Publications by authors named "Zuowei Li"

34 Publications

Pancancer Analysis of Neurovascular-Related NRP Family Genes as Potential Prognostic Biomarkers of Bladder Urothelial Carcinoma.

Biomed Res Int 2021 15;2021:5546612. Epub 2021 Apr 15.

Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Background: Neurovascular-related genes have been implicated in the development of cancer. Studies have shown that a high expression of neuropilins (NRPs) promotes tumourigenesis and tumour malignancy.

Method: A multidimensional bioinformatics analysis was performed to examine the relationship between NRP genes and prognostic and pathological features, tumour mutational burden (TMB), microsatellite instability (MSI), and immunological features based on public databases and find the potential prognostic value of NRPs in pancancer.

Results: Survival analysis revealed that a low NRP1 expression in adrenocortical carcinoma (ACC), cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC), low-grade glioma (LGG), and stomach adenocarcinoma (STAD) was associated with poor prognosis. A high NRP2 expression in bladder urothelial carcinoma (BLCA), kidney renal papillary cell carcinoma (KIRP), and mesothelioma (MESO) was associated with poor prognosis. Moreover, NRP1 and NRP2 were associated with TMB and MSI. Subsequent analyses showed that NRP1 and NRP2 were correlated with immune infiltration and immune checkpoints. Genome-wide association analysis revealed that the NRP1 expression was strongly associated with kidney renal clear cell carcinoma (KIRC), whereas the NRP2 expression was closely associated with BLCA. Ultimately, NRP2 was found to be involved in the development of BLCA.

Conclusions: Neurovascular-related NRP family genes are significantly correlated with cancer prognosis, TME, and immune infiltration, particularly in BLCA.
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http://dx.doi.org/10.1155/2021/5546612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062179PMC
June 2021

FAM46B Promotes Apoptosis and Inhibits Glycolysis of Prostate Cancer Through Inhibition of the MYC-LDHA Axis.

Onco Targets Ther 2020 1;13:8771-8782. Epub 2020 Sep 1.

Department of Urology, Shanghai Sixth People's Hospital East Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai 201306, People's Republic of China.

Objective: Increased dependence on glycolysis is a known element of cancer. This study was designed to examine critical glycolysis components including transcription factor MYC and its downstream target lactate dehydrogenase A (LDHA), potential upstream regulators of glycolysis such as family with sequence similarity 46 member B (FAM46B), and the impact of the abundance of these proteins on apoptosis and glycolysis in prostate cancer.

Materials And Methods: A total of 70 primary prostate cancer patient samples were compared to normal tissues for FAM46B and LDHA expression and the corresponding patients' survival was monitored for 60 months. Prostate cancer cell lines were employed for protein expression manipulation, glucose uptake and LDH assays, and apoptosis measurements. A xenograft mouse model was used to quantify the role of FAM46B and LDHA on tumor growth in vivo.

Results: FAM46B expression was reduced in prostate tumor tissue compared to normal tissue and prostate cancer patients who expressed low amounts of FAM46B had shortened average lifespans compared to those who expressed higher amounts of FAM46B (=0.008). FAM46B overexpression reduced glucose uptake, decreased LDH activity, and induced apoptosis in prostate cancer cell lines while FAM46B shRNA increased MYC levels in a non-malignant prostate cell line (P69). Conversely, forced expression of LDHA in LNCaP cells produced an increase in glycolysis markers with a corresponding decrease in apoptosis. FAM46B-overexpressing xenografts had starkly blunted growth which was restored with co-overexpression of LDHA.

Conclusion: FAM46B plays a central role in regulating glycolysis and apoptosis in prostate cancer and operates through the regulation of LDHA via MYC. FAM46B's keystone status in prostate cancer makes it a potential, robust biomarker for prostate cancer prognosis and a promising therapeutic target.
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http://dx.doi.org/10.2147/OTT.S258724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7478375PMC
September 2020

Structural elucidation of the degradation mechanism of nickel-rich layered cathodes during high-voltage cycling.

Chem Commun (Camb) 2020 May 1;56(36):4886-4889. Epub 2020 Apr 1.

School of Chemical Engineering, Sichuan University, Chengdu 610065, P. R. China.

Phase transition occurring during cycling plays a fundamentally important role in the cycling performance of nickel-rich cathodes. Here, splitting of two O3 phases, rather than the often observed O1 phases in the conventional LiCoO electrode, was discovered in LiNiCoMnO at a high-voltage region (>4.6 V). Such degradation could be mitigated via Al doping.
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http://dx.doi.org/10.1039/d0cc00327aDOI Listing
May 2020

Biosynthesis of selenium nanoparticles and their effect on changes in urinary nanocrystallites in calcium oxalate stone formation.

3 Biotech 2020 Jan 20;10(1):23. Epub 2019 Dec 20.

1Department of Urology, Shanghai Sixth People's Hospital East Affiliated to Shanghai University of Medicine and Health Sciences, Shanghai, 201306 People's Republic of China.

