MultiPLX: automatic grouping and evaluation of PCR primers.

Bioinformatics 2005 Apr 14;21(8):1701-2. Epub 2004 Dec 14.

Department of Bioinformatics, University of Tartu, Tartu, Estonia.

Unlabelled: MultiPLX is a new program for automatic grouping of PCR primers. It can use many different parameters to estimate the compatibility of primers, such as primer-primer interactions, primer-product interactions, difference in melting temperatures, difference in product length and the risk of generating alternative products from the template. A unique feature of the MultiPLX is the ability to perform automatic grouping of large number (thousands) of primer pairs.

Availability: Binaries for Windows, Linux and Solaris are available from http://bioinfo.ebc.ee/download/. A graphical version with limited capabilities can be used through a web interface at http://bioinfo.ebc.ee/multiplx/. The source code of the program is available on request for academic users.

Contact: maido.remm@ut.ee.

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Source
http://dx.doi.org/10.1093/bioinformatics/bti219DOI Listing
April 2005
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