MTA obturation of pulpless teeth with open apices: bacterial leakage as detected by polymerase chain reaction assay.

J Endod 2004 Dec;30(12):883-6

Department of Endodontics, School of Dentistry, University of Turin, Italy.

Polymerase chain reaction (PCR) followed by reverse dot blot was used to detect Enterococcus faecalis leakage through mineral trioxide aggregate (MTA) apical obturations of pulpless teeth with open apices. Prepared root canals of 34 extracted teeth were given a standard apical foramen opening and received orthograde apical obturation with MTA; three groups had 1-, 2-, or 3-mm thickness. Sterilized specimens were inoculated with E. faecalis and incubated in sterile medium. DNA extracted from the specimens was amplified by polymerase chain reaction, which yielded a specific segment of E. faecalis 16S rDNA. On day 10 of incubation, no specimens were contaminated. On day 50, almost 17% of specimens were contaminated, with no statistically significant difference between groups (Chi-square = 0.48; df = 2; p = 0.787). Therefore, MTA provides an adequate seal even in cases of orthograde apical obturation of pulpless teeth with open apices.

Download full-text PDF

Source
http://dx.doi.org/10.1097/01.don.0000128749.50151.24DOI Listing
December 2004
6 Reads

Publication Analysis

Top Keywords

pulpless teeth
12
open apices
12
polymerase chain
12
chain reaction
12
teeth open
12
specimens contaminated
8
apical obturation
8
orthograde apical
8
obturation pulpless
8
obturation mta
4
mta
4
received orthograde
4
mta three
4
three groups
4
contaminated day
4
3-mm thickness
4
groups 3-mm
4
day 17%
4
opening received
4
17% specimens
4

Similar Publications