Publications by authors named "Zixuan Li"

68 Publications

LARP7 ameliorates cellular senescence and aging by allosterically enhancing SIRT1 deacetylase activity.

Cell Rep 2021 Nov;37(8):110038

Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Department of Pediatric Cardiology, Xin Hua Hospital, School of Medicine, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200240, China. Electronic address:

Cellular senescence is associated with pleiotropic physiopathological processes, including aging and age-related diseases. The persistent DNA damage is a major stress leading to senescence, but the underlying molecular link remains elusive. Here, we identify La Ribonucleoprotein 7 (LARP7), a 7SK RNA binding protein, as an aging antagonist. DNA damage-mediated Ataxia Telangiectasia Mutated (ATM) activation triggers the extracellular shuttling and downregulation of LARP7, which dampens SIRT1 deacetylase activity, enhances p53 and NF-κB (p65) transcriptional activity by augmenting their acetylation, and thereby accelerates cellular senescence. Deletion of LARP7 leads to senescent cell accumulation and premature aging in rodent model. Furthermore, we show this ATM-LARP7-SIRT1-p53/p65 senescence axis is active in vascular senescence and atherogenesis, and preventing its activation substantially alleviates senescence and atherogenesis. Together, this study identifies LARP7 as a gatekeeper of senescence, and the altered ATM-LARP7-SIRT1-p53/p65 pathway plays an important role in DNA damage response (DDR)-mediated cellular senescence and atherosclerosis.
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http://dx.doi.org/10.1016/j.celrep.2021.110038DOI Listing
November 2021

Cellulose synthase-like protein OsCSLD4 plays an important role in the response of rice to salt stress by mediating abscisic acid biosynthesis to regulate osmotic stress tolerance.

Plant Biotechnol J 2021 Oct 18. Epub 2021 Oct 18.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.

Cell wall polysaccharide biosynthesis enzymes play important roles in plant growth, development and stress responses. The functions of cell wall polysaccharide synthesis enzymes in plant growth and development have been well studied. In contrast, their roles in plant responses to environmental stress are poorly understood. Previous studies have demonstrated that the rice cell wall cellulose synthase-like D4 protein (OsCSLD4) is involved in cell wall polysaccharide synthesis and is important for rice growth and development. This study demonstrated that the OsCSLD4 function-disrupted mutant nd1 was sensitive to salt stress, but insensitive to abscisic acid (ABA). The expression of some ABA synthesis and response genes was repressed in nd1 under both normal and salt stress conditions. Exogenous ABA can restore nd1-impaired salt stress tolerance. Moreover, overexpression of OsCSLD4 can enhance rice ABA synthesis gene expression, increase ABA content and improve rice salt tolerance, thus implying that OsCSLD4-regulated rice salt stress tolerance is mediated by ABA synthesis. Additionally, nd1 decreased rice tolerance to osmotic stress, but not ion toxic tolerance. The results from the transcriptome analysis showed that more osmotic stress-responsive genes were impaired in nd1 than salt stress-responsive genes, thus indicating that OsCSLD4 is involved in rice salt stress response through an ABA-induced osmotic response pathway. Intriguingly, the disruption of OsCSLD4 function decreased grain width and weight, while overexpression of OsCSLD4 increased grain width and weight. Taken together, this study demonstrates a novel plant salt stress adaptation mechanism by which crops can coordinate salt stress tolerance and yield.
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http://dx.doi.org/10.1111/pbi.13729DOI Listing
October 2021

Array-Structured Double-Ion Cooperative Adsorption Sites as Multifunctional Sulfur Hosts for Lithium-Sulfur Batteries with Low Electrolyte/Sulfur Ratio.

ACS Nano 2021 Oct 30;15(10):16322-16334. Epub 2021 Sep 30.

School of Chemistry, Chemical Engineering and Life Science, State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China.

Low electrolyte/sulfur ratio (E/S) is a crucial factor that promotes the development of lithium-sulfur batteries (LSBs) with desired energy density. However, it causes multiple problems, including a strong "shuttle effect" during both the cycle and storage process, and limited sulfur utilization. Herein, we develop a NaTiO (NTO) nanowire array as a multifunctional sulfur host to simultaneously tackle both the above problems. The synergistic coordination between Na and Ti cations in NTO can accelerate the conversion of soluble polysulfides (PSs) to insoluble sulfides and significantly enhance their adsorption. Therefore, accumulation of PSs, which is the primary cause of the "shuttle effect", can be avoided in two ways. One is fast conversion kinetics during cycles; another is strong PS adsorption, which can suppress the disproportionation of PSs during storage. The as-prepared array represents an easy-to-infiltrate structure with efficient electron transport that allows good wetting ability of the conductive surface toward the electrolyte. Therefore, it helps improve sulfur utilization that is mainly limited by the presence of unwetted conductive surface. Consequently, NTO/sulfur array cathodes exhibit high sulfur utilization and extended cycle- and shelf-lives at a low E/S (5:1). Our work suggests that array materials featuring cooperative multi-ion adsorption sites are promising hosts for LSBs.
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http://dx.doi.org/10.1021/acsnano.1c05536DOI Listing
October 2021

New azaphilones from mangrove endophytic fungus SCNU-F0040.

Nat Prod Res 2021 Sep 9:1-9. Epub 2021 Sep 9.

School of Chemistry, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou China.

Two new sclerotioramines ( and ) and a new natural product of sclerotioramine analog (), together with seven known compounds have been isolated from the mangrove endophytic fungus SCNU-F0040. Their structures were identified based on the 1 D, 2 D NMR and HRESIM spectra. The absolute configurations of new compounds were deduced by specific rotation data and electronic circular dichroism spectra. All the isolated new compounds were tested on anti-diabetes activity by using a-glucosidase inhibition assay and anti-inflammatory activity by using cyclooxygenase inhibition assay, respectively. Compounds and have a-glucosidase inhibition activity with IC values of 102.3 and 217.5 μM. Compound shows a moderate cyclooxygenase-2 inhibitory activity with an IC value of 47.8 μM.
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http://dx.doi.org/10.1080/14786419.2021.1959580DOI Listing
September 2021

PAQR9 regulates hepatic ketogenesis and fatty acid oxidation during fasting by modulating protein stability of PPARα.

