Dr. Mohd Fakharul Zaman Raja Yahya, PhD - UiTM Shah Alam - Dr.

Dr. Mohd Fakharul Zaman Raja Yahya

PhD

UiTM Shah Alam

Dr.

Shah Alam, Selangor | Malaysia

Main Specialties: Infectious Disease

Dr. Mohd Fakharul Zaman Raja Yahya, PhD - UiTM Shah Alam - Dr.

Dr. Mohd Fakharul Zaman Raja Yahya

PhD

Introduction

My name is Fakharul. I was originally born in Malaysia and now I’m a senior lecturer at Universiti Teknologi MARA, Shah Alam, Malaysia. My research interest in biofilms began when a friend of mine asked me how to study biocorrosion. Currently I am studying the potential application of natural products to control a wide range of biofilm-forming bacteria

Primary Affiliation: UiTM Shah Alam - Shah Alam, Selangor , Malaysia

Specialties:

Research Interests:

Education

Oct 2018
University of Malaya, Kuala Lumpur
PhD
Biofilmology

Experience

Sep 2019
Differential whole-cell proteome expression and protein interaction network in the biofilm of Corynebacterium pseudotuberculosis clinical isolates
Principal Investigator
National Grant
Sep 2014
A study on inhibition of Streptococcus mutans growth and disruption of established biofilm on orthodontic appliances using biosurfactant
Co-Investigator
National Grant
Sep 2012
Integral polytopic proteome of surface-attached Pseudomonas aeruginosa biofilm
Principal Investigator
National Grant

Publications

4Publications

160Reads

45Profile Views

Biofilm Killing Effects of Chromolaena odorata Extracts against Pseudomonas aeruginosa

Authors:
MFZR Yahya

M.F.Z.R. Yahya, M.S.A. Ibrahim, W.M.A.W.M. Zawawi and U.M.A. Hamid, 2014. Biofilm Killing Effects of Chromolaena odorata Extracts against Pseudomonas aeruginosa. Research Journal of Phytochemistry, 8: 64-73.

Research Journal of Phytochemistry

Chromolaena odorata is known to possess antimicrobial effects against a wide range of microorganisms including Pseudomonas aeruginosa. However, the inhibitory effects of Chromolaena odorata extracts against the biofilm growth mode of Pseudomonas aeruginosa remain uncertain. Therefore, this study was carried out to determine the antibiofilm activity of Chromolaena odorata extracts against Pseudomonas aeruginosa under aerobic and anaerobic conditions. Phytochemical screening using gas chromatography mass spectrometry revealed the major constituent in both Chromolaena odorata chloroform and ethanolic extracts, namely germacrene D. All microbial tests were carried out under aerobic and anaerobic conditions. Based on microbroth dilution assay performed, oxygen level did not show any effect towards the minimum inhibitory concentration and minimum bactericidal concentration of Chromolaena odorata extracts against Pseudomonas aeruginosa. However, antibacterial susceptibility test showed that the size of inhibition zones of Chromolaena odorata extracts against Pseudomonas aeruginosa were slightly different between the aerobic and anaerobic conditions. Colony forming unit counting of biofilm cells demonstrated that Chromolaena odorata chloroform extract had greater antibiofilm activity against Pseudomonas aeruginosa as compared to Chromolaena odorata ethanolic extract under aerobic condition. In contrast,Chromolaena odorata ethanolic extract showed greater antibiofilm activity against Pseudomonas aeruginosa biofilm than Chromolaena odorata chloroform extract in the absence of oxygen. Furthermore, treatment of both Chromolaena odorata chloroform and ethanolic extracts resulted in changes in biochemical composition of Pseudomonas aeruginosa biofilm extracellular matrixes under both experimental conditions, as indicated by variation in the infrared spectra in the region between 1700 and 900 cm-1. We conclude that the antibiofilm activities of Chromolaena odorata extracts depend on solvent extraction method and oxygen level. The findings from this study would improve the existing antimicrobial treatment plan to combat facultative anaerobic Pseudomonas aeruginosa biofilm.

View Article
June 2019
29 Reads

In silico Analysis of Essential Tricarboxylic Acid Cycle Enzymes from Biofilm-forming Bacteria

Authors:
MFZR Yahya

M.F.Z.R. Yahya, U.M.A. Hamid, M.Y. Norfatimah and R. Kambol, 2014. In silico Analysis of Essential Tricarboxylic Acid Cycle Enzymes from Biofilm-forming Bacteria. Trends in Bioinformatics, 7: 19-26.

Trends in Bioinformatics

The Tricarboxylic Acid Cycle (TCA) cycle is the central point in the metabolism of living organisms and is important for the survival of infectious biofilms. The inhibition of this vital point could be a promising strategy for the control of infectious biofilms. Therefore, this study was carried out to identify the potential drug targets from the TCA cycle of several Biofilm-Forming Bacteria (BFB) and to identify the available small molecule drugs against the TCA cycle enzymes. Based on the in silico substractive genomic approach, citrate lyase subunit alpha/citrate CoA-transferase [EC: 4.1.3.6], succinate dehydrogenase iron-sulfur subunit (EC: 1.3.99.1) and 2-oxoglutarate ferredoxin oxidoreductase subunit delta [EC: 1.2.7.3] were found to be essential and exclusively present in the BFB. Further in silico analyses showed that most of them are chemically regulated by myristoylation, phosphorylation, glycosylation and amidation. Based on the sequence search against DrugBank database, the potential small molecule drugs for biofilm treatment are 2-[1-methylhexyl]-4, 6-dinitrophenol, Atpenin A5 and Ubiquinone-2 which all target the succinate dehydrogenase enzyme of BFB. This study demonstrates the rapid identification of potential drug targets and small molecule drugs which could be useful in biofilm control strategies.

View Article
June 2019
32 Reads

Antibiofilm activity and mode of action of DMSO alone and its combination with afatinib against Gram-negative pathogens.

Folia Microbiol (Praha) 2018 Jan 24;63(1):23-30. Epub 2017 May 24.

Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12223-017-0532-9DOI Listing
January 2018
85 Reads
1.145 Impact Factor

Subtractive Protein Profiling of Salmonella typhimurium Biofilm Treated with DMSO.

Protein J 2017 08;36(4):286-298

Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10930-017-9719-9DOI Listing
August 2017
75 Reads
0.912 Impact Factor