Micro- and nanofluidics for DNA analysis.

Anal Bioanal Chem 2004 Apr 5;378(7):1678-92. Epub 2004 Mar 5.

Department of Molecular Biology, Princeton University, Princeton, NJ, USA.

Miniaturization to the micrometer and nanometer scale opens up the possibility to probe biology on a length scale where fundamental biological processes take place, such as the epigenetic and genetic control of single cells. To study single cells the necessary devices need to be integrated on a single chip; and, to access the relevant length scales, the devices need to be designed with feature sizes of a few nanometers up to several micrometers. We will give a few examples from the literature and from our own research in the field of miniaturized chip-based devices for DNA analysis, including dielectrophoresis for purification of DNA, artificial gel structures for rapid DNA separation, and nanofluidic channels for direct visualization of single DNA molecules.

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http://dx.doi.org/10.1007/s00216-004-2526-0DOI Listing
April 2004
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