Dr. Reza Rahbarghazi, DVM, PhD - Stem Cell Research Center , Tabriz University of Medical Sciences, Tabriz, Iran - Dr. Reza Rahbarghazi

Dr. Reza Rahbarghazi

DVM, PhD

Stem Cell Research Center , Tabriz University of Medical Sciences, Tabriz, Iran

Dr. Reza Rahbarghazi

Tabriz, East Azerbaijan Province | Saint Kitts and Nevis

Main Specialties: Cardiovascular Disease

Additional Specialties: Stem Cell, Angiogenesis, Cardiology

ORCID logohttps://orcid.org/0000-0003-3864-9166

Dr. Reza Rahbarghazi, DVM, PhD - Stem Cell Research Center , Tabriz University of Medical Sciences, Tabriz, Iran - Dr. Reza Rahbarghazi

Dr. Reza Rahbarghazi

DVM, PhD

Introduction

Primary Affiliation: Stem Cell Research Center , Tabriz University of Medical Sciences, Tabriz, Iran - Tabriz, East Azerbaijan Province , Saint Kitts and Nevis

Specialties:

Additional Specialties:

Publications

75Publications

904Reads

1245Profile Views

78PubMed Central Citations

Distinct Tie2 tyrosine phosphorylation sites dictate phenotypic switching in endothelial progenitor cells.

J Cell Physiol 2019 May 24;234(5):6209-6219. Epub 2018 Sep 24.

Department of Pharmacology, Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran.

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/jcp.27349
Publisher Site
http://dx.doi.org/10.1002/jcp.27349DOI Listing
May 2019
3 Reads
3.840 Impact Factor

Quercetin alleviates high glucose-induced damage on human umbilical vein endothelial cells by promoting autophagy.

Phytomedicine 2019 Mar 8;56:183-193. Epub 2018 Nov 8.

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S09447113183056
Publisher Site
http://dx.doi.org/10.1016/j.phymed.2018.11.008DOI Listing
March 2019
14 Reads
3.126 Impact Factor

Curcumin modulates the angiogenic potential of human endothelial cells via FAK/P-38 MAPK signaling pathway.

Gene 2019 Mar 23;688:7-12. Epub 2018 Nov 23.

Neurophysiology Research Center, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S03781119183120
Publisher Site
http://dx.doi.org/10.1016/j.gene.2018.11.062DOI Listing
March 2019
36 Reads
2.138 Impact Factor

Encapsulation of rat cardiomyoblasts with alginate-gelatin microspheres preserves stemness feature in vitro.

Biomed Pharmacother 2019 Jan 3;109:402-407. Epub 2018 Nov 3.

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S07533322183583
Publisher Site
http://dx.doi.org/10.1016/j.biopha.2018.10.119DOI Listing
January 2019
22 Reads
2.023 Impact Factor

Resveratrol potentially increased the tumoricidal effect of doxorubicin on SKOV3 cancer stem cells in vitro.

J Cell Biochem 2019 Jan 4. Epub 2019 Jan 4.

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/jcb.28129
Publisher Site
http://dx.doi.org/10.1002/jcb.28129DOI Listing
January 2019
13 Reads
3.263 Impact Factor

Bone marrow mesenchymal stem cells and condition media diminish inflammatory adhesion molecules of pulmonary endothelial cells in an ovalbumin-induced asthmatic rat model.

Microvasc Res 2019 01 18;121:63-70. Epub 2018 Oct 18.

Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00262862183012
Publisher Site
http://dx.doi.org/10.1016/j.mvr.2018.10.005DOI Listing
January 2019
19 Reads
2.130 Impact Factor

Photo-modulation of zinc phthalocyanine-treated breast cancer cell line ZR-75-1 inhibited the normal tumor activity in vitro.

Lasers Med Sci 2018 Dec 24;33(9):1969-1978. Epub 2018 Aug 24.

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10103-018-2563-0DOI Listing
December 2018
3 Reads
2.490 Impact Factor

Systemic Transplantation of Mesenchymal Stem Cells Modulates Endothelial Cell Adhesion Molecules Induced by Ovalbumin in Rat Model of Asthma.

Inflammation 2018 Dec;41(6):2236-2245

Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10753-018-0866-8DOI Listing
December 2018
20 Reads
1.921 Impact Factor

Prolonged incubation with Metformin decreased angiogenic potential in human bone marrow mesenchymal stem cells.

Biomed Pharmacother 2018 Dec 4;108:1328-1337. Epub 2018 Oct 4.

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S07533322183394
Publisher Site
http://dx.doi.org/10.1016/j.biopha.2018.09.135DOI Listing
December 2018
15 Reads
2.023 Impact Factor

Diabetic sera disrupted the normal exosome signaling pathway in human mesenchymal stem cells in vitro.

Cell Tissue Res 2018 Dec 2;374(3):555-565. Epub 2018 Aug 2.

