Biochem Biophys Res Commun 2021 Feb 22;548:47-52. Epub 2021 Feb 22.
Faculty of Materials Science and Engineering, South China University of Technology, Guangzhou, 510640, PR China.
The mechanism for protein stabilization or destabilization has long been an open quest. In the present study, we have studied the interactions between amino acids and guanidinium (Gdm)/ammonium (NH) ions by using low field nuclear magnetic resonance (LF-NMR), where Gdm and NH are denaturant and stabilizer for proteins, respectively. It shows that Gdm favors to bind to the thiol group or the hydroxyl group on the side chain but weakly interacts with the α-carboxyl group. Read More