Viral genetic determinants for thrips transmission of Tomato spotted wilt virus.

Authors:
Sang-Hoon Sin
Sang-Hoon Sin
Dept. Microbiology & Immunology / University of North Carolina at Chapel Hill
Research Assistant Professor
KSHV
Chapel Hill, NC | United States

Proc Natl Acad Sci U S A 2005 Apr 7;102(14):5168-73. Epub 2005 Mar 7.

Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695, USA.

Tomato spotted wilt virus (TSWV) is transmitted exclusively by thrips in nature. A reassortment-based viral genetic system was used to map transmissibility by thrips to the medium (M) RNA of TSWV. To locate determinants of thrips transmission in the M RNA, 30 single-lesion isolates (SLIs) were generated from a single TSWV isolate that was inefficiently transmitted by thrips. Three of the 30 SLIs were transmitted by thrips, and 27 were not. Sequence analysis of the M RNA, thrips transmissibility assays, G(C) protein analysis, and transmission electron microscopic studies revealed that a specific nonsynonymous mutation (C1375A) in the G(N)/G(C) ORF of the M RNA resulted in the loss of thrips transmissibility without inhibition of virion assembly. This was in contrast to other nontransmissible SLIs, which had frameshift and/or nonsense mutations in the G(N)/G(C) ORF but were defective in virion assembly. The G(C) glycoprotein was detectable in the C1375A mutants but not in the frameshift or nonsense mutants. We report a specific viral determinant associated with virus transmission by thrips. In addition, the loss of transmissibility was associated with the accumulation of defective haplotypes in the population, which are not transmissible by thrips, rather than with the presence of a dominant haplotype that is inefficiently transmitted by thrips. These results also indicate that the glycoproteins may not be required for TSWV infection of plant hosts but are required for transmissibility by thrips.

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.0407354102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC552972PMC
April 2005
5 Reads
9.810 Impact Factor

Publication Analysis

Top Keywords

thrips
12
transmitted thrips
12
virion assembly
8
transmissibility thrips
8
wilt virus
8
thrips transmission
8
tomato spotted
8
inefficiently transmitted
8
viral genetic
8
thrips transmissibility
8
gn/gc orf
8
determinants thrips
8
spotted wilt
8
transmissibility
5
orf rna
4
assembly contrast
4
c1375a gn/gc
4
mutation c1375a
4
rna loss
4
loss thrips
4

References

(Supplied by CrossRef)
Article in Antiviral research
Sidwell et al.
Antiviral research 2003
Article in FIELDS VIROLOGY
FIELDS VIROLOGY 2001
Article in Science
Beaty et al.
Science 1981
Article in PNAS
Beaty et al.
PNAS 1982
Article in Virology
Ludwig et al.
Virology 1989
Article in Virology
Ludwig et al.
Virology 1991
Article in Phytopathology
Kikkert et al.
Phytopathology 1998
Article in Virus research
Medeiros et al.
Virus research 2000
Article in Phytopathology
Bandla et al.
Phytopathology 1998
Article in Molecular plant-microbe interactions : MPMI
Jahn et al.
Molecular plant-microbe interactions : MPMI 2000
Article in Molecular plant-microbe interactions : MPMI
Hoffmann et al.
Molecular plant-microbe interactions : MPMI 2001

Similar Publications