Department of Biology, Faculty of Sciences, Arak University, Arak, 3815688349, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00441-018-2895-xDOI Listing
December 2018
2 Reads
3.565 Impact Factor

Systemic delivery of mesenchymal stem cells condition media in repeated doses acts as magic bullets in restoring IFN-γ/IL-4 balance in asthmatic rats.

Life Sci 2018 Nov 27;212:30-36. Epub 2018 Sep 27.

Tuberculosis and lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2018.09.049DOI Listing
November 2018
6 Reads
2.702 Impact Factor

Silibinin protects human endothelial cells from high glucose-induced injury by enhancing autophagic response.

J Cell Biochem 2018 Nov 26;119(10):8084-8094. Epub 2018 Jun 26.

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcb.26735DOI Listing
November 2018
4 Reads
3.263 Impact Factor

Distinct role of autophagy on angiogenesis: highlights on the effect of autophagy in endothelial lineage and progenitor cells.

Stem Cell Res Ther 2018 11 8;9(1):305. Epub 2018 Nov 8.

Department of Clinical Biochemistry and Laboratory Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13287-018-1060-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6225658PMC
November 2018
2 Reads
3.368 Impact Factor

High glucose condition limited the angiogenic/cardiogenic capacity of murine cardiac progenitor cells in in vitro and in vivo milieu.

Cell Biochem Funct 2018 Oct 26;36(7):346-356. Epub 2018 Jul 26.

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/cbf.3354DOI Listing
October 2018
2 Reads
2.005 Impact Factor

Cancer stem cells-emanated therapy resistance: Implications for liposomal drug delivery systems.

J Control Release 2018 Oct 2;288:62-83. Epub 2018 Sep 2.

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S01683659183051
Publisher Site
http://dx.doi.org/10.1016/j.jconrel.2018.08.043DOI Listing
October 2018
87 Reads
7.705 Impact Factor

Heat shock protein 70 modulates neural progenitor cells dynamics in human neuroblastoma SH-SY5Y cells exposed to high glucose content.

J Cell Biochem 2018 08 18;119(8):6482-6491. Epub 2018 Apr 18.

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/jcb.26679
Publisher Site
http://dx.doi.org/10.1002/jcb.26679DOI Listing
August 2018
17 Reads
3.263 Impact Factor

Synthesis and in vitro evaluation of thermosensitive hydrogel scaffolds based on (PNIPAAm-PCL-PEG-PCL-PNIPAAm)/Gelatin and (PCL-PEG-PCL)/Gelatin for use in cartilage tissue engineering.

J Biomater Sci Polym Ed 2018 07 5;29(10):1185-1206. Epub 2018 Mar 5.

a Department of Chemistry , University of Zanjan , Zanjan , Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/09205063.2018.1447627DOI Listing
July 2018
5 Reads
2 Citations
1.650 Impact Factor

Docosahexaenoic acid reversed atherosclerotic changes in human endothelial cells induced by palmitic acid in vitro.

Cell Biochem Funct 2018 Jun 13;36(4):203-211. Epub 2018 Apr 13.

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/cbf.3332DOI Listing
June 2018
7 Reads
2.005 Impact Factor

The Dynamics of Neurosteroids and Sex-Related Hormones in the Pathogenesis of Alzheimer's Disease.

Neuromolecular Med 2018 Jun 4;20(2):215-224. Epub 2018 May 4.

Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., Tabriz, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12017-018-8493-yDOI Listing
June 2018
4 Reads
3.680 Impact Factor

Bone marrow mesenchymal stem cells modified pathological changes and immunological responses in ovalbumin-induced asthmatic rats possibly by the modulation of miRNA155 and miRNA133.

Gen Physiol Biophys 2018 May;37(3):263-274

Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.4149/gpb_2017052DOI Listing
May 2018
3 Reads
1.173 Impact Factor

Exosomes and their Application in Biomedical Field: Difficulties and Advantages.

Mol Neurobiol 2018 Apr 11;55(4):3372-3393. Epub 2017 May 11.

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12035-017-0582-7DOI Listing
April 2018
29 Reads
4 Citations
5.140 Impact Factor

Label-free biosensors in the field of stem cell biology.

Biosens Bioelectron 2018 Mar 16;101:188-198. Epub 2017 Oct 16.

Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran; Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2017.10.028DOI Listing
March 2018
7 Reads
1 Citation
6.410 Impact Factor

Distinct effect of fetal bovine serum versus follicular fluid on multipotentiality of human granulosa cells in in vitro condition.

Biologicals 2018 Mar 3;52:44-48. Epub 2018 Feb 3.

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biologicals.2018.01.002DOI Listing
March 2018
6 Reads
1.210 Impact Factor

Angiogenic and Restorative Abilities of Human Mesenchymal Stem Cells Were Reduced Following Treatment With Serum From Diabetes Mellitus Type 2 Patients.

J Cell Biochem 2018 01 14;119(1):524-535. Epub 2017 Jul 14.

Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcb.26211DOI Listing
January 2018
17 Reads
1 Citation
3.263 Impact Factor

Distinct Effects of Royal Jelly on Human Endothelial Cells Under High Glucose Condition.

Iran J Pharm Res 2018 ;17(4):1361-1370

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269585PMC
January 2018
1 Read

Stem Cells as a Promising Tool for the Restoration of Brain Neurovascular Unit and Angiogenic Orientation.

Mol Neurobiol 2017 12 14;54(10):7689-7705. Epub 2016 Nov 14.

Neuroscience Research Center, Imam Reza Medical Center, Tabriz University of Medical Sciences, Golgasht St., Azadi Ave, Tabriz, 5166614756, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12035-016-0286-4DOI Listing
December 2017
18 Reads
5.140 Impact Factor

Early-stage detection of VE-cadherin during endothelial differentiation of human mesenchymal stem cells using SPR biosensor.

Biosens Bioelectron. 2017 Oct 15;96:358-366

Biosensors and Bioelectronics

Surface plasmon resonance (SPR) biosensors are most commonly applied for real-time dynamic analysis and measurement of interactions in bio-molecular studies and cell-surface analysis without the need for labeling processes. Up to present, SPR application in stem cell biology and biomedical sciences was underused. Herein, a very simple and sensitive method was developed to evaluate human mesenchymal stem cells trans-differentiation to endothelial lineage of over a period of 14 days based on VE-cadherin biomarker. The SPR signals increased with the increase of the amount of VE-cadherin expression on the cell surface during cell differentiation process. The method was able to detect ≈27 cells permm2. No significant effect was observed on the cell viability during the cell attachment to the surface of immune-reactive biochips and during the SPR analysis. Using this highly sensitive SPR method, it was possible to sense the early stage of endothelial differentiation on day 3 in label-free form, whereas flow cytometry and fluorescent microscopy methods were found unable to detect the cell differentiation at the same time. Therefore, the proposed method can rapidly and accurately detect cell differentiation in live cells and label-free manner without any need of cell breakage and has great potential for both diagnostic and experimental approaches.

View Article
October 2017
4 Reads

Type 2 Diabetes Inhibited Human Mesenchymal Stem Cells Angiogenic Response by Over-Activity of the Autophagic Pathway.

J Cell Biochem 2017 06 11;118(6):1518-1530. Epub 2017 Jan 11.

Faculty of Pharmacy, Department of Pharmacology and Toxicology, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcb.25814DOI Listing
June 2017
17 Reads
3 Citations
3.263 Impact Factor

Rat adipose-derived mesenchymal stem cells aging reduction by zinc sulfate under extremely low frequency electromagnetic field exposure is associated with increased telomerase reverse transcriptase gene expression

Vet Res Forum. 2017 Spring; 8(2):89-96

Veterinary Research Forum

Zinc as an essential trace element was reported to be involved in regulation of the growth and aging of cells. In this study, rat adipose-derived mesenchymal stem cells were exposed to extremely low frequency electromagnetic field (ELF-EMF) of 50 Hz and 20 mT to evaluate whether exposure to ELF-EMF in the presence of zinc sulfate (ZnSO4) affects the telomerase reverse transcriptase (TERT) gene expression and aging in mesenchymal stem cells (MSCs). The cell plates were divided into four groups including group I (control without ZnSO4 and ELF-EMF exposure); group II (ELF-EMF-exposure without ZnSO4); group III (ZnSO4 treatment without ELF-EMF exposure) and group ІV (ELF-EMF exposure with ZnSO4). In the presence of different concentrations of ZnSO4,cells viability, TERT gene expression and percentage of senescent cells were evaluated using colorimetric assay, real-time PCR and senescence-associated β-galactosidase activity assay, respectively. In this experiment, cells were exposed to ELF-EMF for 30 min per day for 21 days in the presence and absence of ZnSO4. The results revealed that ELF-EMF leads to a decrease in the expression of TERT gene and increase in the percentage of senescent cells. However, the ZnSO4 could significantly increase the TERT gene expression and decrease the aging of ELF-EMF-exposed MSCs. It seems that ZnSO4 may be a beneficial agent to delay aging of ELF-EMF-exposed MSCs due to the induction of TERT gene expression. Zinc as an essential trace element was reported to be involved in regulation of the growth and aging of cells. In this study, rat adipose-derived mesenchymal stem cells were exposed to extremely low frequency electromagnetic field (ELF-EMF) of 50 Hz and 20 mT to evaluate whether exposure to ELF-EMF in the presence of zinc sulfate (ZnSO4) affects the telomerase reverse transcriptase (TERT) gene expression and aging in mesenchymal stem cells (MSCs). The cell plates were divided into four groups including group I (control without ZnSO4 and ELF-EMF exposure); group II (ELF-EMF-exposure without ZnSO4); group III (ZnSO4 treatment without ELF-EMF exposure) and group ІV (ELF-EMF exposure with ZnSO4). In the presence of different concentrations of ZnSO4,cells viability, TERT gene expression and percentage of senescent cells were evaluated using colorimetric assay, real-time PCR and senescence-associated β-galactosidase activity assay, respectively. In this experiment, cells were exposed to ELF-EMF for 30 min per day for 21 days in the presence and absence of ZnSO4. The results revealed that ELF-EMF leads to a decrease in the expression of TERT gene and increase in the percentage of senescent cells. However, the ZnSO4 could significantly increase the TERT gene expression and decrease the aging of ELF-EMF-exposed MSCs. It seems that ZnSO4 may be a beneficial agent to delay aging of ELF-EMF-exposed MSCs due to the induction of TERT gene expression.

