Publications by authors named "Ziwen Yuan"

26 Publications

  • Page 1 of 1

Sheng Mai San ameliorated heat stress-induced liver injury via regulating energy metabolism and AMPK/Drp1-dependent autophagy process.

Phytomedicine 2021 Dec 31;97:153920. Epub 2021 Dec 31.

Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070, China. Electronic address:

Background: Liver damage is one of the most common complications in humans and animals after heat stress (HS). Sheng Mai San (SMS), a traditional Chinese medicine prescription that originated in the Jin Dynasty, exert a therapeutic effect on HS. However, how SMS prevents liver injury after heat exposure remains unknown.

Purpose: This study aimed to investigate the pharmacological effect and molecular mechanisms of SMS on HS-induced liver injury.

Study Design: A comprehensive strategy via incorporating pharmacodynamics, targeted metabolomics, and molecular biology technology was adopted to investigate energy metabolism changes and the therapeutic mechanisms of SMS in HS-induced rat liver injury.

Methods: First, Sprague-Dawley rats were subjected to HS (38 °C/ 75% RH/ 2 h/ day) for 7 consecutive days to establish the HS model, and SMS was given orally for treatment 2 h before heat exposure. Thereafter, liver function and pathological changes in liver tissue were evaluated. Finally, the underlying mechanisms of SMS were determined using targeted energy metabolomics to comprehensively analyze the metabolic pathways and were further verified through Western-blot and qRT-PCR assays.

Results: Our results showed that SMS alleviated HS-induced liver dysfunction by reducing the alanine aminotransferase (ALT), aspartate aminotransferase (AST), and AST/ALT ratios in serum and improving hepatic pathological damage. Meanwhile, SMS suppressed inflammatory response, oxidative injury, and overexpression of heat shock proteins in liver tissue after heat exposure. With the help of targeted energy metabolomics, we found that SMS could effectively regulate glycolysis and tricarboxylic acid (TCA) cycle to relieve energy metabolism disorder. Furthermore, we confirmed that SMS can facilitate the phosphorylation of AMP-activated protein kinase (AMPK) to maintain mitochondrial homeostasis through a dynamin protein 1 (Drp1)-dependent mitophagy process.

Conclusion: On the basis of energy metabolomics, the present study for the first time systematically illustrated the protective effect of SMS on HS-induced liver injury, and preliminarily confirmed that an AMPK-mediated Drp1-dependent mitophagy and mitochondria rebuilding process plays an important role in SMS intervention on HS-induced rat liver. Together, our study lends further support to the use of SMS in treating HS condition.
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http://dx.doi.org/10.1016/j.phymed.2021.153920DOI Listing
December 2021

Effect of BCI-Controlled Pedaling Training System With Multiple Modalities of Feedback on Motor and Cognitive Function Rehabilitation of Early Subacute Stroke Patients.

IEEE Trans Neural Syst Rehabil Eng 2021 21;29:2569-2577. Epub 2021 Dec 21.

Brain-computer interfaces (BCIs) are currently integrated into traditional rehabilitation interventions after stroke. Although BCIs bring many benefits to the rehabilitation process, their effects are limited since many patients cannot concentrate during training. Despite this outcome post-stroke motor-attention dual-task training using BCIs has remained mostly unexplored. This study was a randomized placebo-controlled blinded-endpoint clinical trial to investigate the effects of a BCI-controlled pedaling training system (BCI-PT) on the motor and cognitive function of stroke patients during rehabilitation. A total of 30 early subacute ischemic stroke patients with hemiplegia and cognitive impairment were randomly assigned to the BCI-PT or traditional pedaling training. We used single-channel Fp1 to collect electroencephalography data and analyze the attention index. The BCI-PT system timely provided visual, auditory, and somatosensory feedback to enhance the patient's participation to pedaling based on the real-time attention index. After 24 training sessions, the attention index of the experimental group was significantly higher than that of the control group. The lower limbs motor function (FMA-L) increased by an average of 4.5 points in the BCI-PT group and 2.1 points in the control group (P = 0.022) after treatments. The difference was still significant after adjusting for the baseline indicators ( β = 2.41 , 95%CI: 0.48-4.34, P = 0.024). We found that BCI-PT significantly improved the patient's lower limb motor function by increasing the patient's participation. (clinicaltrials.gov: NCT04612426).
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http://dx.doi.org/10.1109/TNSRE.2021.3132944DOI Listing
January 2022

Thermo-osmosis-Coupled Thermally Regenerative Electrochemical Cycle for Efficient Lithium Extraction.