Plant bio constituents have the ability to prepare nanoparticles, and usually, plant polyphenols are tested to reduce sodium selenite to selenium nanoparticles (SeNPs). In this work, we showed the biosynthesis of SeNPs using leaf extract. The as obtained SeNPs were in the size range of 15-20 nm and spherical in shape. Also, TEM microscopic images represented the aggregation of crystal structures as extracellular deposits. Moreover, scanning electron microscopy was performed to examine the chemical transition of calcium oxalate (CaCO) crystal's shape and structure due to the influence of SeNPs. SeNPs inhibited the aggregation and growth of CaCO monohydrate crystals and hence the prepared SeNPs could have important prospects in medical and pharmaceutical applications as a potential inhibitor of CaCO urinary stones.
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http://dx.doi.org/10.1007/s13205-019-1999-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925084PMC
January 2020

MSRB3 promotes the progression of clear cell renal cell carcinoma via regulating endoplasmic reticulum stress.

Pathol Res Pract 2020 Feb 2;216(2):152780. Epub 2019 Dec 2.

Department of Urology, Shanghai Sixth People's Hospital East Affiliated to Shanghai University of Medicine & Health Science, Shanghai, 201306, China. Electronic address:

Background: Renal cancer represents about 3 % of all human cancers. Clear cell renal cell carcinoma (ccRCC) is the main type of renal cancer. Methionine sulfoxide reductase B3 (MSRB3) is a protein repair enzyme that specifically catalyzes the reduction of methionine-R-sulfoxide residues and has an antioxidant function. However, MSRB3's role in ccRCC is still obscure.

Methods: Immunohistochemical staining and Real-time PCR were used to compare the expression level of MSRB3 in ccRCC tissues and adjacent tissues. Western blot was used to detect the expression of MSRB3 in cell lines. Chi-square test were applied to evaluate the potential of MSRB3 to function as a cancer biomarker. RNA interference was used to inhibit MSRB3 expression in ccRCC cells, followed by detecting cell proliferation, apoptosis, migration and invasion. The markers of endoplasmic reticulum stress were then detected by western blot.

Results: In this study, we validated that MSRB3 was significantly up-regulated in ccRCC samples and cell lines. It was also demonstrated that the up-regulation of MSRB3 was associated with several clinicopathologic features. Knockdown of MSRB3 remarkably arrested the proliferation, migration and invasion, while promoted apoptosis, and induced the changes of markers of endoplasmic reticulum stress.

Conclusion: In conclusion, we demonstrated that MSRB3 was an oncogene of ccRCC associated with patients' pathological characteristics and modulated endoplasmic reticulum stress of cancer cells.
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http://dx.doi.org/10.1016/j.prp.2019.152780DOI Listing
February 2020

Long noncoding RNA TM1P3 is involved in osteoarthritis by mediating chondrocyte extracellular matrix degradation.

J Cell Biochem 2019 08 19;120(8):12702-12712. Epub 2019 Mar 19.

Department of Surgery, School of Medicine, Hunan Normal University, Changsha, Hunan, China.

Background And Objectives: Osteoarthritis (OA) is a widespread degenerative joint disease characterized by articular cartilage degradation and is the leading cause of physical disability. Noncoding RNAs, especially long noncoding RNAs (lncRNAs) and microRNAs, are involved in the degradation of the chondrocyte extracellular matrix (ECM) in patients with OA. The present study was aimed to investigate the effects of lncRNA and miR-22 on the degradation of the chondrocyte ECM and underlying mechanisms.

Methods: To simulate conditions found in OA, primary cultured chondrocytes were treated with IL-1, TGF-β, or sb525334. Real-time PCR and Western blot analysis were performed to detect expressions of miR-22, lncRNA-TM1P3, ALK1, MMP13, pSMAD1/5, SMAD1, and pSMAD5. Small interfering RNAs and a miR-22 mimic or inhibitor were utilized to determine lncRNA-TM1P3 knockdown and miR-22 overexpression or inhibition.

Results: The lncRNA-TM1P3 significantly upregulated in patients with OA, accompanied by the downregulation of miR-22 and upregulation of pSMAD1/5 and MMP13, which ultimately resulted in the degradation of the chondrocyte ECM in patients with OA. Bioinformatics analysis predicted miR-22 as a target of both lncRNA-TM1P3 and MMP13. The lncRNA-TM1P3 knockdown significantly increased the expression of ALK1, a corresponding increase in ECM degradation was observed by affecting the phosphorylation of SMAD1/5 and the expression of MMP13, which did not affect the expression of ALK1.

Conclusions: These findings demonstrated that the lncRNA-TM1P3/miR-22/TGF-β signaling/MMP13 axis is involved in the degradation of chondrocyte ECM in patients with OA, which could provide novel therapies for OA treatment.
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http://dx.doi.org/10.1002/jcb.28539DOI Listing
August 2019

Down-Regulation of Nfatc1 Suppresses Proliferation, Migration, Invasion, and Warburg Effect in Prostate Cancer Cells.

Med Sci Monit 2019 Feb 28;25:1572-1581. Epub 2019 Feb 28.

Department of Urology, Shanghai Sixth People's Hospital East Affiliated to Shanghai University of Medicine and Health Sciences, Shanghai, China (mainland).

