UiTM Shah Alam
Shah Alam, Selangor | Malaysia
Main Specialties: Infectious Disease
My name is Fakharul. I was originally born in Malaysia and now I’m a senior lecturer at Universiti Teknologi MARA, Shah Alam, Malaysia. My research interest in biofilms began when a friend of mine asked me how to study biocorrosion. Currently I am studying the potential application of natural products to control a wide range of biofilm-forming bacteria
Primary Affiliation: UiTM Shah Alam - Shah Alam, Selangor , Malaysia
M.F.Z.R. Yahya, M.S.A. Ibrahim, W.M.A.W.M. Zawawi and U.M.A. Hamid, 2014. Biofilm Killing Effects of Chromolaena odorata Extracts against Pseudomonas aeruginosa. Research Journal of Phytochemistry, 8: 64-73.
Research Journal of Phytochemistry
Chromolaena odorata is known to possess antimicrobial effects against a wide range of microorganisms including Pseudomonas aeruginosa. However, the inhibitory effects of Chromolaena odorata extracts against the biofilm growth mode of Pseudomonas aeruginosa remain uncertain. Therefore, this study was carried out to determine the antibiofilm activity of Chromolaena odorata extracts against Pseudomonas aeruginosa under aerobic and anaerobic conditions. Phytochemical screening using gas chromatography mass spectrometry revealed the major constituent in both Chromolaena odorata chloroform and ethanolic extracts, namely germacrene D. All microbial tests were carried out under aerobic and anaerobic conditions. Based on microbroth dilution assay performed, oxygen level did not show any effect towards the minimum inhibitory concentration and minimum bactericidal concentration of Chromolaena odorata extracts against Pseudomonas aeruginosa. However, antibacterial susceptibility test showed that the size of inhibition zones of Chromolaena odorata extracts against Pseudomonas aeruginosa were slightly different between the aerobic and anaerobic conditions. Colony forming unit counting of biofilm cells demonstrated that Chromolaena odorata chloroform extract had greater antibiofilm activity against Pseudomonas aeruginosa as compared to Chromolaena odorata ethanolic extract under aerobic condition. In contrast,Chromolaena odorata ethanolic extract showed greater antibiofilm activity against Pseudomonas aeruginosa biofilm than Chromolaena odorata chloroform extract in the absence of oxygen. Furthermore, treatment of both Chromolaena odorata chloroform and ethanolic extracts resulted in changes in biochemical composition of Pseudomonas aeruginosa biofilm extracellular matrixes under both experimental conditions, as indicated by variation in the infrared spectra in the region between 1700 and 900 cm-1. We conclude that the antibiofilm activities of Chromolaena odorata extracts depend on solvent extraction method and oxygen level. The findings from this study would improve the existing antimicrobial treatment plan to combat facultative anaerobic Pseudomonas aeruginosa biofilm.
M.F.Z.R. Yahya, U.M.A. Hamid, M.Y. Norfatimah and R. Kambol, 2014. In silico Analysis of Essential Tricarboxylic Acid Cycle Enzymes from Biofilm-forming Bacteria. Trends in Bioinformatics, 7: 19-26.
Trends in Bioinformatics
The Tricarboxylic Acid Cycle (TCA) cycle is the central point in the metabolism of living organisms and is important for the survival of infectious biofilms. The inhibition of this vital point could be a promising strategy for the control of infectious biofilms. Therefore, this study was carried out to identify the potential drug targets from the TCA cycle of several Biofilm-Forming Bacteria (BFB) and to identify the available small molecule drugs against the TCA cycle enzymes. Based on the in silico substractive genomic approach, citrate lyase subunit alpha/citrate CoA-transferase [EC: 184.108.40.206], succinate dehydrogenase iron-sulfur subunit (EC: 220.127.116.11) and 2-oxoglutarate ferredoxin oxidoreductase subunit delta [EC: 18.104.22.168] were found to be essential and exclusively present in the BFB. Further in silico analyses showed that most of them are chemically regulated by myristoylation, phosphorylation, glycosylation and amidation. Based on the sequence search against DrugBank database, the potential small molecule drugs for biofilm treatment are 2-[1-methylhexyl]-4, 6-dinitrophenol, Atpenin A5 and Ubiquinone-2 which all target the succinate dehydrogenase enzyme of BFB. This study demonstrates the rapid identification of potential drug targets and small molecule drugs which could be useful in biofilm control strategies.
Folia Microbiol (Praha) 2018 Jan 24;63(1):23-30. Epub 2017 May 24.
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
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