Dasharath Bhanudasrao Shinde, PhD, Pursuing - Symbiosis School of Biological Sciences (SSBS) Symbiosis International (Deemed University)  - Ph.D. Student

Dasharath Bhanudasrao Shinde

PhD, Pursuing

Symbiosis School of Biological Sciences (SSBS) Symbiosis International (Deemed University)

Ph.D. Student

Pune, Maharashtra | India

Additional Specialties: Lactic acid bacteria, Probiotics, Probiotics food applications, Whole Genome sequencing and comparative genomics.

Dasharath Bhanudasrao Shinde, PhD, Pursuing - Symbiosis School of Biological Sciences (SSBS) Symbiosis International (Deemed University)  - Ph.D. Student

Dasharath Bhanudasrao Shinde

PhD, Pursuing

Introduction

Primary Affiliation: Symbiosis School of Biological Sciences (SSBS) Symbiosis International (Deemed University) - Pune, Maharashtra , India

Additional Specialties:

Research Interests:

Education

Sep 2018
Symbiosis Center for Research and Innovation (SCRI), Pune
Ph.D in Biotechnology
Pursuing
Jun 2015
Symbiosis School of Biomedical Sciences, Pune
Masters in Biotechnology
Symbiosis International University (SIU), Pune
Jun 2015
Symbiosis School of Biomedical Sciences, Pune
Masters in Biotechnology
Symbiosis International University (SIU), Pune
Jun 2013
MGM's Institute of Bioscience and Technology, Aurangabad
Bachelor's in Biotechnology
Dr. BAMU Aurangabad
Jun 2013
MGM's Institute of Bioscience and Technology, Aurangabad
Bachelor's in Biotechnology
Dr. BAMU Aurangabad
Jun 2013
MGM's Institute of Bioscience and Technology, Aurangabad
Bachelor's in Biotechnology
Dr. BAMU Aurangabad

Experience

Jul 2015
Research Assistant
In vitro and In vivo studies
Work till 31 july 2017

Publications

1Publications

-Reads

4Profile Views

5PubMed Central Citations

ANTIOXIDANT ACTIVITY AND ANTIPROLIFERATIVE ACTION OF METHANOLIC EXTRACT OF LIQUORICE (GLYCYRRHIZA GLABRA) IN HEPG2 CELL LINE

Shinde, D. B., S. S. Koratkar, N. Sharma, and A. A. Shitole. “ANTIOXIDANT ACTIVITY AND ANTIPROLIFERATIVE ACTION OF METHANOLIC EXTRACT OF LIQUORICE (GLYCYRRHIZA GLABRA) IN HEPG2 CELL LINE”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 9, Sept. 2016, pp. 293-8, doi:10.221

International Journal of Pharmacy and Pharmaceutical Sciences

ABSTRACT

Objective: To evaluate the in vitro antioxidant activity of liquorice (Glycyrrhiza glabra) against H2O2 induced oxidative stress in HepG2 cell line.

Methods: Antioxidant activity of methanolic extracts of Glycyrrhiza glabra was investigated by measuring total phenolic content using folin-ciocalteu reagent (FCR), free radical scavenging activity by DPPH and ferric reducing antioxidant power (FRAP). The presence of phenolic compounds and flavonoids in the extract was confirmed by Liquid Chromatography-Mass Spectrometry (LC-MS) analysis. Furthermore, the protective effect of methanolic extract of Glycyrrhiza glabra against oxidative stress induced by H2Oin HepG2 cells was investigated by MTT assay. HepG2 cells were exposed with five different treatments viz. liquorice, H2O2, ascorbic acid, H2O2+liquorice and H2O2+ascorbic acid, to explore the effect of the extract on malondialdehyde (MDA) production, catalase activity, and glutathione reductase levels.

Results: The total phenolic content estimated in Glycyrrhiza glabra extract was found to be 241.47 µg per 1000 µg/ml of methanolic extract. It was found that as the concentration of the extract was increased both the free radical scavenging activity and ferric ion reducing power was also found to increase. LC-MS analysis confirmed the presence of eight different phenolic compounds in the methanolic extract which are possibly contributing to the antioxidant activity exhibited by the extract. It was also observed that liquorice treated HepG2 cells showed lower MDA and higher glutathione and catalase levels as compared to only H2Otreated HepG2 cells where increased MDA production, decreased glutathione reductase and catalase production was observed.

Conclusion: Our results thus conclude that, the methanolic extract of Glycyrrhiza glabra can be used as natural supplements in various disease conditions where oxidative stress has been reported.

KEYWORDS: Liquorice, Oxidative stress, H2O2, HepG2 cells, Antioxidant activity, Free radicals, MDA, SOD, Catalase

View Article
September 2016

5 Citations

54 Reads