Dr. Mohamad Hafizi Abu Bakar, PhD - Universiti Sains Malaysia - Senior Lecturer

Dr. Mohamad Hafizi Abu Bakar

PhD

Universiti Sains Malaysia

Senior Lecturer

Minden, Pulau Pinang | Malaysia

Main Specialties: Biology, Biotechnology, Endocrinology Diabetes & Metabolism

Additional Specialties: Cell-based assays (in vitro); metabolomics

ORCID logohttps://orcid.org/0000-0001-8064-695X

Dr. Mohamad Hafizi Abu Bakar, PhD - Universiti Sains Malaysia - Senior Lecturer

Dr. Mohamad Hafizi Abu Bakar

PhD

Introduction

Dr. Mohamad Hafizi Abu Bakar is a Senior Lecturer at the Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia (USM). He obtained the First Class Honor in Bachelor of Biomedical Science (Hons) from International Islamic University Malaysia (IIUM) in July 2012. Subsequently, he successfully completed his PhD study in Bioprocess Engineering at the Universiti Teknologi Malaysia (UTM) in December 2015 under supervision of Prof. Dr. Mohamad Roji Sarmidi. During UTM 56th Convocation Ceremony, he received the 'Best Postgraduate Student Award' (PhD category) in Bioprocess Engineering.

Primary Affiliation: Universiti Sains Malaysia - Minden, Pulau Pinang , Malaysia

Specialties:

Additional Specialties:

Research Interests:

Education

Sep 2012 - Dec 2015
Universiti Teknologi Malaysia
Doctor of Philosophy (Bioprocess Engineering)
Bioprocess Engineering
Jul 2008 - Jun 2012
International Islamic University Malaysia
Bachelor of Biomedical Sciences (Honours)
Biomedical Sciences

Experience

Jul 2016 - Jul 2016
Universiti Sains Malaysia
Senior Lecturer
Bioprocess Technology Division, School of Industrial Technology
Feb 2015 - May 2015
Universiti Teknologi Malaysia
Academic Fellow
Bioprocess Engineering

Publications

14Publications

385Reads

25Profile Views

31PubMed Central Citations

Banana frond juice as novel fermentation substrate for bioethanol production by Saccharomyces cerevisiae

2019 Aug 19; 21:101293

Biocatalysis and Agricultural Biotechnology

Bioethanol has been produced from sugars originating from starchy staple crops such as wheat, sugarcane or corn. However, these sugar- and starch-containing feedstocks are predominantly used for food and feed, which affecting their continuous supply. In Malaysia, banana residual is the second largest agricultural waste after oil palm wastes. Banana fronds need only a milling process for the extraction of sugars to fermentation medium, and ethanol can be produced directly from juice. The sugars and minerals composition of the banana frond juice (BFJ) and the effects of BFJ concentration, addition of nitrogen sources and pH were studied. The pressed juice of the banana fronds was found to contain a total sugar of 14% with the amount of glucose (18.9 g/L), sucrose (13.29 g/L) and fructose (15.63 g/L) with total volume of 0.33 L of BFJ/kg of banana frond. The BFJ at 80% (v/v) supplemented with yeast extract at 15 g/L with optimum pH at 6.8 successfully increased the bioethanol concentration to 42.47 g/L. The optimized fermentation conditions in shake flask were scaled up to 2 L bioreactor. The bioethanol concentration that obtained in the bioreactor system was 45.75 g/L, which is 0.33 g ethanol/g sugar or 65% of the theoretical yield. The results obtained from this study showed the potential of production of sugars from BFJ for the subsequent production of bioethanol and further optimization on the parameters of bioreactor is needed to improve the bioethanol production.

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August 2019

Withaferin A Protects Against High-Fat Diet-Induced Obesity Via Attenuation of Oxidative Stress, Inflammation, and Insulin Resistance.

Appl Biochem Biotechnol 2019 May 12;188(1):241-259. Epub 2018 Nov 12.

Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800, Gelugor, Penang, Malaysia.