ACS Appl Mater Interfaces 2021 Feb 26;13(5):6276-6285. Epub 2021 Jan 26.

School of Chemical and Biomolecular Engineering, The University of Sydney, Camperdown, NSW 2006, Australia.

Lithium (Li) production based on the soda evaporation process is time-consuming and unsustainable. The emerging electrochemical Li extraction is time-efficient but requires high-concentration Li sources and significant electrical energy input. Here, we demonstrate a fast, energy-saving, and environment-friendly Li production process by coupling a thermally regenerative electrochemical cycle (TREC) using lithium manganese oxide (LMO) and nickel hexacyanoferrate (NiHCF) electrodes with poly(vinylidene fluoride) membrane-based thermo-osmosis (denoted as TO-TREC). The characterization of LMO and NiHCF electrodes confirmed that the relatively high temperature of TO-TREC has negligible adverse effects on the ion intercalation in LMO and NiHCF electrodes. The LMO/NiHCF pair has a positive temperature coefficient of 0.843 mV K. In the TO-TREC process, Li ions are selectively extracted from a Li-containing brine warmed by low-grade heat and then released into a room-temperature recovery solution such as LiCl with a production rate of 50-60 mmol Li m h. Li source solutions are concentrated by thermo-osmosis simultaneously, making it possible to utilize previously unusable Li-containing sources, such as concentrated brines from desalination plants and industrial effluents. Besides, the TREC harvests thermal energy from the heated brine, saving >20% of electrical energy compared to conventional electrochemical methods. The new process shows the potential to meet the growing global Li demands for many applications.
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http://dx.doi.org/10.1021/acsami.0c20464DOI Listing
February 2021

One-Dimensional van der Waals Heterostructures as Efficient Metal-Free Oxygen Electrocatalysts.

ACS Nano 2021 Feb 25;15(2):3309-3319. Epub 2021 Jan 25.

School of Chemical and Biomolecular Engineering, The University of Sydney, Darlington 2006, NSW Australia.

Two-dimensional covalent organic frameworks (2D-COFs) may serve as an emerging family of catalysts with well-defined atomic structures. However, the severe stacking of 2D nanosheets and large intrinsic bandgaps significantly impair their catalytic performance. Here, we report coaxial one-dimensional van der Waals heterostructures (1D vdWHs) comprised of a carbon nanotube (CNT) core and a thickness tunable thienothiophene-pyrene COF shell using a solution-based wrapping method. Density functional theory calculations and and spectroscopic analysis indicate that carbon-sulfur regions in thienothiophene groups in the COF serve as an active catalytic site for oxygen reduction and evolution reactions. The coaxial structure enables -doping from the CNT core to the COF shell, which is controllable by varying COF shell thickness. The charge transfer from CNTs lowers COF's bandgap and work function, which reduces the charge transfer barrier between the active catalytic sites and adsorbed oxygen intermediates, resulting in dramatically enhanced catalytic activity. The 1D vdWHs were applied as a bifunctional oxygen electrocatalyst in rechargeable zinc-air batteries, delivering a high specific capacity of 696 mAh g under a high current density of 40 mA cm and excellent cycling stability. The 1D vdWHs based on the coaxial structure of 2D COF wrapped around CNT cores may be further used as versatile building units to create multidimensional vdWHs for exploring fundamental physics and chemistry as well as practical applications in electrochemistry, electronics, photonics, and beyond.
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http://dx.doi.org/10.1021/acsnano.0c10242DOI Listing
February 2021

Intrinsic Activity of Metal Centers in Metal-Nitrogen-Carbon Single-Atom Catalysts for Hydrogen Peroxide Synthesis.