BACKGROUND Prostate cancer (PCa), accounting for 28% of all male cancer cases, is the second leading cause of cancer-related death among men. NFATc1, belonging to the NFAT family, is overexpressed in PCa and is correlated with the risk of recurrence after radical prostatectomy. MATERIAL AND METHODS In the present study, the expression of NFATc, c-myc, and PKM2 in PCa cells was regulated by lentiviruses and then detected by real-time PCR and Western blot analysis. Further, proliferation, invasion, and migration assays were performed. The glucose consumption and lactate production were assessed by biochemical detection. RESULTS We found that NFATc1 down-regulation significantly suppressed the proliferation and Warburg effect of PCa cells, concurrent with a decrease of c-myc and PKM2 expression. Likewise, the abilities of migration and invasion were also inhibited in NFATc1-silenced PCa cells. In addition, NFATc1 down-regulation-induced inhibition of cell proliferation, migration, invasion, and Warburg effect were counteracted by up-regulation of c-myc or PKM2. The expression of PKM2 was positively regulated by NFATc1 and c-myc expression. CONCLUSIONS These results indicate that NFATc1 down-regulation can suppress the proliferation, Warburg effect, and migration and invasion abilities of PCa cells, probably by regulating c-myc and PKM2 expression. NFATc1 may be a potential therapeutic target for PCa and could be used as a diagnosis or prognosis indicator of PCa.
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http://dx.doi.org/10.12659/MSM.910998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404685PMC
February 2019

FAM46B inhibits cell proliferation and cell cycle progression in prostate cancer through ubiquitination of β-catenin.

Exp Mol Med 2018 12 10;50(12):1-12. Epub 2018 Dec 10.

Department of Urology, Shanghai Sixth People's Hospital East Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai, 201306, China.

FAM46B is a member of the family with sequence similarity 46. Little is known about the expression and functional role(s) of FAM46B in prostate cancer (PC). In this study, the expression of FAM46B expression in The Cancer Genome Atlas, GSE55945, and an independent hospital database was measured by bioinformatics and real-time PCR analysis. After PC cells were transfected with siRNA or a recombinant vector in the absence or presence of a β-catenin signaling inhibitor (XAV-939), the expression levels of FAM46B, C-myc, Cyclin D1, and β-catenin were measured by western blot and real-time PCR. Cell cycle progression and cell proliferation were measured by flow cytometry and the CCK-8 assay. The effects of FAM46B on tumor growth and protein expression in nude mice with PC tumor xenografts were also measured. Our results showed that FAM46B was downregulated but that β-catenin was upregulated in patients with PC. FAM46B silencing promoted cell proliferation and cell cycle progression in PC, which were abrogated by XAV-939. Moreover, FAM46B overexpression inhibited PC cell cycle progression and cell proliferation in vitro and tumor growth in vivo. FAM46B silencing promoted β-catenin protein expression through the inhibition of β-catenin ubiquitination. Our data clearly show that FAM46B inhibits cell proliferation and cell cycle progression in PC through ubiquitination of β-catenin.
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http://dx.doi.org/10.1038/s12276-018-0184-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288130PMC
December 2018

Repair of urethral defects by an adipose mesenchymal stem cell‑porous silk fibroin material.

Mol Med Rep 2018 Jul 9;18(1):209-215. Epub 2018 May 9.

Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.

The aim of the present study was to determine whether it was possible to repair urethral defects with a material of adipose mesenchymal stem cells (ADMSCs)‑porous silk fibroin (SF). A total of 39 male New Zealand white rabbits were randomly divided into a control group, an SF group and a bromodeoxyuridine (BrdU)‑labeled ADMSCs‑SF group (SSF group; n=13/group). Defects were made by resecting the posterior urethral wall. The defects in the SF and SSF groups were repaired using SF and BrdU‑labeled ADMSCs‑SF materials respectively. Then the anterior wall was sutured, and the urethral catheter was retained for 3 weeks following surgery. The catheter was rinsed with nitrofurazone once a day. The cells with positive expressions of factor VIII related antigen (FVIII‑RAg), α‑smooth muscle actin (α‑SMA) and pan‑cytokeratin (AE1/AE3) were detected by immunohistochemical assay, and the distributions of BrdU positive cells and macrophages were observed. Urethrography was performed prior to and following surgery. All rabbits had normal urethral morphologies prior to surgery. The incidence rates of postoperative complications in the control, SF and SSF groups were 76.92 (7/13), 23.07 (3/13) and 15.38% (2/13), respectively (P<0.05). The number of positive macrophages in the SSF group was significantly lower than that of the SF group 4 weeks following surgery (P<0.05). In the SSF group, BrdU positive cells were scattered within the SF material following surgery, primarily at the intersection between the SF material and the urethra. The number of FVIII‑RAg positive cells in the SSF and SF groups were significantly different (P<0.05), which were also significantly higher than that of control group (P<0.01). The number of α‑SMA positive cells in the SSF and SF groups were significantly different (P<0.05), and these values also significantly exceeded those exhibited by the control group (P<0.01). In addition, the SSF and SF groups had positive staining of AE1/AE3. Similar to normal urethral mucosa, the cytoplasm was stained brownish yellow (P<0.05). It is thus feasible to repair urethral defects using ADMSCs‑SF material.
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http://dx.doi.org/10.3892/mmr.2018.9001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059671PMC
July 2018

Dynamic high pressure induced strong and weak hydrogen bonds enhanced by pre-resonance stimulated Raman scattering in liquid water.