Mol Metab 2021 Nov 30;53:101331. Epub 2021 Aug 30.

CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China. Electronic address:

Background: The cycle of feeding and fasting is fundamental to life and closely coordinated with changes of metabolic programs. During extended starvation, ketogenesis coupled with fatty acid oxidation in the liver supplies ketone bodies to extrahepatic tissues as the major form of fuel. In this study, we demonstrated that PAQR9, a member of the progesterone and adipoQ receptor family, has a regulatory role on hepatic ketogenesis.

Methods: We analyzed the phenotype of Paqr9-deleted mice. We also used biochemical methods to investigate the interaction of PAQR9 with PPARα and HUWE1, an E3 ubiquitin ligase.

Results: The expression of Paqr9 was decreased during fasting partly depending on PPARγ. The overall phenotype of the mice was not altered by Paqr9 deletion under normal chow feeding. However, fasting-induced ketogenesis and fatty acid oxidation were attenuated by Paqr9 deletion. Mechanistically, Paqr9 deletion decreased protein stability of PPARα via enhancing its poly-ubiquitination. PAQR9 competed with HUWE1 for interaction with PPARα, thus preventing ubiquitin-mediated degradation of PPARα.

Conclusion: Our study reveals that PAQR9 impacts starvation-mediated metabolic changes in the liver via post-translational regulation of PPARα.
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http://dx.doi.org/10.1016/j.molmet.2021.101331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452888PMC
November 2021

Hypericin Enhances Paclitaxel-Induced B16-F10 Cell Apoptosis by Activating a Cytochrome Release-Dependent Pathway.

Front Pharmacol 2021 4;12:652452. Epub 2021 Aug 4.

East China University of Science and Technology, Shanghai, China.

The enhanced inhibitory effect of paclitaxel (PTX) combined with hypericin (HY) on B16-F10 cells may be realized through the ROS-related cytochrome c release pathway. The apoptotic characteristics of the B16-F10 cells, such as DNA fragmentation, chromatin condensation, and apoptotic body formation, were all enhanced in the combined treatment group. Further investigation showed that the combination of paclitaxel and HY could increase the level of mitochondrial damage and the concentration of cytochrome , causing the expression of caspase-3 and the cleavage of PARP.. Compared with paclitaxel or HY alone, the level of reactive oxygen species (ROS) increased significantly, while glutathione reductase (GR) activity and intracellular glutathione (GSH) levels decreased significantly in the combination group.
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http://dx.doi.org/10.3389/fphar.2021.652452DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371448PMC
August 2021

Prognostic significance of excision repair cross complementation group 1 rs2298881 in patients with gastric cancer receiving platinum-based chemotherapy: A PRISMA-compliant meta-analysis.

Medicine (Baltimore) 2021 Aug;100(33):e26850

Department of Oncological Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Background: Gastric cancer (GC) is a strong cause of global cancer mortality. Nucleotide excision repair (NER) can modulate platinum-based chemotherapeutic efficacy by removing drug-produced DNA damage. Some studies have found a link between excision repair cross complementation group 1 (ERCC1) rs2298881, one gene in NER pathway, and response to chemotherapy. However, the results have been disputed.

Methods: We conducted a meta-analysis to reevaluate the association between polymorphisms of NER gene (ERCC1 rs2298881) and the clinical outcomes in gastric cancer patients receiving platinum-based chemotherapy. Searching PubMed, Web of Science, EMBASE, Google Scholar, and China National Knowledge Infrastructure, 2 independent searchers found all pertinent literatures up to May 1, 2021. We enrolled studies according to consistent selection criteria, extracted and vitrified data. Crude odds ratios (ORs) and hazard ratios (HRs) with 95% confidence interval (CI) were applied to evaluate the effect of ERCC1 rs2298881 on patients treated by platinum-based chemotherapy.

Results: By the data gathered from 6 independent studies, 1940 cases diagnosed with gastric cancer and treated with chemotherapy were included, containing 1208 Good-Responders and 732 Poor-Responders. With a comprehensive meta-analysis, we found that the patients with ERCC1 rs2298881A allele had a worse response to chemotherapy than those who with rs2298881C allele under allelic model (A vs C), with the pooled OR of 0.780 (95% CI: 0.611-0.996, P = .046). And our analysis indicated that AA genotype was associated with unfavorable overall survival (HR = 1.540, 95% CI = 1.106-2.144, P = .011) compared with CC genotype.

Conclusions: ERCC1 rs2298881 is suggested as a marker of clinical outcome in gastric cancer patients treated by platinum-based chemotherapy.
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http://dx.doi.org/10.1097/MD.0000000000026850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376342PMC
August 2021

Andrographolide Suppresses the Growth and Metastasis of Luminal-Like Breast Cancer by Inhibiting the NF-κB/miR-21-5p/PDCD4 Signaling Pathway.

Front Cell Dev Biol 2021 23;9:643525. Epub 2021 Jun 23.

School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, China.

Tumor growth and metastasis are responsible for breast cancer-related mortality. Andrographolide (Andro) is a traditional anti-inflammatory drug used in the clinic that inhibits NF-κB activation. Recently, Andro has been found in the treatment of various cancers. Andro inhibits breast cell proliferation and invasion and induces apoptosis activating various signaling pathways. Therefore, the underlying mechanisms with regard to the antitumor effects of Andro still need to be further confirmed. Herein, a MMTV-PyMT spontaneous luminal-like breast cancer lung metastatic transgenic tumor model was employed to estimate the antitumor effects of Andro on breast cancer . Andro significantly inhibited tumor growth and metastasis in MMTV-PyMT mice and suppressed the cell proliferation, migration, and invasion of MCF-7 breast cancer cells . Meanwhile, Andro significantly inhibited the expression of NF-κB, and the downregulated NF-κB reduced miR-21-5p expression. In addition, miR-21-5p dramatically inhibited the target gene expression of programmed cell death protein 4 (PDCD4). In the current study, we demonstrated the potential anticancer effects of Andro on luminal-like breast cancer and indicated that Andro inhibits the expression of miR-21-5p and further promotes PDCD4 NF-κB suppression. Therefore, Andro could be an antitumor agent for the treatment of luminal-like breast cancer in the clinic.
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http://dx.doi.org/10.3389/fcell.2021.643525DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261247PMC
June 2021

The relationship between maternal perfluoroalkylated substances exposure and low birth weight of offspring: a systematic review and meta-analysis.