J Am Chem Soc 2020 12 17;142(52):21861-21871. Epub 2020 Dec 17.

School of Chemical and Biomolecular Engineering, The University of Sydney, Darlington, New South Wales 2006, Australia.

Metal-nitrogen-carbon (M-N-C) single-atom catalysts (SACs) show high catalytic activity for many important chemical reactions. However, an understanding of their intrinsic catalytic activity remains ambiguous because of the lack of well-defined atomic structure control in current M-N-C SACs. Here, we use covalent organic framework SACs with an identical metal coordination environment as model catalysts to elucidate the intrinsic catalytic activity of various metal centers in M-N-C SACs. A pH-universal activity trend is discovered among six 3d transition metals for hydrogen peroxide (HO) synthesis, with Co having the highest catalytic activity. Using density functional calculations to access a total of 18 metal species, we demonstrate that the difference in the binding energy of O* and HOOH* intermediates ( ) on single metal centers is a reliable thermodynamic descriptor to predict the catalytic activity of the metal centers. The predicted high activity of Ir centers from the descriptor is further validated experimentally. This work suggests a class of structurally defined model catalysts and clear mechanistic principles for metal centers of M-N-C SACs in HO synthesis, which may be further extendable to other reactions.
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http://dx.doi.org/10.1021/jacs.0c10636DOI Listing
December 2020

Screening study of blood-supplementing active components in water decoction of processed with yellow rice wine based on response surface methodology.

Pharm Biol 2020 Dec;58(1):1167-1176

College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, P. R. China.

Context: (Oliv.) Diels (Apiaceae) (syn. Maxim var. Oliver) processed with yellow rice wine (WAS) has a blood-supplementing effect.

Objective: To establish an optimal technology for preparing water decoction of WAS (WASD), and screen blood-supplementing fractions.

Materials And Methods: Ferulic acid and crude polysaccharide were used in optimizing the preparation technology for WASD through response surface methodology. The independent variables were liquid-solid ratio, soaking time, and extraction time. Eighty Kunming mice were randomly divided into normal control, model, and six intervention groups ( = 10). The intervention groups were given different WASD fractions by gavage (5 or 10 g/kg). The model intervention groups received acetylphenyl hydrazine (subcutaneous injection) and cyclophosphamide (intraperitoneal injection). Duration of study, 9 days. The components of blood-supplementing fractions were analyzed.

Results: The optimum extraction parameters were liquid-solid ratio, 7.69:1 mL/g; soaking time, 119.78 min; and extraction time, 143.35 min. The optimal OD value was 0.8437. RBC, WBC, and Hb in the water fraction (5, 10 g/kg) and -butanol fraction (10 g/kg) intervention groups increased significantly compared with the model group ( < 0.05). Polysaccharide and caffeic acid contents of water fraction were 252.565 and 0.346 μg/mg, respectively; ferulic acid was not detected. Caffeic acid and ferulic acid contents of -butanol fraction were 1.187 and 0.806 μg/mg, respectively, polysaccharide was not detected.

Conclusions: The optimum preparation technology of WASD was obtained, and the water, -butanol fractions were blood-supplementing fractions. This study provides a theoretical foundation for further application of WAS in the pharmaceutical industry.
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http://dx.doi.org/10.1080/13880209.2020.1844760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877401PMC
December 2020

2D Material Based Advanced Membranes for Separations in Organic Solvents.

Small 2020 12 20;16(50):e2003400. Epub 2020 Nov 20.

School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, NSW, 2006, Australia.

2D materials have shown high potentials for fabricating next-generation membranes. To date, extensive studies have focused on the applications of 2D material membranes in gas and aqueous media. Recently, compelling opportunities emerge for 2D material membranes in separation applications in organic solvents because of their unique properties, such as ultrathin mono- to few-layers, outstanding chemical resistance toward organic solvents. Hence, this review aims to provide a timely overview of the current state-of-the-art of 2D material membranes focusing on their applications in organic solvent separations. 2D material membranes fabricated using graphene materials and a few representative nongraphene-based 2D materials, including covalent organic frameworks and MXenes, are summarized. The key membrane design strategies and their effects on separation performances in organic solvents are also examined. Last, several perspectives are provided in terms of the critical challenges for 2D material membranes, including standardization of membrane performance evaluation, improving understandings of separation mechanisms, managing the trade-off of permeability and selectivity, issues related to application versatility, long-term stability, and fabrication scalability. This review will provide a useful guide for researchers in creating novel 2D material membranes for advancing new separation techniques in organic solvents.
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http://dx.doi.org/10.1002/smll.202003400DOI Listing
December 2020

