Interleukin-10 inhibits expression of interleukin-6 and -8 mRNA in human dental pulp cell cultures via nuclear factor-kappaB deactivation.

J Endod 2002 Mar;28(3):177-80

Department of Operative Dentistry and Endodontology, Kagoshima University Dental School, Japan.

The effects of interleukin (IL)-10 on the expression of IL-6 and IL-8 mRNA in human dental pulp cell cultures were investigated by using the Northern blot analysis. On stimulation with Prevotella intermedia lipopolysaccharide (PiLPS), IL-10 was produced in peripheral blood but was not detected in human dental pulp cell culture supernatants. IL-10 inhibited IL-8 mRNA expression, which is normally stimulated by PiLPS, IL-1alpha, and tumor necrosis factor-alpha and inhibited IL-6 mRNA expression, which is normally stimulated by IL-1alpha. In addition, IL-10 inhibited the activation of nuclear factor-kappaB, which is normally induced by PiLPS. We conclude that IL-10 inhibits expression of IL-6 and IL-8 mRNA in dental pulp cell cultures by inhibiting the activation of nuclear factor-kappaB.

Download full-text PDF

Source
http://dx.doi.org/10.1097/00004770-200203000-00008DOI Listing
March 2002
5 Reads

Publication Analysis

Top Keywords

dental pulp
16
pulp cell
16
human dental
12
il-8 mrna
12
cell cultures
12
nuclear factor-kappab
12
expression il-6
8
il-10 inhibited
8
mrna expression
8
expression stimulated
8
activation nuclear
8
il-6 il-8
8
mrna human
8
inhibits expression
8
expression
5
mrna
5
il-10
5
tumor necrosis
4
intermedia lipopolysaccharide
4
il-1alpha tumor
4

Similar Publications