Environ Sci Pollut Res Int 2021 Jul 9. Epub 2021 Jul 9.

Department of Occupational and Environmental Health, School of Public Health, Hebei Key Laboratory of Environment and Human Health, Hebei Medical University, Zhongshan East Road 361, Shijiazhuang, 050017, Hebei, People's Republic of China.

Some studies have shown that maternal perfluoroalkylated substances (PFAS) exposure may be associated with low birth weight (LBW) of offspring. We conducted a meta-analysis to assess the association between maternal PFASs exposure and LBW in offspring. The researchers searched PubMed, Science Direct, Scopus, Google Scholar, Web of Science, and Embase to find all the articles before October 2020. The Newcastle-Ottawa Scale was used to evaluate the quality of the studies. Finally, six articles were included for meta-analysis. Our meta-analysis showed no significant correlation between maternal perfluorooctanoic acid (PFOA) exposure and LBW of offspring: odds ratio (OR) = 0.90, 95% confidence interval (95% CI) = 0.80-1.01, with low heterogeneity (I = 18.4%, P = 0.289); there was a significant positive correlation between maternal perfluorooctane sulfonate (PFOS) exposure and LBW of offspring (OR = 1.32, 95% CI = 1.09-1.55) with no heterogeneity (I = 0.00%, P = 0.570). The grouping analysis of PFOS showed was a significant positive correlation between maternal PFOS exposure and LBW of offspring in American (OR = 1.44, 95% CI = 1.15-1.72). This study provided a systematic review and meta-analysis evidence for the relationship between maternal PFASs exposure and LBW of offspring through a small number of studies. Researchers should conduct further studies between different regions.
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http://dx.doi.org/10.1007/s11356-021-15061-4DOI Listing
July 2021

The association between maternal perfluoroalkyl substances exposure and early attention deficit hyperactivity disorder in children: a systematic review and meta-analysis.

Environ Sci Pollut Res Int 2021 Jul 9. Epub 2021 Jul 9.

Department of Occupational and Environmental Health, School of Public Health, Hebei Medical University, Hebei Key Laboratory of Environment and Human Health, Zhongshan East Road 361, Shijiazhuang, 050017, Hebei, People's Republic of China.

Some studies have shown that maternal exposure to perfluoroalkyl substances (PFASs) may be associated with early attention deficit hyperactivity disorder (ADHD) in children. The purpose of this systematic review and meta-analysis is to verify this association by reviewing existing studies and to provide a strong basis for preventing ADHD. The researchers searched electronic databases such as PubMed, Science Direct, Scopus, Google Scholar, Web of Science, and Embase for all studies published before October 2020. Finally, we included nine articles for analysis. Our meta-analysis showed that maternal exposure to PFASs was not significantly associated with the prevalence rate of early childhood ADHD (perfluorooctanoic acid (PFOA), odds ratio (OR) = 1.00, 95% confidence interval (95% CI) = 0.75-1.25; perfluorooctane sulfonate (PFOS), OR = 1.01, 95% CI = 0.88-1.14; perfluorohexane sulfonate (PFHxS), OR = 1.08, 95% CI = 0.80-1.09; perfluorononanoic acid (PFNA), OR = 1.13, 95% CI = 0.99-1.28; perfluorodecanoic acid (PFDA), OR = 1.23, 95% CI = 0.15-2.32). Due to significant heterogeneity, we subsequently performed subgroup analysis and sensitivity analysis. Through subgroup analysis, we found that PFOS concentration of children's blood and the prevalence rate of early childhood ADHD were statistically positively correlated, and there was also a positive correlation between PFOS exposure and the prevalence rate of early childhood ADHD in the America. Moreover, there was also a statistically positive correlation between PFNA concentration in maternal blood and the prevalence rate of early childhood ADHD. Sensitivity analysis showed that the final results did not change much, the sensitivity was low, and the results were relatively stable. In conclusion, a causal relationship between maternal PFASs exposure and ADHD in children was unlikely. Among them, PFOS, PFNA, and ADHD might have positive associations worthy of further investigation.
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http://dx.doi.org/10.1007/s11356-021-15136-2DOI Listing
July 2021

Transcriptome analysis provides insights into copper toxicology in piebald naked carp (Gymnocypris eckloni).

BMC Genomics 2021 Jun 5;22(1):416. Epub 2021 Jun 5.

College of Eco-Environmental Engineering, Qinghai University, Xining, 810016, China.

Background: Copper was used for many years in aquaculture operations as an effective algaecide or a parasite treatment of fish. It is an essential nutrient with numerous functions in organisms, but is toxic at high concentrations. However, the toxicity of copper to fish remains unclear. In this study, we used the piebald naked carp, Gymnocypris eckloni, as a model. RNA-seq data from different tissues, including gills, kidney, and liver, were used to investigate the underlying mechanism of copper toxicology in G. eckloni.

Results: We compared the transcriptomes from different tissues with different time durations of copper ion treatment. After 72 h copper ion treatment, the number of genes with different expression in gills and liver changed dramatically, but not in kidneys. In KEGG functional enrichment, the pattern of differentially expressed genes (DEGs) was also similar in the gills and liver. The most enriched pathway of DEGs was "Ribosome" in both tissues. Furthermore, we analyzed the expression levels of genes involved in oxidative stress response and protein synthesis using qPCR and RNA-seq data. Our results showed that several genes involved in oxidative stress response were up-regulated both in gills and liver. Up-regulation of these genes indicated that copper treatment caused oxidative stress, which is likely to result in ribosome damage. In addition, our results showed that the expression of Eef1b2, a transcription elongation factor, was decreased in the liver under oxidative stress, and the expression of translation initiation factors Eif4ebp1 and eIF2α, and elongation factor eEF2 was up-regulated. These results supported the idea that oxidative stress inhibits protein synthesis in cells.