Toward Flexible Zinc-Ion Hybrid Capacitors with Superhigh Energy Density and Ultralong Cycling Life: The Pivotal Role of ZnCl Salt-Based Electrolytes.

Angew Chem Int Ed Engl 2021 Jan 3;60(2):990-997. Epub 2020 Nov 3.

School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, New South Wales, 2006, Australia.

Zinc ion hybrid capacitors (ZIHCs) are promising energy storage devices for emerging flexible electronics, but they still suffer from trade-off in energy density and cycling life. Herein, we show that such a dilemma can be well-addressed by deploying ZnCl based electrolytes. Combining experimental studies and density functional theory (DFT) calculations, for the first time, we demonstrate an intriguing chloride ion (Cl ) facilitated desolvation mechanism in hydrated [ZnCl] (H O) (with n=1-6) clusters. Based on this mechanism, a water-in-salt type hydrogel electrolyte filled with ZnCl was developed to concurrently improve the energy storage capacity of porous carbon materials and the reversibility of Zn metal electrode. The resulting ZIHCs deliver a battery-level energy density up to 217 Wh kg at a power density of 450 W kg , an unprecedented cycling life of 100 000 cycles, together with excellent low-temperature adaptability and mechanical flexibility.
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http://dx.doi.org/10.1002/anie.202012030DOI Listing
January 2021

Viscosity sensitive near-infrared fluorescent probes based on functionalized single-walled carbon nanotubes.

Chem Commun (Camb) 2020 Jul 23;56(59):8301-8304. Epub 2020 Jun 23.

Guangdong Institute of Microbiology, Guangdong Academy of Sciences, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070, China.

A viscosity-sensitive fluorescent probe is demonstrated by grafting a rotatable 4-N,N'-dimethylaniline group on (6,5) single-walled carbon nanotubes. The rotation of the grafted group is constricted by solution viscosity, causing changes in the photoluminescence behaviors of the nanotubes. This enables a highly sensitive fluorescent probe for determining solution ratiometric viscosity in the biologically transparent second near-infrared region.
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http://dx.doi.org/10.1039/d0cc02813dDOI Listing
July 2020

Mechanism of Huang-lian-Jie-du decoction and its effective fraction in alleviating acute ulcerative colitis in mice: Regulating arachidonic acid metabolism and glycerophospholipid metabolism.

J Ethnopharmacol 2020 Sep 14;259:112872. Epub 2020 May 14.

Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China. Electronic address:

Ethnopharmacological Relevance: Huang-lian-Jie-du decoction (HLJDD) is a traditional Chinese medicine prescription for clearing away heat, purging fire and detoxifying, which can be used to treat sepsis, stroke, Alzheimer's disease and gastrointestinal diseases. Our previous studies have shown that HLJDD can effectively alleviate acute ulcerative colitis (UC) in mice, and its n-butanol fraction (HLJDD-NBA) is the effective fraction. The aim of this study is to further investigate the mechanism of HLJDD and HLJDD-NBA in relieving UC in mice from a holistic perspective.

Methods: The acute UC model of BABL/c mice was induced by 3.5% (w/v) dextran sodium sulfate drinking water. At the same time of modeling, HLJDD and HLJDD-NBA were given orally for treatment respectively. During the experiment, the clinical symptoms of mice were recorded and the physiological and biochemical indexes of mice were detected after the experiment. In addition, the plasma metabolites of mice in each group were detected and analyzed by ultra-high performance liquid chromatography quadrupole time of flight mass spectrometry and multivariate statistical analysis method. Then, the potential target metabolic pathway of drug intervention was screened through the enrichment analysis of differential metabolites. Finally, we use molecular simulation docking technology to further explore the molecular regulatory mechanism of HLJDD and HLJDD-NBA on potential target metabolic pathways.

