J Allergy Clin Immunol 2005 Apr;115(4):815-21
Department of Medicine, Division of Pulmonary/Critical Care, Allergy and Clinical Immunology, 96 Jonathan Lucas Street, Charleston, SC 29425, USA.
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J Allergy Clin Immunol 2001 Jun;107(6):1056-62
Department of Medicine, Division of Pulmonary and Critical Care, Medical University of South Carolina, 96 Jonathan Lucas St., Charleston, SC 29425, USA.
Background: Approximately 35% to 40% of patients with chronic urticaria possess a circulating antibody directed to the alpha subunit of the high-affinity type I IgE receptor (FcepsilonRI), which is detectable by using histamine release assays or immunoblotting. Prior reports suggest that purified IgG may not directly activate basophils but rather does so through complement activation.
Objective: We sought to further elucidate the mechanism by which this antibody causes basophil histamine release, including the role of complement, and to reassess the relationship of functional versus binding assays. Read More
J Allergy Clin Immunol 1997 Apr;99(4):461-5
Department of Medicine, State University of New York at Stony Brook 11794-8161, USA.
Background: The etiology of chronic urticaria is unknown, and an exogenous allergen cannot be identified as the cause in the vast majority of subjects. Thus the concept has evolved that it might be autoimmune.
Objective: We have prospectively assessed sera obtained from 50 consecutive patients with chronic urticaria for the presence of autoantibodies that could be pathogenic. Read More
J Allergy Clin Immunol 2002 Jan;109(1):114-8
Department of Medicine, Division of Pulmonary and Critical Care, Allergy and Clinical Immunology, Medical University of South Carolina, Charleston 29425, USA.
Background: Histamine release in chronic urticaria is initiated by cross-linking of the alpha subunit of FcepsilonRI by means of IgG antibody, followed by complement activation.
Objective: We sought to further elucidate the mechanism by which complement augments histamine release and to assess the role of C5a.
Methods: We first quantitated the ability of purified C5a to initiate basophil histamine release and to be inhibited by antibody directed to the C5a receptor. Read More
Br J Dermatol 2004 Aug;151(2):388-96
3rd Department of Medicine, Medical and Health Sciences Centre, University of Debrecen, Hungary.
Background: The autoimmune subclass of chronic idiopathic urticaria (CU) has been characterized by the occurrence of biologically relevant IgG antibodies against the IgE molecule or the alpha chain of the high-affinity Fcepsilon receptor (FcepsilonRIalpha) on basophils and mast cells. These antibodies are usually detected by autologous serum skin testing and confirmed by histamine release studies, immunoblotting, or enzyme-linked immunosorbent assay, but not always.
Objectives: To detect autoantibodies to the FcepsilonRIalpha in sera of CU patients by a modified serum-induced basophil activation test measured by flow cytometry (FCM) and to evaluate the relationship between the in vitro functional test, the autologous serum skin test (ASST), and the serum levels of IgE, eosinophil cationic protein (ECP) and antithyroid antibodies. Read More