Conclusions: Our results indicate that copper exposure caused different responses in different tissues, since the gene expression patterns changed substantially either in the gills or liver, while the effect on the kidney was relatively weak. Furthermore, our results indicated that the expression pattern of the genes involved in the ribosome, which is a complex molecular machine orchestrating protein synthesis in the cell, together with translation initiation factor and elongation factors, were affected by copper exposure both in the gills and liver of piebald naked carp. This result leads us to speculate that the downregulation of global protein synthesis is an acute response strategy of fish to metal-induced oxidative stress. Moreover, we speculate that this strategy not only exists in the selective translation of proteins but also exists in the specific translation of functional proteins in tissues and cells.
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http://dx.doi.org/10.1186/s12864-021-07673-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178853PMC
June 2021

Fluoride sensing performance of fluorescent NH-MIL-53(Al): 2D nanosheets 3D bulk.

Dalton Trans 2021 Jun;50(24):8540-8548

Key Laboratory of Environmental Optics and Technology, and Institute of Solid State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, P. R. China.

Due to their ultra-thin morphology, larger specific surface area and more exposed active sites, two-dimensional (2D) metal-organic framework (MOF) nanosheets can break the limitations of three-dimensional (3D) MOFs in sensitivity, response speed and the limit of detection for sensing applications. In this work, fluorescent NH2-MIL-53(Al) nanosheets were developed as a fluoride detection sensor compared with the 3D bulk counterpart. The morphological and structural characteristics of the obtained products were systematically characterized, and the favourable chemical and fluorescence stability of the NH2-MIL-53(Al) nanosheets were explored. The fluorescent NH2-MIL-53(Al) nanosheets showed high sensitivity, fast response speed (as short as 10 seconds), low limit of detection (15.2 ppb), and wide linear detection range (5-250 μM), and all performances were better than those of their bulk counterpart. In addition, the sensing mechanism was investigated to be based on the transformation of the NH2-MIL-53(Al) framework that induced the release of fluorescent ligands, resulting in an exceptionally enhanced fluorescence. This work highlights the advantages of 2D MOF nanosheets in fluorescence sensing applications.
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http://dx.doi.org/10.1039/d1dt00666eDOI Listing
June 2021

A high-performance free-standing Zn anode for flexible zinc-ion batteries.

Nanoscale 2021 Jun 31;13(22):10100-10107. Epub 2021 May 31.

School of Electronic and Computer Engineering, Peking University Shenzhen Graduate School, Shenzhen, China.

Zinc-ion batteries (ZIBs) have attracted significant attention owing to their high safety, high energy density, and low cost. ZIBs have been studied as a potential energy device for portable and flexible electronics. Here, a highly flexible free-standing Zn anode is fabricated using a simple spin-coating technique, and its application in ZIBs is demonstrated. The free-standing Zn anode precursor is formed by mixing Zn particles with carbon nanotubes and poly(vinylidene fluoride)-co-hexafluoropropylene (PVDF-HFP). The hexafluoropropylene group in PVDF-HFP improves the mechanical properties of the free-standing Zn anode, whereas the carbon nanotubes created percolation conduction in the composite electrode, leading to an increased electrical conductivity of the anode. Owing to the excellent electrical conductivity and high specific surface area of the free-standing Zn anode, ZIBs with high capacity, rate performance, and mechanical flexibility are achieved. The volumetric energy density of the ZIBs reaches 8.22 mW h cm with a battery thickness of 0.4 mm. This work demonstrates that free-standing Zn anodes are promising anodes for flexible ZIBs.
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http://dx.doi.org/10.1039/d1nr01266eDOI Listing
June 2021

The Multiple Roles of Ascorbate in the Abiotic Stress Response of Plants: Antioxidant, Cofactor, and Regulator.

Front Plant Sci 2021 12;12:598173. Epub 2021 Apr 12.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.

Ascorbate (ASC) plays a critical role in plant stress response. The antioxidant role of ASC has been well-studied, but there are still several confusing questions about the function of ASC in plant abiotic stress response. ASC can scavenge reactive oxygen species (ROS) and should be helpful for plant stress tolerance. But in some cases, increasing ASC content impairs plant abiotic stress tolerance, whereas, inhibiting ASC synthesis or regeneration enhances plant stress tolerance. This confusing phenomenon indicates that ASC may have multiple roles in plant abiotic stress response not just as an antioxidant, though many studies more or less ignored other roles of ASC in plant. In fact, ACS also can act as the cofactor of some enzymes, which are involved in the synthesis, metabolism, and modification of a variety of substances, which has important effects on plant stress response. In addition, ASC can monitor and effectively regulate cell redox status. Therefore, we believe that ASC has atleast triple roles in plant abiotic stress response: as the antioxidant to scavenge accumulated ROS, as the cofactor to involve in plant metabolism, or as the regulator to coordinate the actions of various signal pathways under abiotic stress. The role of ASC in plant abiotic stress response is important and complex. The detail role of ASC in plant abiotic stress response should be analyzed according to specific physiological process in specific organ. In this review, we discuss the versatile roles of ASC in the response of plants to abiotic stresses.
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http://dx.doi.org/10.3389/fpls.2021.598173DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072462PMC
April 2021

Nrf2 activation is involved in osteogenic differentiation of periodontal ligament stem cells under cyclic mechanical stretch.

Exp Cell Res 2021 06 15;403(2):112598. Epub 2021 Apr 15.

School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, China; Shandong Key Laboratory of Oral Tissue Regeneration, Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, China. Electronic address:

During orthodontic treatment, mechanical stretch serves a crucial function in osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Up-regulated reactive oxygen species (ROS) level is a result of cyclic mechanical stretch in many cell types. Nuclear factor erythroid-2-related factor-2 (Nrf2) is a master regulator in various antioxidants expression. However, it is not known whether cyclic mechanical stretch could induce the ROS generation in PDLSCs and whether Nrf2 participated in this process. The present study was aimed to investigate the role of Nrf2 in PDLSCs under cyclic mechanical stretch. Our results showed that cyclic mechanical stretch increased ROS level and the nuclear accumulation of Nrf2 during osteoblast differentiation. Knocking down Nrf2 by siRNA transfection increased ROS formation and suppressed osteogenic differentiation in PDLSCs. T-BHQ, a Nrf2 activator, promoted the osteogenic differentiation in PDLSCs under cyclic mechanical stretch, and improved the microstructure of alveolar bone during orthodontic tooth movement in rats by employing micro-CT system. Taken together, Nrf2 activation was involved in osteogenic differentiation under cyclic mechanical stretch in PDLSCs. T-BHQ could promote the osteogenic differentiation in vitro and in vivo, suggesting a promising option for the remodeling of the alveolar bone during orthodontic tooth movement.
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http://dx.doi.org/10.1016/j.yexcr.2021.112598DOI Listing
June 2021

Calcineurin Immune Signaling in Response to Zinc Challenge in the Naked Carp Gymnocypris eckloni.