View Article
June 2017
3 Reads

ANGIOGENIC AND RESTORATIVE ABILITIES OF HUMAN MESENCHYMAL STEM CELLS WERE REDUCED FOLLOWING TREATMENT WITH SERUM FROM DIABETES MELLITUS TYPE 2 PATIENTS.

J Cell Biochem. 2017 Jun 13.

J Cell Biochem

This experiment investigated the impact of serum from patients with type 2 diabetes mellitus on the angiogenic behavior of human mesenchymal stem cells in vitro. Changes in the level of Ang-1, Ang-2, cell migration and trans-differentiation into pericytes and endothelial lineage were monitored after 7 days. The interaction of mesenchymal stem cells with endothelial cells were evaluated using surface plasmon resonance technique. Paracrine restorative effect of diabetic stem cells was tested on pancreatic β cells. Compared to data from FBS and normal serum, diabetic serum reduced the stem cell survival and chemotaxis toward VEGF and SDF-1α (p < 0.05). Diabetic condition were found to decline cell migration rate and the activity of MMP-2 and -9 (p < 0.05). The down-regulation of VEGFR-2 and CXCR-4 was observed with an increase in the level of miR-1-3p and miR-15b-5p at the same time. The paracrine angiogenic potential of diabetic stem cells was disturbed via the changes in the dynamic of Ang-1, Ang-2, and VEGF. Surface plasmon resonance analysis showed that diabetes could induce an aberrant increase in the interaction of stem cells with endothelial cells. After treatment with diabetic serum, the expression of VE-cadherin and NG2 and ability for uptake of Dil-Ac-LDL were reduced (p < 0.01). Conditioned media prepared from diabetic stem cells were unable to decrease fatty acid accumulation in β-cells (p < 0.05). The level of insulin secreted by β-cells was not affected after exposure to supernatant from diabetic or non-diabetic mesenchymal stem cells. Data suggest diabetes could decrease angiogenic and restorative effect of stem cells in vitro. This article is protected by copyright. All rights reserved.

View Article
June 2017
4 Reads

Endothelial cells' biophysical, biochemical, and chromosomal aberrancies in high-glucose condition within the diabetic range.

Cell Biochem Funct. 2017 Mar;35(2):83-97.

Cell Biochem Funct.

To date, many studies have been conducted to find out the underlying mechanisms of hyperglycemia-induced complications in diabetes mellitus, attributed to the cellular pathologies of different cells-especially endothelial cells. However, there are still many ambiguities and unresolved issues to be clarified. Here, we investigated the alteration in biophysical and biochemical properties in human umbilical vein endothelial cells exposed to a high-glucose concentration (30mM), comparable to glucose content in type 2 diabetes mellitus, over a course of 120 hours. In addition to a reduction in the rate of cell viability and induction of oxidative stress orchestrated by the high-glucose condition, the dynamic of the fatty acid profile-including polyunsaturated, monounsaturated, and saturated fatty acids-was also altered in favor of saturated fatty acids. Genetic imbalances were also detected at chromosomal level in the cells exposed to the abnormal concentration of glucose after 120 hours. Moreover, the number of tip cells (CD31+ /CD34+ ) and in vitro tubulogenesis capability negatively diminished in comparison to parallel control groups. We found that diabetic hyperglycemia was associated with a decrease in the cell-cell tight junction and upregulation in vascular endothelial cadherin and zonula occludens (ZO)-1 molecules after 72 and 120 hours of exposure to the abnormal glucose concentration, which resulted in a profound reduction in transendothelial electrical resistance. The surface plasmon resonance analysis of the human umbilical vein endothelial cells immobilized on gold-coated sensor chips confirmed the loosening of the cell to cell intercellular junction as well as stable attachment of each cell to the basal surface. Our findings highlighted the disturbing effects of a diabetic hyperglycemia on either biochemical or biophysical properties of endothelial cells.

View Article
May 2017
4 Reads

Copper sulfate pentahydrate reduced epithelial cytotoxicity induced by lipopolysaccharide from enterogenic bacteria.

Biomed Pharmacother. 2017 May;89:454-461.

