A novel role of the hedgehog pathway in lens regeneration.

Dev Biol 2004 Mar;267(2):450-61

Laboratory of Molecular Biology, Department of Biology, University of Dayton, Dayton, OH 45469-2320, USA.

Lens regeneration in the adult newt is a classic example of replacing a lost organ by the process of transdifferentiation. After lens removal, the pigmented epithelial cells of the dorsal iris proliferate and dedifferentiate to form a lens vesicle, which subsequently differentiates to form a new lens. In searching for factors that control this remarkable process, we investigated the expression and role of hedgehog pathway members. These molecules are known to affect retina and pigment epithelium morphogenesis and have been recently shown to be involved in repair processes. Here we show that Shh, Ihh, ptc-1, and ptc-2 are expressed during lens regeneration. The expression of Shh and Ihh is quite unique since these genes have never been detected in lens. Interestingly, both Shh and Ihh are only expressed in the regenerating and developing lens, but not in the intact lens. Interfering with the hedgehog pathway results in considerable inhibition of the process of lens regeneration, including decreased cell proliferation as well as interference with lens fiber differentiation in the regenerating lens vesicle. Down-regulation of ptc-1 was also observed when inhibiting the pathway. These results provide the first evidence of a novel role for the hedgehog pathway in specific regulation of the regenerating lens.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ydbio.2003.12.005DOI Listing
March 2004
45 Reads

Publication Analysis

Top Keywords

lens regeneration
16
hedgehog pathway
16
lens
13
shh ihh
12
role hedgehog
12
form lens
8
lens vesicle
8
regenerating lens
8
novel role
8
pathway
5
expression shh
4
ihh unique
4
unique genes
4
regeneration expression
4
expression role
4
genes detected
4
expressed regenerating
4
regenerating developing
4
ihh expressed
4
interestingly shh
4

Similar Publications