Opt Express 2017 Dec;25(25):31670-31677

355 nm pulsed laser is employed to excite pre-resonance forward stimulated Raman scattering (FSRS) of liquid water at ambient temperature. Due to the shockwave induced dynamic high pressure, the obtained Raman spectra begin to exhibit double peaks distribution at 3318 and 3373 cm with the input energy of 17 mJ,which correspond with OH stretching vibration with strong and weak hydrogen (H) bonds. With laser energy rising from 17 to 27 mJ, the Stokes line at 3318 cm shifts to 3255 and 3230 cm because of the high pressure being enlarged. When the energy is up to 32 mJ, only 3373 cm peak exists. The strong and weak H bond exhibit quite different energy dependent behaviors.
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http://dx.doi.org/10.1364/OE.25.031670DOI Listing
December 2017

Concentration Effect on Quenching of Chlorophyll a Fluorescence by All-Trans-β-Carotene in Photosynthesis.

Molecules 2017 Sep 21;22(10). Epub 2017 Sep 21.

Coherent Light and Atomic and Molecular Spectroscopy Laboratory, College of Physics, Jilin University, Changchun 130012, China.

Absorption, fluorescence spectra of chlorophyll a (Chl-a) and all-trans-β-carotene (β-Car) mixing solution are investigated in different polarity and polarizability solvents. The carotenoids regulate the energy flow in photosynthesis by interaction with chlorophyll, leading to an observable reduction of Chl-a fluorescence. The fluorescence red shifts with the increasing solvent polarizability. The energy transfer in the Chl-a and β-Car system is proposed. The electron transfer should be dominant in quenching Chl-a fluorescence rather than the energy transfer in this system. Polar solvent with large polarizability shows high quenching efficiency. When dissolved in carbon tetrachloride, Chl-a presents red shift of absorption and blue shift of fluorescence spectra with increasing β-Car concentration, which implies a Chl-a conformational change.
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http://dx.doi.org/10.3390/molecules22101585DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151392PMC
September 2017

Effects of Acupuncture on mRNA Levels of Apoptotic Factors in Perihematomal Brain Tissue During the Acute Phase of Cerebral Hemorrhage.

Med Sci Monit 2017 Mar 30;23:1522-1532. Epub 2017 Mar 30.

General Hospital of Loandjili, Pointe-Noire, Congo, the Democratic Republic of the.

BACKGROUND To explore the time-dependent effects of acupuncture on mRNA levels of the apoptotic factors BCL-2 and BAX in a rat cerebral hemorrhage model, slow injection of autologous blood to the caudate nucleus was used to generate the cerebral hemorrhage model. MATERIAL AND METHODS A sham surgery control group, groups with acupuncture applied 3, 9, 24, and 48 hours after model induction, and time-matched model-only control groups were used. In situ hybridization was used to detect BCL-2 and BAX mRNA expression, and semi-quantitative RT-PCR was used to measure the expression. RESULTS The number of BCL-2 and BAX mRNA-positive cells significantly increased during the acute phase of cerebral hemorrhage. BCL-2 mRNA was significantly upregulated in acupuncture groups compared to other groups, whereas BAX mRNA levels in the acupuncture groups were lower in the other groups, except for the sham surgery group. Additionally, earlier acupuncture intervention was associated with a lower ratio of expression between the two genes. Changes in BCL-2 and BAX mRNA expression were consistent with changes in the number of cells positive for BCL-2 and BAX mRNA; however, the change in the expression ratio was consistent with the change in the number of cells positive for BCL-2 mRNA, but opposite to the change in the number of cells positive for BAX mRNA. CONCLUSIONS Acupuncture ameliorated changes in expression of apoptotic factors in the brain induced by acute cerebral hemorrhage and may thus protect the brain, with greater efficacy when the delay before acupuncture was minimized.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384619PMC
http://dx.doi.org/10.12659/msm.897689DOI Listing
March 2017

Curcumin inhibits urothelial tumor development by suppressing IGF2 and IGF2-mediated PI3K/AKT/mTOR signaling pathway.

J Drug Target 2017 Aug 26;25(7):626-636. Epub 2017 Mar 26.

a Department of Urology , Shanghai Jiao Tong University Affiliated Sixth People's Hospital , Shanghai , China.

