[Construction, Expression, Purification and Antiviral Activity of Fused Interferon-BLA (IFN-BLA).].

Yi Chuan 2005 May;27(3):451-6

Shanghai CASB Biotechnology Co., Chinese Academy of Scieneces, Shanghai 200233, China, Email:

A fusion protein, Interferon-BLA (IFN-BLA), was constructed with IFN-beta-1b and IFN-alpha-2b separating by a linker -GGGS-. The laboratory-scale expression conditions in E.coli BL21 CodonPlus (DE3)-RIL had been optimized and IFN-BLA was expressed higher than 35% of total protein in the cells mainly as inclusion body. The inclusion body of IFN-BLA was denatured and refolded by dialysis and purified by ion-exchange chromatography. The overall yield of IFN-BLA was about 45 mg/L with purity higher than 90%. Antiviral activity assay suggested that this newly fused protein may have synergetic or additive antiviral activities.

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May 2005
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