Pubfacts - Scientific Publication Data
  • Categories
  • |
  • Journals
  • |
  • Authors
  • Login
  • Categories
  • Journals

Search Our Scientific Publications & Authors

Publications
  • Publications
  • Authors
find publications by category +
Translate page:

Mycobacterium bovis BCG response regulator essential for hypoxic dormancy.

Authors:
Calvin Boon Thomas Dick

J Bacteriol 2002 Dec;184(24):6760-7

Mycobacterium Biology Laboratory, Institute of Molecular and Cell Biology, Singapore 117609, Republic of Singapore.

Obligately aerobic tubercle bacilli are capable of adapting to survive hypoxia by developing into a nonreplicating or dormant form. Dormant bacilli maintain viability for extended periods. Furthermore, they are resistant to antimycobacterials, and hence, dormancy might play a role in the persistence of tuberculosis infection despite prolonged chemotherapy. Previously, we have grown dormant Mycobacterium bovis BCG in an oxygen-limited Wayne culture system and subjected the bacilli to proteome analysis. This work revealed the upregulation of the response regulator Rv3133c and three other polypeptides (alpha-crystallin and two "conserved hypothetical" proteins) upon entry into dormancy. Here, we replaced the coding sequence of the response regulator with a kanamycin resistance cassette and demonstrated that the loss-of-function mutant died after oxygen starvation-induced termination of growth. Thus, the disruption of this dormancy-induced transcription factor resulted in loss of the ability of BCG to adapt to survival of hypoxia. Two-dimensional gel electrophoresis of protein extracts from the gene-disrupted strain showed that the genetic loss of the response regulator caused loss of the induction of the other three dormancy proteins. Thus, the upregulation of these dormancy proteins requires the response regulator. Based on these two functions, dormancy survival and regulation, we named the Rv3133c gene dosR for dormancy survival regulator. Our results provide conclusive evidence that DosR is a key regulator in the oxygen starvation-induced mycobacterial dormancy response.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC135468PMC
http://dx.doi.org/10.1128/jb.184.24.6760-6767.2002DOI Listing
December 2002

Publication Analysis

Top Keywords

response regulator
20
dormancy proteins
8
dormancy
8
oxygen starvation-induced
8
mycobacterium bovis
8
dormancy survival
8
bovis bcg
8
regulator
7
response
6
cassette demonstrated
4
starvation-induced termination
4
died oxygen
4
mutant died
4
termination growth
4
demonstrated loss-of-function
4
loss-of-function mutant
4
transcription factor
4
ability bcg
4
bcg adapt
4
adapt survival
4

Similar Publications

Molecular characterisation of titin N2A and its binding of CARP reveals a titin/actin cross-linking mechanism.

Authors:
Tiankun Zhou Jennifer R Fleming Stephan Lange Anthony L Hessel Julius Bogomolovas Chiara Stronczek David Grundei Majid Ghassemian Andrea Biju Emma Börgeson Belinda Bullard Wolfgang A Linke Ju Chen Michael Kovermann Olga Mayans

J Mol Biol 2021 Feb 26:166901. Epub 2021 Feb 26.

Department of Biology, University of Konstanz, 78457 Konstanz, Germany. Electronic address:

Striated muscle responds to mechanical overload by rapidly up-regulating the expression of the cardiac ankyrin repeat protein, CARP, which then targets the sarcomere by binding to titin N2A in the I-band region. To date, the role of this interaction in the stress response of muscle remainspoorly understood. Here, we characterise the molecular structure of the CARP-receptor site in titin (UN2A) and its binding of CARP. Read More

View Article and Full-Text PDF
February 2021
Similar Publications

Diversified Regulation of Cytokinin Levels and Signaling During Infection in .

Authors:
Beibei Li Ruolin Wang Shiya Wang Jiang Zhang Ling Chang

Front Plant Sci 2021 10;12:584042. Epub 2021 Feb 10.

State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.

Cytokinins (CKs) can modulate plant immunity to various pathogens, but how CKs are involved in plant defense responses to the necrotrophic pathogen is still unknown. Here, we found that infection induced transcriptional changes in multiple genes involved in the biosynthesis, degradation, and signaling of CKs, as well as their contents, in pathogen-infected leaves. Among the CKs, the gene expression of () was remarkably induced in the local infected leaves and the distant leaves of the same plant without pathogen inoculation. Read More

View Article and Full-Text PDF
February 2021
Similar Publications

The Alternative Sigma Factor RpoE2 Is Involved in the Stress Response to Hypochlorite and Survival of .

Authors:
Marufa Nasreen Aidan Fletcher Jennifer Hosmer Qifeng Zhong Ama-Tawiah Essilfie Alastair G McEwan Ulrike Kappler

Front Microbiol 2021 12;12:637213. Epub 2021 Feb 12.

Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD, Australia.

Extracytoplasmic function (ECF) sigma factors underpin the ability of bacteria to adapt to changing environmental conditions, a process that is particularly relevant in human pathogens that inhabit niches where human immune cells contribute to high levels of extracellular stress. Here, we have characterized the previously unstudied RpoE2 ECF sigma factor from the human respiratory pathogen (Hi) and its role in hypochlorite-induced stress. Exposure of to oxidative stress (HOCl, HO) increased gene expression, and the activity of RpoE2 was controlled by a cytoplasmic 67-aa anti-sigma factor, HrsE. Read More

View Article and Full-Text PDF
February 2021
Similar Publications

The role of the gut microbiota on the metabolic status of obese children.

Authors:
Xin Yuan Ruimin Chen Kenneth L McCormick Ying Zhang Xiangquan Lin Xiaohong Yang

Microb Cell Fact 2021 Feb 27;20(1):53. Epub 2021 Feb 27.

Department of Endocrinology, Fuzhou Children's Hospital of Fujian Medical University, NO. 145, 817 Middle Road, Fuzhou, 350005, China.

Background: The term "metabolically healthy obese (MHO)" denotes a hale and salutary status, yet this connotation has not been validated in children, and may, in fact, be a misnomer. As pertains to obesity, the gut microbiota has garnered attention as conceivably a nosogenic or, on the other hand, protective participator.

Objective: This study explored the characteristics of the fecal microbiota of obese Chinese children and adolescents of disparate metabolic statuses, and the associations between their gut microbiota and circulating proinflammatory factors, such as IL-6, TNF-α, lipopolysaccharide-binding protein (LBP), and a cytokine up-regulator and mediator, leptin. Read More

View Article and Full-Text PDF
February 2021
Similar Publications

Hydrogen Gas Therapy Attenuates Inflammatory Pathway Signaling in Septic Mice.

Authors:
Hiroshi Matsuura Hisatake Matsumoto Daisuke Okuzaki Kentaro Shimizu Hiroshi Ogura Takeshi Ebihara Tsunehiro Matsubara Shin-Ichi Hirano Takeshi Shimazu

J Surg Res 2021 Feb 24;263:63-70. Epub 2021 Feb 24.

Department of Traumatology and Acute Critical Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.

Background: Molecular hydrogen (H) has been used in clinical cases. However, there are few studies of H therapy to treat sepsis, and anti-inflammatory mechanisms of H are mostly unknown. We aimed to confirm effects of H therapy on sepsis and reveal its therapeutic mechanism via RNA sequencing in multiple organs in septic mice. Read More

View Article and Full-Text PDF
February 2021
Similar Publications
© 2021 PubFacts.
  • About PubFacts
  • Privacy Policy
  • Sitemap