We have previously reported that curcumin inhibits urothelial tumor development in a rat bladder carcinogenesis model. In this study, we report that curcumin inhibits urothelial tumor development by suppressing IGF2 and IGF2-mediated PI3K/AKT/mTOR signaling pathway. Curcumin inhibits IGF2 expression at the transcriptional level and decreases the phosphorylation levels of IGF1R and IRS-1 in bladder cancer cells and N-methyl-N-nitrosourea (MNU)-induced urothelial tumor tissue. Ectopic expression of IGF2 and IGF1R, but not IGF1, in bladder cancer cells restored this process, suggesting that IGF2 is a target of curcumin. Moreover, introduction of constitutively active AKT1 abolished the inhibitory effect of curcumin on cell proliferation, migration, and restored the phosphorylation levels of 4E-BP1 and S6K1, suggesting that curcumin functions via suppressing IGF2-mediated AKT/mTOR signaling pathway. In summary, our results reveal that suppressing IGF2 and IGF2-mediated PI3K/AKT/mTOR signaling pathway is one of the mechanisms of action of curcumin. Our findings suggest a new therapeutic strategy against human bladder cancer caused by aberrant activation of IGF2, which are useful for translational application of curcumin.
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http://dx.doi.org/10.1080/1061186X.2017.1306535DOI Listing
August 2017

RNF7 knockdown inhibits prostate cancer tumorigenesis by inactivation of ERK1/2 pathway.

Sci Rep 2017 03 2;7:43683. Epub 2017 Mar 2.

Shanghai Sixth People's Hospital East, Shanghai University of Medicine &Health Sciences, Shanghai 201306, China.

Development of castration resistance is a key contributor to mortality in patients with prostate cancer. High expression of RING finger protein 7 (RNF7) in cancer cells is known to play a key role in tumor progression. However, the role of RNF7 in prostate cancer progression is not well elucidated. In this study, we silenced RNF7 by shRNA interference in two castration resistant prostate cancer (CRPC) cell lines, DU145 and PC3. RNF7 knockdown attenuated proliferation and enhanced sensitivity of prostate cancer cells to cisplatin treatment. Invasive property of DU145 and PC3 cells was also attenuated by RNF7 silencing. The underlying mechanisms appear to be associated with accumulation of tumor suppressive proteins p21, p27 and NOXA, while inactivation of ERK1/2 by RNF7 knockdown. We demonstrated that RNF7 knockdown induced growth suppression of prostate cancer cells and inactivated ERK1/2 pathway, which suggested RNF7 might be a potential novel therapeutic target for CRPC.
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http://dx.doi.org/10.1038/srep43683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333079PMC
March 2017

The Chlorophyll a Fluorescence Modulated by All-Trans-β-Carotene in the Process of Photosystem II.

Int J Mol Sci 2016 Jun 21;17(6). Epub 2016 Jun 21.

Coherent Light and Atomic and Molecular Spectroscopy Laboratory, College of Physics, Jilin University, Changchun 130012, China.

Modulating the chlorophyll a (Chl-a) fluorescence by all-trans-β-Carotene (β-Car) in the polarity and non-polarity solutions was investigated. The fluorescence intensity of Chl-a decreased as the concentration of β-Car increased. The excited electronic levels of Chl-a and β-Car became much closer owing to the solvent effect, which led to the electron transfer between both two molecules. A electron-separated pair Chl(-)·Chl⁺ that is not luminous was formed due to electron transfer. The solution of Chl-a and β-car in C₃H₆O was similar to the internal environment of chloroplast. We conclude that the polar solvent is good for the fluorescent modulation in photosystem II.
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http://dx.doi.org/10.3390/ijms17060978DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926510PMC
June 2016

Controlling cross pumping between C-N and C-H vibration in nitromethane by selective fluorescence-enhanced stimulated Raman scattering.

Opt Express 2016 May;24(9):10132-41

To investigate the vibrational features of nitromethane (NM), which is a kind of energy material and a well known low-sensitivity and high explosive, experiments are performed to obtain the stimulated Raman scattering (SRS) of NM by employing a 532 nm pulsed pump laser. The Raman signal involves two stimulated emissions at 918 and 2,963 cm-1, attributed to the C-N and C-H stretching vibrations, respectively. To overcome the complexity of cross pump in the pure NM, one stimulated Raman radiation is chosen as a pump source to excite the other Raman mode. Two fluorescence dyes were added to selectively enhance each Raman cross section. By internally seeding the Raman gain medium with fluorescent photons, a significant modification in the stimulated Raman scattering spectrum has been observed. The enhanced Stokes emission at 918 cm-1 was able to induce the 2,963 cm-1 vibration mode when the all-trans-β-carotene was internal seeding in the NM, while the Raman radiation at 2,963 cm-1 was enhanced to excite the C-N mode with the addition of m-Cresol purple. The output energy of both 918 and 2,963 cm-1 under different input energy was also measured to illustrate this result.
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http://dx.doi.org/10.1364/OE.24.010132DOI Listing
May 2016

Pre-resonance-stimulated Raman scattering for water bilayer structure on laser-induced plasma bubble surface.

Opt Lett 2015 Jul;40(14):3253-5

Pre-resonance-stimulated Raman scattering (PSRS) from water molecules in the air/water interfacial regions was studied when the laser-induced plasma bubble was generated at the interfaces. A characteristically lower Raman shift of OH-stretching vibrational modes of water molecules at around 3000  cm(-1) (370 meV) was observed, in which the mechanisms were possibly attributed to the strong hydrogen bond in a well-ordered water bilayer structure that was formed on a laser-induced plasma bubble surface. Simultaneously, the PSRS of ice Ih at about 3100  cm(-1) was obtained, which also belonged to the strong hydrogen bond effect in ice Ih structure.
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http://dx.doi.org/10.1364/OL.40.003253DOI Listing
July 2015

Hydrated-electron resonance enhancement O-H stretching vibration of water hexamer at air-water interface.

