Aggregation of bead-monolayers in flat microfluidic chambers - simulation by the model of porous media.

Authors:
Markus Grumann
Markus Grumann
University of Freiburg
Germany
Michael Dobmeier
Michael Dobmeier
Institute of Organic Chemistry
Patric Schippers
Patric Schippers
University of Freiburg
Germany
Thilo Brenner
Thilo Brenner
IMTEK-University of Freiburg
Freiburg | Germany
Claus Kuhn
Claus Kuhn
BIOmac Research Center
Michael Fritsche
Michael Fritsche
Institute for Biomedical Research
Germany
Roland Zengerle
Roland Zengerle
University of Freiburg
Germany
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Dr. Jens Ducree, Dr. rer. nat. habil. Dipl. Phys.
Fraunhofer Project Centre at Dublin City University
Professor (Full)
microfluidics, Lab-on-a-Chip, hydrodynanmics, business development, project management, organisational leadership
Glasnevin, Dublin 9 | Ireland

Lab Chip 2004 Jun 9;4(3):209-13. Epub 2004 Feb 9.

IMTEK - Institute of Microsystem Technology, Lab for MEMS Applications, University of Freiburg, Georges-Koehler-Allee 103, D-79110 Freiburg, Germany.

In this paper, we for the first time simulate the process of hydrodynamic bead aggregation in a flat micro-fluidic chamber by a porous-media model in an iterative routine. This allows us to optimize the chamber design of our recently developed experimental method to form periodical monolayers from the flow of bead suspension. Periodical monolayers are advantageous for parallel assay formats since they enhance the mechanical rigidity of the aggregated pattern. This is important to avoid a spatial rearrangement along various steps of a read-out procedure which would impair the correlation between measurements. Furthermore, the monolayer formation guarantees the individual optical accessibility of all probe beads. By modelling the monolayers with porous media, we can drastically reduce the degrees of freedom in a two-phase, multi-particle problem. This way, we are able to compute stationary hydrodynamic flow patterns in the chamber. In order to simulate the complete filling process from these stationary solutions, we developed an iterative master routine which takes the transient aggregation pattern as the initial condition, then evaluates the placement of the newly introduced beads, and finally converts the points of aggregation into porous media.

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June 2004
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