Unnatural amino acid mutagenesis in mapping ion channel function.

Curr Opin Neurobiol 2003 Jun;13(3):264-70

Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.

Unnatural amino acid mutagenesis makes possible the site-specific incorporation of synthetic amino acids, enabling detailed structure-function studies as well as the incorporation of biophysical probes. This method has been adapted for use with heterologous expression in Xenopus oocytes, allowing experiments on ion channels.

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http://dx.doi.org/10.1016/s0959-4388(03)00068-0DOI Listing
June 2003
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