Biomed Pharmacother

The over usage of multiple antibiotics contributes to the emergence of a whole range of antibiotic-resistant strains of bacteria causing enterogenic infections in poultry science. Therefore, finding an appropriate alternative natural substance carrying an antibacterial capacity would be immensely beneficial. It has been previously discovered that the different types of cupric salts, especially copper sulfate pentahydrate (CuSO4·5H2O), to carry a potent bactericidal capacity. We investigated the neutralizing effect of CuSO4·5H2O (6.25μg/ml) on the reactive oxygen species generation, and expression of MyD88, an essential adaptor protein of Toll-like receptor, and NF-κB in three intestinal epithelial cell lines exposed to 50ng/ml lipopolysaccharide. In order to find the optimal cupric sulfate concentration without enteritis-inducing toxicity, broiler chickens were initially fed with water containing 0.4, 0.5, and 1mg/l during a period of 4days. After determination of appropriate dosage, two broiler chickens and turkey flocks with enteritis were fed with cupric compound for 4days. We found that cupric sulfate can lessen the cytotoxic effect of lipopolysaccharide by reducing the reactive oxygen species content (p<0.05). Additionally, the expression of MyD88 and NF-κB was remarkably down-regulated in the presence of lipopolysaccharide and cupric sulfate. The copper sulfate in doses lower than 0.4mg/ml expressed no cytotoxic effect on the liver, kidney, and the intestinal tract while a concentration of 0.5 and 1mg/ml contributed to a moderate to severe tissue injuries. Pearson Chi-Square analysis revealed the copper cation significantly diminished the rate of mortality during 4-day feeding of broiler chicken and turkey with enteritis (p=0.000). Thus, the results briefed above all confirm the potent anti-bactericidal feature of cupric sulfate during the course of enteritis.

View Article
May 2017
3 Reads

Investigation of gene expression and serum levels of PIN1 and eNOS with high blood pressure in patients with Alzheimer disease.

J Clin Neurosci. 2017 May 12

J Clin Neurosci

According to evidence, Alzheimer's disease is known as one of the most serious neurodegenerative diseases, for which hypertension has been observed to be a key risk factor. Therefore, this study aims to examine the relationship between the PIN1 and eNOS genes expression, as well as serum levels and hypertension in Alzheimer's disease sufferers. Blood samples were obtained from subjects who were divided into four groups: the control group, normotensive Alzheimer's patients, the Alzheimer's sufferers group with hypertension, and the healthy group with only hypertension, considering the inhibition of confounding factors. Thereafter, eNOS and PIN1 genes expression along with serum levels were studied. Based on the obtained results, a statistically significant correlation didn't exist between serum level of PIN1 and the systolic and diastolic blood pressure, between serum level of eNOS and diastolic blood pressure in the norm tension Alzheimer's disease patients, between serum levels of PIN1, eNOS and systolic blood pressure, and between serum eNOS and systolic and diastolic blood pressure in the patients with hypertension (p<0.05). According to the results obtained from this study, measuring the serum levels of eNOS and Pin1 may contribute to the prognosis, prevention, and monitoring of hypertension and also to the reduction of death rates from cardiovascular diseases in Alzheimer's disease.

View Article
May 2017
5 Reads

Exosomes and their Application in Biomedical Field: Difficulties and Advantages.

Mol Neurobiol. 2017 May 11

Mol Neurobiol

The science of gene therapy has experienced a controversial history. At first, the initial concept that various disorders become curable by gene transferring was very exciting and challengeable. However, the problems and difficulties related to emerging techniques and unwanted side effects seen in some patients who have undergone gene therapy make some questions against the safety of novel molecular medicine approach. In line with this statement, discovery and developing a good bio-vector possessing low toxicity and high efficiency rate are the most important issues in gene therapy field. Introducing exosomes as vectors for gene delivery gives us a new opportunity in gene-based therapy. Exosomes, ranging from 30 to 120 nm in diameter, have unique lipid and protein composition. These nanostructures participate in cell-to-cell cross-talk, regulation of immune system, and the transport of genetic material. Besides the inherent potency of exosomes in gene therapy, a better understanding of their biology, characteristics, production, targeting, and cargo loading still need to be elucidated. In the current review, we exclusively focused on the various facets of exosomes and their importance as a bio-shuttle in gene therapy.

View Article
May 2017
5 Reads

Protective effects of melatonin on long-term administration of fluoxetine in rats.