Bull Environ Contam Toxicol 2021 May 23;106(5):792-798. Epub 2021 Mar 23.

College of Eco-Environmental Engineering, Qinghai University, Xining, 810016, China.

Zinc pollution impairs neural processes and protein function and also effects calcium-related transcriptional regulation and enzyme activity. In this study, we investigated pathways that potentially respond to calcium signaling under Zn stress. Specifically we measured relative expressions of GeCNAα, GeCNB, GeMT, GeTNF-α, GeIL-1β, and GeHsp90 in gills, livers, and kidneys of the indicator species Gymnocypris eckloni and found wide variation in their expression between tissues during the course of Zn exposure. Notably, GeCNAα, GeCNB, GeTNF-α, GeIL-1β, and GeMT were rapidly and strongly up-regulated in gills; GeIL-1β and GeHsp90 transcription was quickly induced in kidneys; and GeCNB, GeTNF-α, GeIL-1β, and GeHsp90 were most rapidly up-regulated in livers. GeCNAα and GeMT showed a contrasting late transcriptional up-regulation. These results suggest independent branches for chelation and immune responses during self-protection against Zn toxicity, and the immune response appears to be faster than metal chelation.
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http://dx.doi.org/10.1007/s00128-021-03178-8DOI Listing
May 2021

LARP7 Protects Against Heart Failure by Enhancing Mitochondrial Biogenesis.

Circulation 2021 May 5;143(20):2007-2022. Epub 2021 Mar 5.

Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Department of Pediatric Cardiology, Xin Hua Hospital, School of Medicine, Xin Hua Hospital, Shanghai Jiao Tong University, China (H.J.Y., F.Z., P.Y.Y., S.S.Z., Y.M.L., Z.L.G., Z.X.L., Y.J.X., Y.N.L., K.S., B.Z.).

Background: Heart failure (HF) is among the leading causes of morbidity and mortality, and its prevalence continues to rise. LARP7 (La ribonucleoprotein domain family member 7) is a master regulator that governs the DNA damage response and RNAPII (RNA polymerase II) pausing pathway, but its role in HF pathogenesis is incompletely understood.

Methods: We assessed LARP7 expression in human HF and in nonhuman primate and mouse HF models. To study the function of LARP7 in heart, we generated global and cardiac-specific knockout mice. We acutely abolished LARP7 in mature cardiomyocytes by Cas9-mediated somatic knockout. We overexpressed LARP7 in cardiomyocytes using adeno-associated virus serotype 9 and ATM (ataxia telangiectasia mutated protein) inhibitor. The therapeutic potential of LARP7-regulated pathways in HF was tested in a mouse myocardial infarction model.

Results: LARP7 was profoundly downregulated in failing human hearts and in nonhuman primate and murine hearts after myocardial infarction. Low LARP7 levels in failing hearts were linked to elevated reactive oxygen species, which activated the ATM-mediated DNA damage response pathway and promoted LARP7 ubiquitination and degradation. Constitutive knockout in mouse resulted in impaired mitochondrial biogenesis, myocardial hypoplasia, and midgestational lethality. Cardiac-specific inactivation resulted in defective mitochondrial biogenesis, impaired oxidative phosphorylation, elevated oxidative stress, and HF by 4 months of age. These abnormalities were accompanied by reduced SIRT1 (silent mating type information regulation 2 homolog 1) stability and deacetylase activity that impaired SIRT1-mediated transcription of genes for oxidative phosphorylation and energy metabolism and dampened cardiac function. Restoring LARP7 expression after myocardial infarction by either adeno-associated virus-mediated LARP7 expression or small molecule ATM inhibitor substantially improved the function of injured heart.

Conclusions: LARP7 is essential for mitochondrial biogenesis, energy production, and cardiac function by modulating SIRT1 homeostasis and activity. Reduction of LARP7 in diseased hearts owing to activation of the ATM pathway contributes to HF pathogenesis and restoring LARP7 in the injured heart confers myocardial protection. These results identify the ATM-LARP7-SIRT1 pathway as a target for therapeutic intervention in HF.
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http://dx.doi.org/10.1161/CIRCULATIONAHA.120.050812DOI Listing
May 2021

Perinatal exposure to BDE-47 exacerbated autistic-like behaviors and impairments of dendritic development in a valproic acid-induced rat model of autism.

Ecotoxicol Environ Saf 2021 Apr 4;212:112000. Epub 2021 Feb 4.

Department of Occupational and Environmental Health, School of Public Health, China Medical University, Shenyang, Liaoning, People's Republic of China. Electronic address:

Perinatal exposure to polybrominated diphenyl ethers (PBDEs) may be a potential risk factor for autism spectrum disorders (ASD). BDE-47 is one of the most common PBDEs and poses serious health hazards on the central nervous system (CNS). However, effects of perinatal exposure to BDE-47 on social behaviors and the potential mechanisms are largely unexplored. Thus, we aimed to investigate whether BDE-47 exposure during gestation and lactation led to autistic-like behaviors in offspring rats in the present study. Valproic acid (VPA), which is widely used to establish animal model of ASD, was also adopted to induce autistic-like behaviors. A battery of tests was conducted to evaluate social and repetitive behaviors in offspring rats. We found that perinatal exposure to BDE-47 caused mild autistic-like behaviors in offspring, which were similar but less severe to those observed in pups maternally exposed to VPA. Moreover, perinatal exposure to BDE-47 aggravated the autistic-like behaviors in pups maternally exposed to VPA. Abnormal dendritic development is known to be deeply associated with autistic-like behaviors. Golgi-Cox staining was used to observe the morphological characteristics of dendrites in the prefrontal cortex of pups. We found perinatal exposure to BDE-47 reduced dendritic length and complexity of branching pattern, and spine density in the offspring prefrontal cortex, which may contribute to autistic-like behaviors observed in the present study. Perinatal exposure to BDE-47 also exacerbated the impairments of dendritic development in pups maternally exposed to VPA. Besides, our study also provided the evidence that the inhibition of BDNF-CREB signaling, a key regulator of dendritic development, may be involved in the dendritic impairments induced by perinatal exposure to BDE-47 and/or VPA, and the consequent autistic-like behaviors.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112000DOI Listing
April 2021

Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D.

