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J Gen Physiol 2021 Jul;153(7)

Department of Biology, University of Konstanz, Konstanz, Germany.

The N2A segment of titin is a main signaling hub in the sarcomeric I-band that recruits various signaling factors and processing enzymes. It has also been proposed to play a role in force production through its Ca2+-regulated association with actin. However, the molecular basis by which N2A performs these functions selectively within the repetitive and extensive titin chain remains poorly understood. Read More

Physiol Int 2021 Apr 8. Epub 2021 Apr 8.

1Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.

Aging is a multifactorial process, which is considered as a decline over time. It is increasingly clear that there is a gender difference in aging and in the prevalence of age-related diseases as well. We aimed to examine the effects of the aging process in the colonic tissue of female Wistar rats aged 10 weeks (young) and 13 months (middle-aged) at an early stage, according to three main symptoms associated with aging: a decrease in the efficacy of the proteasome and muscle function and an increase in oxidative stress. Read More

Environ Sci Pollut Res Int 2021 Apr 8. Epub 2021 Apr 8.

Hebei Key Lab of Power Plant Flue Gas Multi-Pollutants Control, Department of Environmental Science and Engineering, North China Electric Power University, Baoding, 071003, People's Republic of China.

Selenium (Se) is one of the toxic trace elements in flue gas emitted from coal-fired power plants. A method for oxidation and absorption of selenium dioxide (SeO) by hydrogen peroxide (HO) and sodium chlorite (NaClO) in a calcium-based slurry is proposed, in which hydrated lime (Ca(OH)) and calcium carbonate (CaCO) were selected to be absorbents, and HO and NaClO were used to oxidize SeO and selenite ion to selenate ion to reduce their toxicities. The effects of reaction temperature, HO and NaClO concentrations, solution pH, and SeO concentration were investigated, from which the optimal reaction conditions were established. Read More

Biochemistry (Mosc) 2021 Feb;86(2):123-131

Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, 119334, Russia.

Here, we propose a new approach for quantitative estimation of von Willebrand factor (vWF) exposed on the surface of endothelial cells (ECs) using the ARC1779 aptamer that interacts with the vWF A1 domain. To visualize complex formation between vWF and the aptamer, the latter was conjugated with the Cy5 fluorescent label. Cultured human umbilical vein endothelial cells (HUVEC) were stained with the ARC1779-Cy5 conjugate and imaged with a fluorescence microscope. Read More

Cell Calcium 2021 Mar 27;96:102404. Epub 2021 Mar 27.

Applied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama 700-8530, Japan. Electronic address:

To elucidate S100 protein-mediated signaling pathways, we attempted to identify novel binding partners for S100A2 by screening protein arrays carrying 19,676 recombinant glutathione S-transferase (GST)-fused human proteins with biotinylated S100A2. Among newly discovered putative S100A2 interactants, including TMLHE, TRH, RPL36, MRPS34, CDR2L, OIP5, and MED29, we identified and characterized the tubulin polymerization-promoting protein (TPPP) as a novel S100A2-binding protein. We confirmed the interaction of TPPP with Ca/S100A2 by multiple independent methods, including the protein array method, S100A2 overlay, and pulldown assay in vitro and in transfected COS-7 cells. Read More