Exp Toxicol Pathol

The degree and consequence of tissue injury are highly regarded during long-term exposure to selective antidepressant fluoxetine. Melatonin has been shown to palliate different lesions by scavenging free radicals, but its role in the reduction of the fluoxetine-induced injuries has been little known. Thirty-six mature male Wistar rats were randomly assigned into control and experimental groups. The experimental rats were included as following; 24mg/kg/bw fluoxetine for 4 weeks; 1mg/kg/bw melatonin for 4 weeks; fluoxetine+1-week melatonin, fluoxetine+2-week melatonin and fluoxetine+4-week melatonin. In the current experiment, we investigated weight gain, hematological and biochemical parameters, pathological injuries and oxidative status. We noted the positive effect of melatonin in weight loss of fluoxetine-treated rats (p<0.05). The significant reduction of superoxide dismutase, glutathione peroxidase, catalase activities in blood, liver, and kidneys and changes in serum total antioxidant capacity caused by fluoxetine were reversed by melatonin (p<0.05). Melatonin reduced the increased lipid peroxidation and transaminase activity in rats received fluoxetine (p<0.05). We also showed the potency of fluoxetine in inducing leukopenia, thrombocytopenia and hypochromic and macrocytic anemia which was blunted by melatonin. Both RBCs and platelets indices were also corrected. Rats received melatonin in combination with fluoxetine showed a reduction in the severity of degeneration and inflammatory changes in different tissues, brain, heart, liver, lungs, testes and kidneys as compared to the fluoxetine group. Therefore, melatonin fundamentally reversed the side effects of fluoxetine in the rat model which is comparable to human medicine

View Article
May 2017
5 Reads

Role of glycogen synthase kinase following myocardial infarction and ischemia-reperfusion.

Apoptosis. 2017 Apr 18

Apoptosis

Glycogen synthase kinase-3 beta (GSK3β) is principally is a glycogen synthase phosphorylating enzyme that is well known for its role in muscle metabolism. GSK3β is a serine/threonine protein Kinase, which is responsible for several essential roles in mammalian cells. This enzyme is implicated in the pathophysiology of many conditions involved in homeostasis and cellular immigration. GSK3β is involved in several pathways leading to neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Increasing evidence has shown the potential importance of GSK3β in ischemic heart disease and ischemia-reperfusion pathologies. Reperfusion injury may occur in tissues after prolonged ischemia following reperfusion. Reperfusion injury can be life threatening. Reperfusion injury occurs due to a change in ionic homeostasis, excess free radical production, mitochondrial damage and cell death. There are however clear, cardiac-protective signals; although the molecular pathophysiology is not clearly understood. In normal physiology, GSK3β has a critical role in the cytoprotective pathway. However, it`s controversial role in ischemia and ischemia-reperfusion is a topic of current interest. In this review, we have opted to focus on GSK3β interactions with mitochondria in ischemic heart disease and expand on the therapeutic interventions.

View Article
April 2017
4 Reads

Bone marrow mesenchymal stem cells and their conditioned media could potentially ameliorate ovalbumin-induced asthmatic changes.

Biomed Pharmacother 2017 Jan 5;85:28-40. Epub 2016 Dec 5.

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2016.11.127DOI Listing
January 2017
13 Reads
3 Citations
2.023 Impact Factor

The role of morphine on rat neural stem cells viability, neuro-angiogenesis and neuro-steroidgenesis properties.

Neurosci Lett 2017 01 11;636:205-212. Epub 2016 Nov 11.

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S03043940163086
Publisher Site
http://dx.doi.org/10.1016/j.neulet.2016.11.025DOI Listing
January 2017
20 Reads
2.060 Impact Factor

Contributory Anti-Inflammatory Effects of Mesenchymal Stem Cells, Not Conditioned Media, On Ovalbumin-Induced Asthmatic Changes in Male Rats.

Inflammation 2016 Dec;39(6):1960-1971

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s10753-016-0431-2
Publisher Site
http://dx.doi.org/10.1007/s10753-016-0431-2DOI Listing
December 2016
9 Reads
1 Citation
1.921 Impact Factor

The roles of non-coding RNAs in Parkinson's disease.

Mol Biol Rep 2016 Nov 5;43(11):1193-1204. Epub 2016 Aug 5.

Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11033-016-4054-3DOI Listing
November 2016
34 Reads
11 Citations
2.024 Impact Factor

Effects of combination of melatonin and laser irradiation on ovarian cancer cells and endothelial lineage viability.

Lasers Med Sci 2016 Nov 30;31(8):1565-1572. Epub 2016 Jun 30.

Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10103-016-2016-6DOI Listing
November 2016
12 Reads
3 Citations
2.490 Impact Factor

Stem Cells as a Promising Tool for the Restoration of Brain Neurovascular Unit and Angiogenic Orientation.

Mol Neurobiol.

Establishing systemic therapeutics for neuro-inflammatory diseases is a main objective of regenerative medicine, certainly focusing on restoration of BBB dysfunction and neurovascular reconstitution. In this context, the regenerative capacity and therapeutic effects of different stem cells have been recently found to bring hopeful outcomes through trans-differentiation, paracrine interactions, and molecular cross-talk of transplanted stem cells with resident cells of blood-brain barrier. Here, we highlight some of the most recent scientific breakthroughs and discoveries by different authorities. Potency of different types of stem cells on the regulation and maintenance of endothelial and perivascular cells via various microRNAs was also studied.