Mol Metab 2021 05 5;47:101182. Epub 2021 Feb 5.

CAS Key Laboratory of Nutrition, Metabolism, and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China. Electronic address:

Fat storage and mobilization in adipose tissue play a central role in energy metabolism and are directly linked to the development of obesity. Upon starvation, fat is mobilized from adipose tissue by lipolysis, a process by which triglycerides are hydrolyzed to free fatty acids to be used as an energy source in skeletal muscles and other tissues. However, how lipolysis is activated by starvation is not fully known. In this study, we demonstrate that PAQR11, a member of the progesterone and AdipoQ receptor family, regulates starvation-mediated lipolysis. Paqr11-deleted mice are resistant to high-fat diet-induced obesity. Paqr11 deletion promotes lipolysis in white adipose tissue, characterized by increased phosphorylations of hormone-sensitive lipase (HSL) and perilipin 1 (PLIN1) and elevated serum levels of glycerol and free fatty acids. PKA activity and cAMP levels in white adipose tissue are also increased by Paqr11 deletion, accompanied by accelerated protein degradation of phosphodiesterase 4D (PDE4D). Mechanistically, PAQR11 decreases the interaction of PDE4D with SKP1-CUL1-FBXO2 E3 ligase complex, thus modulating the polyubiquitination/degradation of PDE4D. Fasting decreases the expression of the Paqr11 gene, and starvation-induced lipolysis in white adipose tissue is enhanced by Paqr11 deletion, while insulin-mediated suppression of lipolysis is not affected. Collectively, these results reveal that PAQR11 regulates lipolysis of adipose tissue and affects high-fat diet-induced obesity.
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http://dx.doi.org/10.1016/j.molmet.2021.101182DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906896PMC
May 2021

Relative effectiveness of facemask therapy with alternate maxillary expansion and constriction in the early treatment of Class III malocclusion.

Am J Orthod Dentofacial Orthop 2021 Mar 22;159(3):321-332. Epub 2021 Jan 22.

Department of Orthodontics, School and Hospital of Stomatology, Shandong University, and Shandong Provincial Key Laboratory of Oral Tissue Regeneration, and Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, Shandong Province, China. Electronic address:

Introduction: This study aimed to investigate the relative efficacy of maxillary protraction combined with a modified alternate rapid maxillary expansion and constriction (Alt-RAMEC) protocol compared with conventional protocols in the early orthopedic treatment of skeletal Class III malocclusion.

Methods: A sample of 39 patients was divided into 3 groups on the basis of different interventions. Conventional facemask (FM) with splint-type intraoral devices was performed in the FM group (7 males and 5 females; mean age, 9.53 ± 1.37 years). Maxillary expansion with an activation rate of 0.5 mm/d (twice a day) followed by FM therapy was applied in the rapid maxillary expansion group (RME/FM) (6 males and 6 females; mean age, 9.31 ± 1.60 years). In the Alt-RAMEC/FM group (7 males and 8 females; mean age, 10.01 ± 1.31 years), Alt-RAMEC was started simultaneously and throughout the entire course of maxillary protraction, with repetitive alternations between activation and deactivation of expanders (0.5 mm/d for 7 days). The patients in all groups were instructed to wear FMs for a minimum of 12 h/d. Pretreatment and posttreatment lateral cephalograms were all traced and measured.

Results: The Alt-RAMEC group showed statistically more significant maxillary advancement than other groups (A-VRP, 3.87 mm vs 3.04 mm [RME/FM], vs 2.04 mm [FM]; P <0.05). Analysis of variance did not reveal significant intergroup differences in palatal plane angulation changes (P >0.05). No pronounced mandibular clockwise rotations were noted in the Alt-RAMEC/FM group with distinct intergroup differences (P <0.05). There were more skeletal effects (88.7%) during overjet correction in the Alt-RAMEC/FM protocol.

Conclusions: A combination of the modified Alt-RAMEC protocol with FM revealed more favorable skeletal effects compared with FM and RME/FM protocols in treating prepubertal patients with maxillary deficiency.
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http://dx.doi.org/10.1016/j.ajodo.2019.12.028DOI Listing
March 2021

Highly fluorescent scandium-tetracarboxylate frameworks: selective detection of nitro-aromatic compounds, sensing mechanism, and their application.

Dalton Trans 2020 Dec 25;49(48):17737-17744. Epub 2020 Nov 25.

State Key Laboratory of Sensor Technology, and Institute of Solid State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, P. R. China.

Recently, exploring new luminescent metal-organic frameworks (LMOFs) to selectively detect nitro-aromatic compounds (NACs) has been a hot topic of research. Simultaneously, it is still a challenging issue to understand the sensing mechanism of luminescent MOFs interacting with NACs at the molecular level. In this work, highly fluorescent Sc-tetracarboxylate frameworks (Sc-EBTC) have been successfully synthesized through a solvothermal method. The as-prepared Sc-EBTC crystals have good thermal stability, chemical stability as well as strong fluorescence (λ = 320 nm and λ = 400 nm), and they can detect various NACs rapidly (as short as 30 s), selectively and efficiently by the "turn-off" fluorescence mechanism. The detection limits of Sc-EBTC toward 2,4-DNP and 4-NP are quantified to be 5.71 ppb and 6.26 ppb, respectively. Furthermore, to better understand the sensing mechanism, we attempt to use solid-state NMR and X-ray photoelectron spectroscopy to vividly characterize the charge transfer caused by the interaction between NAC molecules and the MOF at the molecular level. Additionally, test strips were made successfully for the practical detection of the NACs. This study demonstrates that the MOF constructed from the HEBTC ligands might be a promising candidate for the detection of trace NACs.
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http://dx.doi.org/10.1039/d0dt03781hDOI Listing
December 2020

Bivalirudin vs. Heparin on Radial Artery Thrombosis during Transradial Coronary Intervention: An Optical Coherence Tomography Study.

