Frequency of -163 C>A and 63 C>G single nucleotide polymorphism of cytochrome P450 1A2 in two African populations.

Clin Chem Lab Med 2004 ;42(8):939-41

Drug Metabolism Group, Department of Biochemistry, University of Zimbabwe, Harare, Zimbabwe.

Cytochrome P450 1A2 (CYP1A2) is an important member of the cytochrome P450 superfamily of enzymes because of its involvement in the metabolism of some carcinogens and therapeutically important drugs. As a result, factors affecting the activity of the enzyme are the focus of considerable research effort as they may have important pharmacological or toxicological implications. CYP1A2 has been shown to exhibit a genetic polymorphism with most of the data, however, coming from studies in Caucasian and Oriental populations. In this study therefore, we investigated the frequencies of two point mutations, -163C>A and 63C>G, in two Bantu African populations. A total of 214 healthy subjects were recruited from Zimbabwe (n=143) and Tanzania (n=71). The two single nucleotide polymorphisms were detected using polymerase chain reaction-restriction fragment length polymorphism analysis. The frequency of -163A was 57% (95% confidence interval (CI), 54%, 60%) and 49% (95% CI, 45%, 53%) among Zimbabweans and Tanzanians, respectively, but the difference between the two populations was not statistically significant (p=0.123). The base change 63 C>G was not found in any of the subjects from the two populations. We report here a high frequency of -163 C>A base change and an absence of the 63 C>G change in the two African populations.

Download full-text PDF

Source
http://dx.doi.org/10.1515/CCLM.2004.152DOI Listing
February 2005
30 Reads
2 Citations
2.960 Impact Factor

Publication Analysis

Top Keywords

cytochrome p450
12
african populations
12
base change
8
p450 1a2
8
single nucleotide
8
-163 c>a
8
frequency -163
8
populations
6
investigated frequencies
4
study investigated
4
95% 45%
4
zimbabweans tanzanians
4
53% zimbabweans
4
frequencies point
4
mutations -163c>a
4
-163c>a 63c>g
4
bantu african
4
45% 53%
4
point mutations
4
63c>g bantu
4

References

(Supplied by CrossRef)
Genetic polymorphism of in Ethiopians affecting induction and expression : characterisation of novel haloptypes with single - nucleotide polymorphisms in intron
Aklillu et al.
Mol Pharmacol 2003
The involvement of and in the metabolism of clozapine
Eiermann et al.
Br J Pharmacol 1997
Interaction between fluvoxamine and cotinine or caffeine
Yoshimura et al.
Neuropsychobiology 2002
Functional significance of a A polymorphism in intron of the cytochrome gene tested with caffeine
Sachse et al.
J Clin Pharmacol 1999
Interindividual variations in human liver cytochrome enzymes involved in the oxidation of drugs , carcinogens , and toxic chemicals : studies with liver microsomes of Japanese and Caucasians
Shimada et al.
J Pharmacol Exp Ther 1994
Genetic polymorphism in the - flanking region of human gene : effect on inducibility in humans
Nakajima et al.
J Biochem 1999
Summary of information on human CYP enzymes : human metabolism data
Rendic et al.
Drug Metab Rev 2002
The effect of the mutation on inducibility in pregnant women
Nordmark et al.
Br J Clin Pharmacol 2002
Detection of a novel cytochrome polymorphism ( in Chinese
Huang et al.
Drug Metab Dispos 1999

Similar Publications