Opt Lett 2015 Apr;40(7):1434-7

Raman scattering of the O-H stretching vibration mode inside water, as well as near and at the air-water interface, was investigated by laser-induced breakdown (LIB). An intense and characteristic higher wavenumber Raman shift of the O-H vibration was observed at the air-water interface, which was attributed to the hydrated-electron resonance enhancement of the O-H stretching vibration mode of water hexamer. The hydrated electron in the water hexamer structure was formed by excess electrons injected into the gas-like phase with low hydrogen bond order under LIB. The electron-phonon coupled mechanism was discussed.
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http://dx.doi.org/10.1364/OL.40.001434DOI Listing
April 2015

A study on the Fermi resonance of phenol under the effects of pressure and temperature by Raman spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc 2015 Feb 30;136 Pt C:1767-74. Epub 2014 Oct 30.

College of Physics, Jilin Normal University, Siping 136000, PR China. Electronic address:

The ν1-ν18a Fermi resonance (FR) of phenol were investigated by pressure-dependent Raman spectroscopy from atmospheric up to P=15.2GPa and temperature-dependent Raman spectroscopy from 40 down to T=-180°C, respectively. In the case of pressure, we found the Fermi coupling coefficient W, which were calculated based on the FR theory, exposed a value turnover between 1.912 and 2.244GPa in the process of increasing the pressure. This turnover phenomenon of the Fermi coupling coefficient W has been ascribed to the crystal structure of phenol evolving towards a more symmetric structure with pressure, from a structure like a pseudo-threefold helical chain at ambient pressure to like a ribbon arrangement at 1.912GPa, then to adopt a herringbone arrangement at much higher pressure. On the other hand, we also found the Fermi coupling coefficient W exhibited monotonic reduction without turnover points appearing by decreasing the temperature. The tendencies of the Fermi coupling coefficient W with temperature were in good agreement with the pressure dependence of the Fermi coupling coefficient W in the region of ambient to 1.912GPa, indicating that the effect of pressure and temperature on the FR of phenol in this region might be the same. A conformation evolving induced by pressure and temperature on the ν1 and ν18a FR of phenol have been analyzed.
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http://dx.doi.org/10.1016/j.saa.2014.10.080DOI Listing
February 2015

Temperature induced changes in resonance Raman spectra intensity of all-trans-β-carotene: changes in the fundamental, combination and overtone modes.

Spectrochim Acta A Mol Biomol Spectrosc 2014 Oct 24;131:153-7. Epub 2014 Apr 24.

College of Physics, Jilin University, Changchun 130012, PR China. Electronic address:

The resonance Raman spectra of the fundamental, combination and overtone modes around the CC and CC stretches of all-trans-β-carotene in 1,2-dichloroethane solution are obtained from the 293K to 83K temperature range. The results indicate that the intensity of the fundamentals in the liquid and solid phases generally increases as the temperature decreases, except for the liquid-solid phase transition, which exhibits a decreasing trend. The Raman intensities ratio between the fundamentals υ1 and υ2, combinations (overtones) and the fundamentals both increases with decreasing temperature. The Raman bandwidths of the CC bonds gradually become narrow as the temperature decreases. These varieties of relative intensity are analyzed using the coherent weakly damped electron-lattice vibration mode, the effective conjugation length mode as well as the theory of electron-phonon interaction in this work.
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http://dx.doi.org/10.1016/j.saa.2014.04.069DOI Listing
October 2014

Anharmonic coupling between fundamental modes in tetramethylurea.

J Chem Phys 2014 Apr;140(16):164310

State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012, People's Republic of China.

In situ high pressure Raman spectra of tetramethylurea have been measured up to 25 GPa, liquid-solid and solid-solid phase transitions were detected at 0.2 GPa and 7.4 GPa, respectively. An unprecedented spectral phenomenon is the observation of a Fermi resonance between the fundamental modes. An exponential relationship between the intensity and the frequency difference was concluded. Pressure provides us a new way to study the correlation between Fermi resonance parameters.
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http://dx.doi.org/10.1063/1.4872159DOI Listing
April 2014

Phase-dominant pressure-induced planar molecular conformation of S-trioxane.

J Phys Chem B 2013 Jul 15;117(29):8911-7. Epub 2013 Jul 15.

State Key Laboratory of Superhard Materials, Jilin University, Changchun, PR China 130012.

In situ high-pressure Raman spectra of S-trioxane have been measured up to 28 GPa. A first-order phase transition was detected at ~3 GPa from the splitting, newly existing and diminishing of the internal modes and from changes in the slope on plots of frequency versus pressure. The vibrational spectra and theoretical simulation indicate that the isolated molecule structure changes from puckered to very puckered structure at the first phase, while at the beginning of the second phase, S-trioxane goes back to its original puckered structure and loses its C3 axis; then, it changes to planar structure at about ~GPa and keeps its flat structure in the second phase up to the highest pressure studied. We believe that phase may be a dominant factor responsible for the pressure-induced planar molecular geometry, providing a reasonable explanation for the experimental observations.
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http://dx.doi.org/10.1021/jp404320tDOI Listing
July 2013

Stimulated Raman scattering from sulfur-II produced by laser decomposition of liquid carbon disulfide.

