Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map.

Genetics 2005 Nov 22;171(3):1305-9. Epub 2005 Aug 22.

Departament de Genètica Vegetal, Laboratori de Genètica Molecular Vegetal, CSIC-IRTA, 08348 Cabrils (Barcelona), Spain.

The concept of selective (or bin) mapping is used here for the first time, using as an example the Prunus reference map constructed with an almond x peach F2 population. On the basis of this map, a set of six plants that jointly defined 65 possible different genotypes for the codominant markers mapped on it was selected. Sixty-three of these joint genotypes corresponded to a single chromosomal region (a bin) of the Prunus genome, and the two remaining corresponded to two bins each. The 67 bins defined by these six plants had a 7.8-cM average length and a maximum individual length of 24.7 cM. Using a unit of analysis composed of these six plants, their F1 hybrid parent, and one of the parents of the hybrid, we mapped 264 microsatellite (or simple-sequence repeat, SSR) markers from 401 different microsatellite primer pairs. Bin mapping proved to be a fast and economic strategy that could be used for further map saturation, the addition of valuable markers (such as those based on microsatellites or ESTs), and giving a wider scope to, and a more efficient use of, reference mapping populations.

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http://www.genetics.org/cgi/doi/10.1534/genetics.105.043661
Publisher Site
http://dx.doi.org/10.1534/genetics.105.043661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1456826PMC
November 2005
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References

(Supplied by CrossRef)

Theor. Appl. Genet. 2003

J. Hered. 1990

Mol. Ecol. Notes 2004

J. Am. Soc. Hortic. Sci. 2003

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