View Article
November 2016
52 Reads

A reversal of age-dependent proliferative capacity of endothelial progenitor cells from different species origin in in vitro condition.

J Cardiovasc Thorac Res 2016 26;8(3):102-106. Epub 2016 Sep 26.

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.15171/jcvtr.2016.22DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5075357PMC
September 2016
5 Reads
1 Citation

A reversal of age-dependent proliferative capacity of endothelial progenitor cells from different species origin in in vitro condition

J Cardiovasc Thorac Res. 2016; 8(3): 102–106.

Journal of Thoracic and Cardiovascular Research

Introduction: A large number of cardiovascular disorders and abnormalities, notably accelerated vascular deficiencies could be related to aging changes and increased length of life. During the past decades, the discovery of different stem cells facilitates ongoing attempts for attenuating many disorders, especially in vascular beds. Endothelial progenitor cells (EPCs) are a subtype of stem cells that have potent capacity to differentiate into mature endothelial cells (ECs). However, some documented studies reported an age-related decline in proliferation and function of many stem cells. There is no data on aging effect upon proliferation and morphological feature of EPCs. Methods: To show aging effect on EPCs proliferation and multipotentiality, bone marrow samples were provided from old and young cases in three different species; human, mouse and dog. After 7 days of culture, the cell morphology and clonogenic capacity were evaluated. We also calculated the mean number of colonies both in bone marrow samples from old and young subjects. To confirm the cell phenotype, isolated cells were immune-phenotyped by a panel of antibodies against Tie-2, CD133 and CD309 markers. Results: Our results showed that EPCs exhibited prominent spindle form in all bone marrow samples from young cases while the cell shape became more round by aging. Notably, the number of colonies was reduced in aged samples as compared to parallel young subject samples (P < 0.05). We also detected that the expression of endothelial related markers diminished by aging. Conclusion: The results of this study suggest that the age-related vascular abnormalities could be presumably related to the decline in stemness capacity of EPCs.

View Article
September 2016
4 Reads

ECM-Dependence of Endothelial Progenitor Cell Features.

J Cell Biochem 2016 08 2;117(8):1934-46. Epub 2016 Feb 2.

Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcb.25492DOI Listing
August 2016
10 Reads
3 Citations
3.263 Impact Factor

Morphine Inhibited the Rat Neural Stem Cell Proliferation Rate by Increasing Neuro Steroid Genesis.

Neurochem Res 2016 Jun 30;41(6):1410-9. Epub 2016 Jan 30.

Department of Medical Education, Medical Education Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s11064-016-1847-7
Publisher Site
http://dx.doi.org/10.1007/s11064-016-1847-7DOI Listing
June 2016
6 Reads
2.593 Impact Factor

Potent anti-angiogenic and cytotoxic effect of conferone on human colorectal adenocarcinoma HT-29 cells.

Phytomedicine 2016 Apr 21;23(4):398-405. Epub 2016 Feb 21.

Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran; Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.phymed.2016.01.015DOI Listing
April 2016
19 Reads
2 Citations
3.130 Impact Factor

Endothelial juxtaposition of distinct adult stem cells activates angiogenesis signaling molecules in endothelial cells.

Cell Tissue Res 2015 Dec 12;362(3):597-609. Epub 2015 Jun 12.

Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00441-015-2228-2DOI Listing
December 2015
13 Reads
2 Citations
3.565 Impact Factor

Evaluation of Motor Neuron-Like Cell Differentiation of hEnSCs on Biodegradable PLGA Nanofiber Scaffolds.

Mol Neurobiol 2015 Dec 7;52(3):1704-1713. Epub 2014 Nov 7.

Brain and Spinal Cord Injury Research Center (BASIR), Tehran University of Medical Sciences, Keshavarz Boulevard, Gharib Street, 6114185, Tehran, Iran.

View Article

Download full-text PDF

Source
http://link.springer.com/content/pdf/10.1007/s12035-014-8931
Web Search
http://link.springer.com/10.1007/s12035-014-8931-2
Publisher Site
http://dx.doi.org/10.1007/s12035-014-8931-2DOI Listing
December 2015
22 Reads
2 Citations
5.140 Impact Factor

Paracrine Neuroprotective Effects of Neural Stem Cells on Glutamate-Induced Cortical Neuronal Cell Excitotoxicity.

Adv Pharm Bull 2015 Nov 30;5(4):515-21. Epub 2015 Nov 30.

Neural Stem Cell and Regenerative Neuroscience Laboratory, Department of Anatomical Sciences, Shiraz School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.; Neural Stem Cell and Regenerative Neuroscience Laboratory, Shiraz Stem Cell Institute, Shiraz University of Medical Sciences, Shiraz, Iran.

View Article

Download full-text PDF

Source
http://journals.tbzmed.ac.ir/APB/Abstract/APB_2651_201501072
Publisher Site
http://dx.doi.org/10.15171/apb.2015.070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729347PMC
November 2015
10 Reads
1 Citation

Evaluation of Motor Neuron-Like Cell Differentiation of hEnSCs on Biodegradable PLGA Nanofiber Scaffolds.