Opt Lett 2013 Mar;38(6):950-2

State Key Laboratory of Superhard Materials, College of Physics, Jilin University, Changchun, China.

Stimulated Raman scattering (SRS) of sulfur-II (S-II) phase was investigated by laser decomposition of liquid carbon disulfide. As a matter of fact, above a threshold of the laser intensity, it is suggested that a strong shock wave is generated in the liquid carbon disulfide, which is decomposed owing to the induced high dynamic pressure and temperature. One bending mode E frequency at 289 cm(-1) and one symmetric stretching mode A1 frequency at 490 cm(-1) of S-II phase were observed. The SRS spectra indicated that S-II structure is formed by laser decomposition, as the strong shock wave generates the stable pressure-temperature range of S-II phase. The dynamic high-pressure and static-electric field generated by laser-induced breakdown results in the softening A1 mode becoming more hardened.
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http://dx.doi.org/10.1364/OL.38.000950DOI Listing
March 2013

Upregulated H19 contributes to bladder cancer cell proliferation by regulating ID2 expression.

FEBS J 2013 Apr 6;280(7):1709-16. Epub 2013 Mar 6.

Department of Urology, Affiliated Tenth People's Hospital, Tongji University, Shanghai, China.

Long noncoding RNAs have been shown to have important regulatory roles in cancer biology, and long noncoding RNA 19 (H19) is essential for human tumor growth. However, little is known about how abnormal expression of H19 contributes to bladder cancer cell proliferation. In this study, we first evaluated the expression of H19 in bladder cancer tissues by real-time PCR, and defined the biological functions. We found that H19 expression levels were remarkably increased in bladder cancer tissues as compared with adjacent normal control tissue, and forced expression of H19 promoted bladder cancer cell proliferation in vitro. Inhibitor of DNA binding/differentiation 2 (ID2) expression levels were upregulated in bladder cancer tissues and in bladder cancer cells. A significant positive correlation was observed between H19 levels and ID2 levels in vivo. We further demonstrated that overexpression of H19 resulted in a significant increase in the expression of ID2, whereas H19 knockdown decreased ID2 expression in vitro. Gain-of-function and loss-of-function studies demonstrated that upregulated H19 increased bladder cancer cell proliferation by increasing ID2 expression. In conclusion, upregulated H19 increases bladder cancer growth by regulating ID2 expression, and thus may be helpful in the development of effective treatment strategies for bladder cancer.
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http://dx.doi.org/10.1111/febs.12185DOI Listing
April 2013

Long non-coding RNA H19 increases bladder cancer metastasis by associating with EZH2 and inhibiting E-cadherin expression.

Cancer Lett 2013 Jun 24;333(2):213-21. Epub 2013 Jan 24.

Department of Urology, The Tenth People's Hospital Affiliated to Tongji University, Shanghai 200072, China.

lncRNA H19 is essential for human tumor growth. However, little is known about whether H19 regulates bladder cancer metastasis. Here we found that H19 levels are remarkably increased in bladder cancer tissues, and upregulated H19 promotes bladder cancer cell migration in vitro and in vivo. H19 is associated with enhancer of zeste homolog 2 (EZH2), and that this association results in Wnt/β-catenin activation and subsequent downregulation of E-cadherin. A significant negative correlation is also observed between H19 levels and E-cad levels in vivo. These data suggest that upregulated H19 enhances bladder cancer metastasis by associating with EZH2 and inhibiting E-cad expression.
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http://dx.doi.org/10.1016/j.canlet.2013.01.033DOI Listing
June 2013

Phase-transition induced changes in the electron-phonon coupling of all-trans-β-carotene.

Spectrochim Acta A Mol Biomol Spectrosc 2013 Mar 29;104:92-6. Epub 2012 Nov 29.

College of Physics, Jilin University, Changchun 130012, PR China.

The resonance Raman spectra of the fundamental, combination, and second harmonic modes around the C-C and C=C stretches of all-trans-β-carotene in 1,2-dichloroethane solution are obtained in the 323-83 K temperature range. The Raman scattering cross-section of the fundamentals in the liquid and solid phases generally increases as the temperature decreases, except for the liquid-solid phase transition, which exhibits a decreasing trend. The relative Raman intensities of the combination and harmonic modes of the CC bonds increase as the temperature decreases. The Raman bandwidths of the C=C bonds gradually become narrow but then appear a turning-point in the phase transition. The temperature-induced fundamental modes are analyzed using a coherent weakly damped electron-lattice vibration model and resonant Raman effects. The changes in the combination and harmonic modes are interpreted using the aforementioned model as well as the theory of electron-phonon interaction.
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http://dx.doi.org/10.1016/j.saa.2012.10.077DOI Listing
March 2013

Study of high-pressure Raman intensity behavior of aromatic hydrocarbons: benzene, biphenyl and naphthalene.