J Interv Cardiol 2020 26;2020:7905021. Epub 2020 Sep 26.

Department of Cardiology, Beijing Luhe Hospital, Capital Medical University, Beijing 100000, China.

Objectives: This study aimed to evaluate the antithrombotic efficacy between bivalirudin and unfractionated heparin (UFH) on radial artery thrombosis (RAT) during transradial coronary intervention (TRI) by optical coherence tomography (OCT).

Methods And Results: We consecutively reviewed a total of 307 patients who underwent radial artery OCT inspection after TRI in our centre from October 2017 to January 2019; afterwards, 211 screened patients were divided into the UFH group ( = 144) and the bivalirudin group ( = 67) according to their anticoagulation strategy during TRI. The thrombosis in the radial artery was observed in 51 cases (24.17%) with a median thrombus volume of 0.054 mm (0.024, 0.334) and median thrombus score of 7 (4, 15). Thrombus occurred in 28 cases in the bivalirudin group with an incidence of 41.8%, which was significantly higher than that in the UFH group ( = 23, 16.0%, < 0.001). This difference was even more remarkable after propensity score matching (bivalirudin group  = 22, 42.3% vs. UHF group  = 11, 13.9%, < 0.001). Multivariate logistic analysis revealed that bivalirudin increased the RAT risk by 3.872 times (95% CI 2.006-8.354, < 0.001) after adjustment for the other predictors.

Conclusion: In this present study, the use of bivalirudin was associated with a higher risk of RAT than UFH. It highlighted UFH should be a more considerable choice to prevent radial artery access thrombosis in TRI.
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http://dx.doi.org/10.1155/2020/7905021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533783PMC
March 2021

L ARP7 Is a BRCA1 Ubiquitinase Substrate and Regulates Genome Stability and Tumorigenesis.

Cell Rep 2020 07;32(4):107974

Department of Pediatric Cardiology, Xin Hua Hospital, School of Medicine, Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

Attenuated DNA repair leads to genomic instability and tumorigenesis. BRCA1/BARD1 are the best-known tumor suppressors that promote homology recombination (HR) and arrest cell cycle. However, it remains ambiguous whether and how their E3 ligase activity regulates HR. Here, we demonstrate that upon genotoxic stress, BRCA1 together with BARD1 catalyzes the K48 polyubiquitination on LARP7, a 7SK RNA binding protein known to control RNAPII pausing, and thereby degrades it through the 26S ubiquitin-proteasome pathway. Depleting LARP7 suppresses the expression of CDK1 complex, arrests the cell at the G2/M DNA damage checkpoint, and reduces BRCA2 phosphorylation, which thereby facilitates RAD51 recruitment to damaged DNA to enhance HR. Importantly, LARP7 depletion observed in breast cancer patients leads to chemoradiotherapy resistance both in vitro and in vivo. Altogether, this study unveils a mechanism by which BRCA1/BARD1 control HR and cell cycle, and highlights LARP7 as a potential target for cancer prevention and therapy.
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http://dx.doi.org/10.1016/j.celrep.2020.107974DOI Listing
July 2020

Bio-inspired porous helical carbon fibers with ultrahigh specific surface area for super-efficient removal of sulfamethoxazole from water.

J Colloid Interface Sci 2020 Oct 3;578:304-314. Epub 2020 Jun 3.

Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, PR China. Electronic address:

Helical carbon fibers (HCFs) are a new kind of fascinating carbon material, and have caused much attention for their distinctive features, diversified novel properties, and applications. However, the application of HCFs still faces a series of barriers, especially in the repetitive preparation of HCFs. In this paper, we initially report the synthesis of the HCFs with ultrahigh specific surface area (3089 m g) by a bio-template process using the high purified spiral vessels (SVs) as the template. The helical structure with the ultrahigh specific surface area can efficaciously shorten the pathway for antibiotics diffusion, and the high content of nanopores (1 to 3 nm) not only guarantees the accessibility of the surface for antibiotics storage but also easily provides approachable channels for antibiotics transmission. The highest adsorption capacity for sulfamethoxazole (SMX) is 1091 mg/g at pH 6.0 ± 0.1 with a stable temperature of 20 °C when the initial SMX concentration is 80 mg/L. This study motivates a new bio-inspired design for preparing the high purified HCFs with a simple bio-template method. The results show that the porous HCFs are a new kind of ultrahigh adsorption material for the removal of SMX in aqueous solution and can be used in new technological applications.
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http://dx.doi.org/10.1016/j.jcis.2020.05.117DOI Listing
October 2020

Polyacrylic acid assisted synthesis of free-standing MnO/CNTs cathode for Zinc-ion batteries.

Nanotechnology 2020 Sep 1;31(37):375401. Epub 2020 Jun 1.

School of Electronic and Computer Engineering, Peking University Shenzhen Graduate School, Shenzhen 518055, People's Republic of China.

Rechargeable aqueous zinc-ion batteries (ZIBs) have attracted significant attention due to the distinguishing characteristics of zinc metal, including its low price, abundance in earth, safety and high theoretical specific capacity of 820 mAh g. Manganese dioxide (MnO) is a promising cathode for ZIBs due to high theoretical specific capacity, high discharge voltage plateau, cost-effectiveness and nontoxicity. However, the low electronic conductivity and volumetric changes during electrochemical cycling hinder its practical utilization. Herein, we demonstrate a polyacrylic acid (PAA)-assisted assembling strategy to fabricate freestanding and flexible MnO/carbon nanotube/PAA (MnO/CNT/PAA) cathodes for ZIBs. PAA plays an important role in providing excellent mechanical properties to the free-standing electrode. Moreover, the presence of CNT forms an electron conductive network, and the porous structure of MnO/CNT/PAA electrode accommodates the volumetric variations of MnO during charge/discharge cycling. The as-fabricated quasi-solid-state Zn-MnO/CNT/PAA battery delivers a high charge storage capacity of 302 mAh g at 0.3 A g and retains 82% of the initial capacity after 1000 charge/discharge cycles at 1.5 A g. The calculated volumetric energy density of Zn-MnO/CNT/PAA battery is 8.5 mW h cm (with a thickness of 0.08 cm), which is significantly higher than the reported alkali-ion batteries (1.3 mW h cm) and comparable to supercapacitors (6.8 mW h cm) and Ni-Zn batteries (7.76 mW h cm). The current work demonstrates that free-standing MnO/CNT/PAA composite is a promising cathode for ZIBs.
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http://dx.doi.org/10.1088/1361-6528/ab9866DOI Listing
September 2020

Downregulation of MicroRNA-551b Correlates With Dissemination of Human Oral Squamous Cell Carcinoma.