Mol Neurobiol. 2014 Nov 7. [Epub ahead of print]

Molecular Neurobiology

Human endometrium is a high-dynamic tissue that contains human endometrial stem cells (hEnSCs) which can be differentiated into a number of cell lineages. The differentiation of hEnSCs into many cell lineages such as osteoblast, adipocyte, and neural cells has been investigated previously. However, the differentiation of these stem cells into motor neuron-like cells has not been investigated yet. Different biochemical and topographical cues can affect the differentiation of stem cells into a specific cell. The aim of this study was to investigate the capability of hEnSCs to be differentiated into motor neuron-like cells under biochemical and topographical cues. The biocompatible and biodegradable poly(lactic-co-glycolic acid) (PLGA) electrospun nanofibrous scaffold was used as a topographical cue. Human EnSCs were cultured on the PLGA scaffold and tissue culture polystyrene (TCP), then differentiation of hEnSCs into motor neuron-like cells under induction media including retinoic acid (RA) and sonic hedgehog (Shh) were evaluated for 15 days. The proliferation rate of cells was assayed by using MTT assay. The morphology of cells was studied by scanning electron microscopy imaging, and the expression of motor neuron-specific markers by real-time PCR and immunocytochemistry. Results showed that survival and differentiation of hEnSCs into motor neuron-like cells on the PLGA scaffold were better than those on the TCP group. Taken together, the results suggest that differentiated hEnSCs on PLGA can provide a suitable, three-dimensional situation for neuronal survival and outgrowth for regeneration of the central nervous system, and these cells may be a potential candidate in cellular therapy for motor neuron diseases

View Article
November 2014
4 Reads

Effect of dexamethasone, insulin and EGF on the myogenic potential on human endometrial stem cell.

Iran J Pharm Res 2014 ;13(2):659-64

Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

View Article

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157042PMC
September 2014
46 Reads

Dynamic induction of pro-angiogenic milieu after transplantation of marrow-derived mesenchymal stem cells in experimental myocardial infarction.

Int J Cardiol 2014 May 15;173(3):453-66. Epub 2014 Mar 15.

Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology (TAIWAN TECH), Taipei, 10607, Taiwan.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijcard.2014.03.008DOI Listing
May 2014
22 Reads
8 Citations
4.040 Impact Factor

Flow cytometric immunophenotyping of feline bone marrow cells and haematopoietic progenitor cells using anti-human antibodies.

J Feline Med Surg 2014 Apr 24;16(4):265-74. Epub 2013 Sep 24.

1Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1177/1098612X13505575DOI Listing
April 2014
21 Reads
1.160 Impact Factor

Serological proteome analysis of dogs with breast cancer unveils common serum biomarkers with human counterparts.

Electrophoresis 2014 Mar 28;35(6):901-10. Epub 2014 Jan 28.

Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran; Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/elps.201300461DOI Listing
March 2014
10 Reads
2 Citations
3.030 Impact Factor

Interactions of mesenchymal stem cells with endothelial cells.

Stem Cells Dev 2014 Feb 28;23(4):319-32. Epub 2013 Nov 28.

Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran , Tehran, Iran .

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1089/scd.2013.0419DOI Listing
February 2014
5 Reads
19 Citations
3.730 Impact Factor

Isolation and characterization of a canine mammary cell line prepared for proteomics analysis.

Tissue Cell 2013 Jun 29;45(3):183-90. Epub 2013 Jan 29.

Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tice.2012.11.002DOI Listing
June 2013
5 Reads
1 Citation
1.050 Impact Factor

Haemoglobin typing and its variations in Iranian domestic dogs.

Comp Clin Path 2012 Dec 2;21(6):1515-1519. Epub 2011 Sep 2.

College of Veterinary Medicine, University of Tehran, Tehran, Iran.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00580-011-1322-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509321PMC
December 2012
3 Reads

Top co-authors

Alireza Nourazarian
Alireza Nourazarian

Tabriz University of Medical Sciences

12
Seyed Mahdi Nassiri
Seyed Mahdi Nassiri

University of Tehran

10
Aysa Rezabakhsh
Aysa Rezabakhsh

Urmia University

9
Mohammad Nouri
Mohammad Nouri

Tabriz University of Medical Sciences

9
Majid Khaksar
Majid Khaksar

Stem Cell Research Center

8
Mahdi Ahmadi
Mahdi Ahmadi

Tabriz University of Medical Sciences

7
Mehdi Hassanpour
Mehdi Hassanpour

University of Birjand

6
Jafar Rezaie
Jafar Rezaie

Solid Tumor Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences

6
Omid Cheraghi
Omid Cheraghi

University of Tabriz

6
Rana Keyhanmanesh
Rana Keyhanmanesh

Tabriz University of Medical Sciences

6

Followers