Spectrochim Acta A Mol Biomol Spectrosc 2012 Nov 7;97:526-31. Epub 2012 Jul 7.

State Key Laboratory of Superhard Materials, College of Physics, Jilin University, Changchun 130012, PR China.

Raman spectra of benzene, naphthalene and biphenyl have been taken up to a pressure of 13 GPa. The results for benzene and naphthalene indicate that the perturbation of inter-molecular π-π stacking effect on the Raman intensity is neglectable. For biphenyl, all the Raman peaks show intensity enhancement during the compression process, which indicates the planar intra-molecular aromatic conformation play an important role in the intensity increments of Raman bands. For three aromatic compounds, C-H stretching vibration bands located at about 3000 cm(-1) show intriguing intensity changes during compression account for the inter-molecular C-H⋯π interaction.
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http://dx.doi.org/10.1016/j.saa.2012.07.001DOI Listing
November 2012

Raman spectroscopy study on the ν1-2ν2 Fermi resonance of liquid carbon disulfide in binary solutions: effect of the weak hydrogen bond formation on the Fermi resonance.

Spectrochim Acta A Mol Biomol Spectrosc 2012 Oct 22;96:193-9. Epub 2012 May 22.

State Key Laboratory of Superhard Materials, College of Physics, Jilin University, Changchun 130012, PR China.

We have measured the Raman spectra of liquid CS(2) at different volume concentrations in CHCl(3) and CH(2)Cl(2) solutions. With decreasing the volume concentration of CS(2), a noticeable growth in the 2ν(2) band frequency was observed, while the ν(1) band location remained practically unchanged. This asymmetric wavenumber shift phenomenon of the Fermi doublet ν(1) and 2ν(2) of CS(2) has been ascribed to weak, non-conventional hydrogen bonds formed between the CS(2) and the solvent molecules. These weak hydrogen bonds were also responsible for significant decreases in the C-H bond symmetric stretching vibration band frequencies of CHCl(3) and CH(2)Cl(2). The values of the ν(1)-2ν(2) FR parameters of CS(2) in CH(2)Cl(2) and CHCl(3) at different volume concentrations were calculated according to the FR theory. The magnitude of the FR coupling coefficient W of CS(2) increases upon dilution with CH(2)Cl(2) and CHCl(3), indicating that the vibrational anharmonicity is relatively sensitive to variations in the weak hydrogen bonding. Compared with the changing tendencies of Fermi coupling coefficient W of CS(2) in CH(2)Cl(2) and CHCl(3) at different volume concentrations, we discussed the effect of the weak hydrogen bond formation on the FR and the asymmetric wavenumber shift phenomenon of the Fermi doublet ν(1) and 2ν(2) of CS(2).
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http://dx.doi.org/10.1016/j.saa.2012.05.024DOI Listing
October 2012

Detection of antibodies against major histocompatibility complex class I-related chain A in long-term renal graft recipients.

Exp Clin Transplant 2012 Jun;10(3):239-42

Department of Urology and Renal Transplantation, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, China.

Objectives: To determine the prevalence, allele specificity, and intensity of anti-MICA antibodies in long-term renal graft recipients and to investigate their association with impaired renal function.

Materials And Methods: Sixty-eight long-term (> 10 y) renal graft recipients were divided into 2 groups: (1) patients with impaired renal function (serum creatinine ≥ 2 mg/dL, n=6); (2) patients with normal renal function (serum creatinine < 176.8 μmol/L, n=62). Anti-MICA antibodies were tested using Luminex single antigen beads assays and the frequency, specificity, and intensity of these antibodies were compared between 2 patient groups.

Results: MICA antibodies were detected in 33% of impaired renal function patients and 15% of normal renal function patients (P > .05). Anti-MICA*027 antibodies were found in 11.76% of patients, whereas antibody to MICA*012 was found in 2.94% of patients. Interestingly, among all antibody specificities, MICA*001,*004, *007, *009, *012, and *018 were found more frequently in impaired renal function patients than in normal renal function patients. The peak mean fluorescence intensity levels of MICA antibodies in impaired renal function patients were significantly higher than those in normal renal function patients (P < .05).

Conclusions: Our data suggest that increased prevalence and intensity of anti-MICA antibodies are associated with impaired renal graft function in long-term renal graft recipients and some MICA antibodies might be more important than others in mediating graft rejection.
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http://dx.doi.org/10.6002/ect.2011.0103DOI Listing
June 2012

Stimulated Raman scattering of lattice translational modes in liquid heavy water.

Opt Lett 2012 Apr;37(8):1319-21

State Key Laboratory of Superhard Materials, College of Physics, Jilin University, Changchun 130012, China.

A study was conducted on stimulated Raman scattering (SRS) when laser-induced plasma is formed in heavy water by focusing an intense picosecond pulsed Nd:YAG laser beam with wavelength 532 nm at room temperature. An unexpected 280 cm(-1) low frequency SRS line attributed to the lattice translational modes is observed. This SRS line and the internal-mode SRS lines indicate that the ice VII structure is formed in heavy water under the condition of laser-induced shockwave production.
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http://dx.doi.org/10.1364/OL.37.001319DOI Listing
April 2012
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