Withaferin A (WA), a bioactive constituent derived from Withania somnifera plant, has been shown to exhibit many qualifying properties in attenuating several metabolic diseases. The current investigation sought to elucidate the protective mechanisms of WA (1.25 mg/kg/day) on pre-existing obese mice mediated by high-fat diet (HFD) for 12 weeks. Following dietary administration of WA, significant metabolic improvements in hepatic insulin sensitivity, adipocytokines with enhanced glucose tolerance were observed. The hepatic oxidative functions of obese mice treated with WA were improved via augmented antioxidant enzyme activities. The levels of serum pro-inflammatory cytokines and hepatic mRNA expressions of toll-like receptor (TLR4), nuclear factor ?B (NF-?B), tumor necrosis factor-? (TNF-?), chemokine (C-C motif) ligand-receptor, and cyclooxygenase 2 (COX2) in HFD-induced obese mice were reduced. Mechanistically, WA increased hepatic mRNA expression of peroxisome proliferator-activated receptors (PPARs), cluster of differentiation 36 (CD36), fatty acid synthase (FAS), carnitine palmitoyltransferase 1 (CPT1), glucokinase (GCK), phosphofructokinase (PFK), and phosphoenolpyruvate carboxykinase (PCK1) that were associated with enhanced lipid and glucose metabolism. Taken together, these results indicate that WA exhibits protective effects against HFD-induced obesity through attenuation of hepatic inflammation, oxidative stress, and insulin resistance in mice.

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http://dx.doi.org/10.1007/s12010-018-2920-2DOI Listing
May 2019
25 Reads
2.140 Impact Factor

Synthesis, characterization and cytotoxicity of new nicotinonitriles and their furo[2,3-b]pyridine derivatives

2019 April; 16(4): 715-722

Journal of the Iranian Chemical Society

The present research work describes the synthesis of new series of nicotinonitrile (2) and furo[2,3-b]pyridine (3) heterocycles bearing thiophene substituent. The nicotinonitrile derivatives were prepared from the corresponding 3-cyano-(2H)-pyridones (1a–f) in excellent yields. The ring cyclization of the nicotinonitrile derivatives (2a–f) in the presence of sodium methoxide provided the furo[2,3-b]pyridines (3a–f) in moderate to good yields. All the newly synthesized compounds were characterized by extensive NMR analysis data, including 1D-NMR experiments (1H and 13C) and 2D-NMR experiments (COSY, HMBC, HSQC), as well as HRESIMS data. All the final products were subjected for cytotoxic activity against five different tumour cell lines including HeLa (cervical), DU145 (prostate), HepG2 (liver), MDA-MB-231 (breast-ER negative) and MCF7 (breast-ER positive), in addition to HSF1184 (normal human fibroblast) using the MTT assay. Compounds 2d and 3e were found to exhibit promising cytotoxicity with IC50 of < 20 µM against all different tested tumour cell lines. In addition, these compounds showed high selectivity (40–287 folds) for tumour cells over normal cells.

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April 2019

Impact Factor 1.742

Reduced mitochondrial DNA content in lymphocytes is associated with insulin resistance and inflammation in patients with impaired fasting glucose.

Clin Exp Med 2018 Aug 17;18(3):373-382. Epub 2018 Mar 17.

Clinical Investigation Centre, University Malaya Medical Centre, 59100, Lembah Pantai, Kuala Lumpur, Malaysia.

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http://dx.doi.org/10.1007/s10238-018-0495-4DOI Listing
August 2018
51 Reads
2.824 Impact Factor

Extractive purification of recombinant thermostable lipase from fermentation broth of using an aqueous polyethylene glycol impregnated resin system.

3 Biotech 2018 Jun 9;8(6):288. Epub 2018 Jun 9.

6School of Engineering, Taylor's University, No. 1 Jalan Taylor's, 47500 Subang Jaya, Selangor Malaysia.

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http://dx.doi.org/10.1007/s13205-018-1295-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994206PMC
June 2018
8 Reads

Molecular docking studies of bioactive compounds from Annona muricata Linn as potential inhibitors for Bcl-2, Bcl-w and Mcl-1 antiapoptotic proteins.

Apoptosis 2018 01;23(1):27-40

Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia.

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http://dx.doi.org/10.1007/s10495-017-1434-7DOI Listing
January 2018
17 Reads
3.685 Impact Factor

Improvement of mitochondrial function by celastrol in palmitate-treated C2C12 myotubes via activation of PI3K-Akt signaling pathway.

