activated protein kinase to promote IL-6 induction in human dental pulp fibroblasts.

Connect Tissue Res 2005 ;46(3):153-8

Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.

Substance P (SP) induces the expression of proinflammatory cytokines, such as interleukin (IL)-6, which are implicated in pulp inflammation. To determine the signal pathway of SP-induced IL-6, we examined the activities of the mitogen-activated protein kinases (MAPKs) in human dental pulp cell (PF-10) cultures. SP induced the phosphorylation of p38 MAPK within 5 min; this activation persisted for up to 40 min and was independent of the activation of extracellular signal-related kinases (ERK-1 and ERK-2) that were induced after SP stimulation of PF-10 cells. As shown by electrophoretic mobility shift assay p38 MAPK was not involved in SP-induced activation of nuclear factor-kappa B (NF-kappaB). However, p38 MAPK mediated SP-induced IL-6 production, as shown by the use of specific inhibitors of this kinase. Our results suggest that the activation of p38 MAPK is important for NF-kappaB-independent regulator of neurogenic inflammation in dental pulp tissues.

Download full-text PDF

Source
http://dx.doi.org/10.1080/03008200500182490DOI Listing
October 2005
5 Reads

Publication Analysis

Top Keywords

p38 mapk
16
dental pulp
12
human dental
8
mitogen-activated protein
8
sp-induced il-6
8
p38
5
activation extracellular
4
independent activation
4
signal-related kinases
4
extracellular signal-related
4
kinases erk-1
4
stimulation pf-10
4
pf-10 cells
4
induced stimulation
4
erk-2 induced
4
erk-1 erk-2
4
min independent
4
min activation
4
pf-10 cultures
4
cultures induced
4

References

(Supplied by CrossRef)

Similar Publications