J Oral Maxillofac Surg 2020 Sep 11;78(9):1538-1545. Epub 2020 Apr 11.

Associate Professor, Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. Electronic address:

Purpose: Altered expression of microRNAs contributes to invasion and metastasis of many human cancers; however, the importance of microRNAs in head and neck cancers remains to be elucidated. In this study, we examined whether altered microRNA (miR)-551b expression correlated with invasive phenotypes of human oral squamous cell carcinoma (OSCC) in vivo and in vitro.

Materials And Methods: Real-time polymerase chain reaction was used to detect the expression level of miR-551b in 71 OSCC tissues with lymph node metastasis and 50 nonmetastatic OSCC tissues. We also constructed miR-551b mimic-transfected cell lines HN4 and HN12. The effects of overexpressing miR-551b on the proliferation, migration, and invasion of OSCC cells were examined using Cell Counting Kit 8 (Dojindo, Kumamoto, Japan), plate clone formation, wound healing, and Transwell invasion experiments (Corning, Corning, NY). The association between clinical pathologic parameters and the expression level of miR-551b was analyzed using Kaplan-Meier survival analysis.

Results: The expression of miR-551b measured 0.33 ± 0.11 in the 71 OSCC tissues with lymph node metastasis versus 0.54 ± 0.06 in the 50 tissues with non-lymph node metastasis (P = .021). Regarding OSCC patients, the expression of miR-551b negatively correlated with patients' overall survival (P = .035). The ectopic expression of miR-551b inhibited the invasion and migration of OSCC cells.

Conclusions: This is the first report showing that reduced miR-551b expression may be an event leading to OSCC invasion and metastasis.
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http://dx.doi.org/10.1016/j.joms.2020.03.044DOI Listing
September 2020

Proteome Analysis in a Mammalian Cell line Reveals that PLK2 is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis.

Viruses 2020 03 28;12(4). Epub 2020 Mar 28.

Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, No. 9 Shuguang Garden Middle Road, Haidian District, Beijing 100097, China.

Avian metapneumovirus subtype C (aMPV/C) causes an acute respiratory disease that has caused serious economic losses in the Chinese poultry industry. In the present study, we first explored the protein profile in aMPV/C-infected Vero cells using iTRAQ quantitative proteomics. A total of 921 of 7034 proteins were identified as significantly altered by aMPV/C infection. Three selected proteins were confirmed by Western blot analysis. Bioinformatics GO analysis revealed multiple signaling pathways involving cell cycle, endocytosis, and PI3K-Akt, mTOR, MAPK and p53 signaling pathways, which might participate in viral infection. In this analysis, we found that PLK2 expression was upregulated by aMPV/C infection and investigated whether it contributed to aMPV/C-mediated cellular dysfunction. Suppressing PLK2 attenuated aMPV/C-induced reactive oxygen species (ROS) production and p53-dependent apoptosis and reduced virus release. These results in a mammalian cell line suggest that high PLK2 expression correlates with aMPV/C-induced apoptosis and viral replication, providing new insight into the potential avian host cellular response to aMPV/C infection and antiviral targets.
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http://dx.doi.org/10.3390/v12040375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232392PMC
March 2020

Simultaneous retrieval of particle size and refractive index by extended interferometric particle imaging technique.

Opt Express 2020 Jan;28(2):2192-2200

A method is presented for simultaneously inferring both the refractive index and the size of a particle with extended interferometric particle imaging technique. The optical system of extended IPI with opposite two-sheet illumination at dual scattering angles is laid out for the experiment. The size of a particle is evaluated by the interference fringe recorded at the scattering angle of 90°, which is from the two reflected lights with two counter-propagating sheet illuminations. And then the refractive index is calculated by the fringe pattern recorded in the side scattering angle region with one of two-sheet illumination when combined with droplet size determined. Experiments on the polystyrene microsphere and water droplet suggest that the method presented herein is promising for many relevant applications, such as fuel combustion and environmental monitoring, in accurately measuring both the particle size and its refractive index.
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http://dx.doi.org/10.1364/OE.382512DOI Listing
January 2020

One-step synthesis of MnO/PPy nanocomposite as a high-performance cathode for a rechargeable zinc-ion battery and insight into its energy storage mechanism.

Nanoscale 2020 Feb 5;12(6):4150-4158. Epub 2020 Feb 5.

School of Electronic and Computer Engineering, Peking University Shenzhen Graduate School, Shenzhen 518055, PR China.

Rechargeable aqueous zinc-ion batteries (ZIBs) have attracted significant attention in the energy storage field. Manganese-based materials are the most promising cathode materials for ZIBs but they suffer from low electronic conductivity. Herein, a high-performance cathode for ZIBs based on nanocomposites consisting of mixed-valence manganese dioxide (Mn III and IV) and polypyrrole (MnO/PPy) is prepared through an efficient one-step organic/inorganic interface redox reaction. The role of polypyrrole (PPy) in the MnO/PPy cathode is elaborated. It not only provides an effective conductive network for MnO but also contributes to the capacity of the composite. By optimizing the amount of PPy, the MnO/PPy composite with 12 wt% PPy exhibits the highest capacity. As a result, the corresponding Zn-MnO/PPy battery delivers a high capacity (302.0 mA h g at 0.15 A g), excellent rate performance (159.9 mA h g at 3 A g) and superior cycling stability. Furthermore, the results of ex situ characterization analysis reveal that H and Zn insertion/extraction both occur in MnO/PPy particles during the discharging/charging process, while only Zn insertion/extraction occurs in the PPy electrode. This work develops an efficient one-step synthesis method for large scale production of manganese-based materials/conducting polymers as the cathode for ZIB application, and provides an insight into its energy storage mechanism.
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http://dx.doi.org/10.1039/c9nr09870dDOI Listing
February 2020
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