Short-term changes in prostacyclin secretory profile of irradiated rat cervical spinal cord.

Prostaglandins Leukot Essent Fatty Acids 2005 May;72(5):373-8

Department of Medical Physics, Tehran University of Medical Sciences (TUMS), Tehran, Iran.

Prostaglandins changes in radiation myelopathy (RM) have been previously reported. In the present study, we decided to determine the profile of Prostacyclin (PGI2) content in irradiated rat cervical cord. Wistar rats were irradiated with doses of 2,4,6,15,25 and 30 Gy of X-rays. After 24 h, 2 and 13 weeks post-irradiation, samples of spinal cord were prepared for evaluation of PGI2 and histopathologic changes. Prostacyclin content was determined by quantification of 6-keto-prostaglandin-F1alpha (prostacyclin major metabolite). Irradiated segments of spinal cord were stained routinely for histological studies. Results of irradiated were compared to control groups. Average ratio values of 6-keto-PG-F1alpha for doses of 2-30 Gy were between 67.5% and 107%, 65.41% and 100.54%, and 62.20% and 98.89% for 24 h, 2 and 13 weeks post-irradiation, respectively. Histopathological studies showed marked gliosis and vascularities in irradiated specimens. PGI2 bimodal secretory profile was observed along with histopathological changes in this study. Our results can further emphasize on the role of PGI2 in RM.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.plefa.2005.02.003DOI Listing
May 2005

Publication Analysis

Top Keywords

spinal cord
12
rat cervical
8
secretory profile
8
weeks post-irradiation
8
changes prostacyclin
8
irradiated rat
8
irradiated
6
histological studies
4
stained routinely
4
cord stained
4
studies irradiated
4
routinely histological
4
compared control
4
average ratio
4
ratio values
4
groups average
4
control groups
4
segments spinal
4
irradiated compared
4
prostacyclin major
4

Similar Publications