Inhibition of CD40 expression and CD40-mediated dendritic cell function by tumor-derived IL-10.

Int J Cancer 2002 Sep;101(1):61-8

Department of Pathology, University of Pittsburgh Medical Center, PA 15213, USA.

As CD40 plays a key role in both antitumor immunity and DC maturation, we have studied the regulation of its expression during DC hematopoiesis (dendropoiesis) in vitro and in vivo in the tumor microenvironment. Using MC38 colon adenocarcinoma tumor models, we have demonstrated that DCs generated in vitro from bone marrow precursors obtained from tumor-bearers have significantly lower expression of CD40 molecules compared to DCs generated from tumor-free mice. Furthermore, CD40 expression on DCs isolated from the spleens of tumor-bearing mice was also significantly reduced, suggesting that tumor-derived factors inhibit CD40 expression on DCs during dendropoiesis both in vitro and in vivo. Interestingly, CD40 ligation on DCs generated from tumor-bearers did not result in inducible expression of IL-12 protein or IL-12 p40 mRNA. However, Staphylococcus aureus-induced IL-12 production by DCs was not altered in tumor-bearers, confirming that inhibition of IL-12 production by DCs generated in vitro from tumor-bearing mice was due to reduced expression of CD40 on DCs. We have also shown that MC38 tumor cells produce IL-10 and that exogenous IL-10 causes downregulation of CD40 expression on DCs. In addition, endogenous IL-10 produced by colon carcinoma cells inhibited CD40-dependent IL-12 production by DCs since tumor-induced inhibition of IL-12 production was abrogated by neutralizing anti-IL-10 antibody. Finally, systemic administration of FLT3L and/or CD40L reversed CD40 and IL-12 (p40) deficiency of DCs in tumor-bearing mice in vivo. These findings thus demonstrate that tumor-derived factors, including IL-10, inhibit CD40 expression on DCs and DC precursors and suppress their maturation and function.

Download full-text PDF

Source
http://dx.doi.org/10.1002/ijc.10576DOI Listing
September 2002
1 Read

Publication Analysis

Top Keywords

cd40 expression
20
il-12 production
16
dcs generated
16
expression dcs
16
production dcs
12
dcs
12
tumor-bearing mice
12
cd40
9
expression
9
il-12 p40
8
dendropoiesis vitro
8
mice reduced
8
vitro vivo
8
inhibition il-12
8
generated vitro
8
expression cd40
8
tumor-derived factors
8
inhibit cd40
8
il-12
7
il-10
5

References

(Supplied by CrossRef)
Immunologic dysfunction in cancer
Kavanaugh et al.
Hematol Oncol Clin North Am 1996
Dendritic cells presenting tumor antigen
Shurin et al.
Cancer Immunol Immunother 1996
Regulation of the dendritic cell system by tumor
Shurin et al.
Cancer Res Ther Control 2001
Immunophenotype analysis of dendritic cells and lymphocytes associated with cutaneous malignant melanomas
Garcia-Plata et al.
Invasion Metastasis 1995
Decreased antigen presentation by dendritic cells in patients with breast cancer
Gabrilovich et al.
Clin Cancer Res 1997
Human sunlight-induced basal-cell-carcinoma-associated dendritic cells are deficient in T cell co-stimulatory molecules and are impaired as antigen-presenting cells
Nestle et al.
Am J Pathol 1997
Tumor's other immune targets: dendritic cells
Esche et al.
J Leukoc Biol 1999

Similar Publications