Biomed Pharmacother 2017 Sep 14;93:903-912. Epub 2017 Jul 14.

Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800 Gelugor, Penang, Malaysia.

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https://linkinghub.elsevier.com/retrieve/pii/S07533322173201
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http://dx.doi.org/10.1016/j.biopha.2017.07.021DOI Listing
September 2017
15 Reads
1 Citation
2.023 Impact Factor

Association of cultured myotubes and fasting plasma metabolite profiles with mitochondrial dysfunction in type 2 diabetes subjects.

Mol Biosyst 2017 Aug;13(9):1838-1853

Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800 Gelugor, Penang, Malaysia.

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http://dx.doi.org/10.1039/c7mb00333aDOI Listing
August 2017
9 Reads

Purification of β-mannanase derived from Bacillus subtilis ATCC 11774 using ionic liquid as adjuvant in aqueous two-phase system.

J Chromatogr B Analyt Technol Biomed Life Sci 2017 Jun 17;1055-1056:104-112. Epub 2017 Apr 17.

Bioprocess Technology, School of Industrial Technology, Universiti Sains Malaysia, 11800 Penang, Malaysia. Electronic address:

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http://dx.doi.org/10.1016/j.jchromb.2017.04.029DOI Listing
June 2017
40 Reads
1 Citation
2.730 Impact Factor

Celastrol attenuates mitochondrial dysfunction and inflammation in palmitate-mediated insulin resistance in C3A hepatocytes.

Eur J Pharmacol 2017 Mar 1;799:73-83. Epub 2017 Feb 1.

Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia.

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http://dx.doi.org/10.1016/j.ejphar.2017.01.043DOI Listing
March 2017
31 Reads
2 Citations
2.532 Impact Factor

Metabolomics - the complementary field in systems biology: a review on obesity and type 2 diabetes.

Mol Biosyst 2015 Jul;11(7):1742-74

Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia.

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http://dx.doi.org/10.1039/c5mb00158gDOI Listing
July 2015
56 Reads
13 Citations

Mitochondrial dysfunction as a central event for mechanisms underlying insulin resistance: the roles of long chain fatty acids.

Diabetes Metab Res Rev 2015 Jul 18;31(5):453-75. Epub 2014 Oct 18.

Department of Pharmacy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

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http://dx.doi.org/10.1002/dmrr.2601DOI Listing
July 2015
117 Reads
10 Citations

Celastrol Protects against Antimycin A-Induced Insulin Resistance in Human Skeletal Muscle Cells.

Molecules 2015 May 7;20(5):8242-69. Epub 2015 May 7.

Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

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http://dx.doi.org/10.3390/molecules20058242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272652PMC
May 2015
5 Reads
2 Citations
2.420 Impact Factor

Amelioration of mitochondrial dysfunction-induced insulin resistance in differentiated 3T3-L1 adipocytes via inhibition of NF-κB pathways.

Int J Mol Sci 2014 Dec 2;15(12):22227-57. Epub 2014 Dec 2.

Innovation Centre in Agritechnology for Advanced Bioprocessing (ICA), University Teknologi Malaysia, Skudai 81310, Malaysia.

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http://dx.doi.org/10.3390/ijms151222227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284705PMC
December 2014
11 Reads
2 Citations
2.862 Impact Factor

Top co-authors

Mohamad Roji Sarmidi
Mohamad Roji Sarmidi

Universiti Teknologi Malaysia

6
Joo Shun Tan
Joo Shun Tan

Universiti Putra Malaysia

5
Hasniza Zaman Huri
Hasniza Zaman Huri

University of Malaya

5
Harisun Yaakob
Harisun Yaakob

Universiti Teknologi Malaysia

4
Sahar Abbasiliasi
Sahar Abbasiliasi

Universiti Putra Malaysia

2
Kian-Kai Cheng
Kian-Kai Cheng

University of Cambridge

2
Cheng Kian Kai
Cheng Kian Kai

University Teknologi Malaysia

2
Hui Suan Ng
Hui Suan Ng

Universiti Putra Malaysia

2
Yew Joon Tam
Yew Joon Tam

Universiti Putra Malaysia

1