Results: HLJDD and HLJDD-NBA intervention can significantly reduce the disease activity index of UC mice, inhibit colon length shortening and pathological damage, and relieve the abnormal changes of physiological and biochemical parameters of UC mice. Moreover, HLJDD and HLJDD-NBA can significantly inhibit the metabolic dysfunction of UC mice by reversing the abnormal changes of 24 metabolites in UC mice, and the arachidonic acid metabolic pathway and glycerophospholipid metabolic pathway are the target metabolic pathways regulated by them. Further literature review and molecular simulation docking analysis showed that HLJDD and HLJDD-NBA may inhibit the disorder of arachidonic acid metabolism pathway and glycerophospholipid metabolism pathway by inhibiting COX-2 protein expression and PLA2, 5-LOX activity.

Conclusions: Our experiments revealed that HLJDD and HLJDD-NBA can alleviate UC of mice by regulating arachidonic acid metabolism and glycerophospholipid metabolism, which points out the direction for further research and development of HLJDD as a new anti-ulcer drug.
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http://dx.doi.org/10.1016/j.jep.2020.112872DOI Listing
September 2020

Dual-Template Pore Engineering of Whey Powder-Derived Carbon as an Efficient Oxygen Reduction Reaction Electrocatalyst for Primary Zinc-Air Battery.

Chem Asian J 2020 Jun 14;15(12):1881-1889. Epub 2020 May 14.

School of Chemical and Biomolecular Engineering, The University of Sydney New South Wales, 2006, Darlington, Australia.

Cost-effective and high-performance electrocatalysts for oxygen reduction reactions (ORR) are needed for many energy storage and conversion devices. Here, we demonstrate that whey powder, a major by-product in the dairy industry, can be used as a sustainable precursor to produce heteroatom doped carbon electrocatalysts for ORR. Rich N and S compounds in whey powders can generate abundant catalytic active sites. However, these sites are not easily accessible by reactants of ORR. A dual-template method was used to create a hierarchically and interconnected porous structure with micropores created by ZnCl and large mesopores generated by fumed SiO particles. At the optimum mass ratio of whey power: ZnCl  : SiO at 1 : 3 : 0.8, the resulting carbon material has a large specific surface area close to 2000 m  g , containing 4.6 at.% of N with 39.7% as pyridinic N. This carbon material shows superior electrocatalytic activity for ORR, with an electron transfer number of 3.88 and a large kinetic limiting current density of 45.40 mA cm . They were employed as ORR catalysts to assemble primary zinc-air batteries, which deliver a power density of 84.1 mW cm and a specific capacity of 779.5 mAh g , outperforming batteries constructed using a commercial Pt/C catalyst. Our findings open new opportunities to use an abundant biomaterial, whey powder, to create high-value-added carbon electrocatalysts for emerging energy applications.
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http://dx.doi.org/10.1002/asia.202000399DOI Listing
June 2020

Electronic Modulation of Nickel Disulfide toward Efficient Water Electrolysis.

Small 2020 Apr 3;16(17):e1905885. Epub 2020 Apr 3.

Energy Research [email protected] ([email protected]), Interdisciplinary Graduate School, Nanyang Technological University, Singapore, 637553, Singapore.

Developing highly efficient earth-abundant nickel-based compounds is an important step to realize hydrogen generation from water. Herein, the electronic modulation of the semiconducting NiS by cation doping for advanced water electrolysis is reported. Both theoretical calculations and temperature-dependent resistivity measurements indicate the semiconductor-to-conductor transition of NiS after Cu incorporation. Further calculations also suggest the advantages of Cu dopant to cathodic water electrolysis by bringing Gibbs free energy of H adsorption at both Ni sites and S sites much closer to zero. It is noteworthy that water dissociation on Cu-doped NiS (Cu-NiS ) surface is even more favorable than those on NiS and Pt(111). Thus, the prepared Cu-NiS shows noticeably improved performance toward alkaline hydrogen and oxygen evolution reactions (HER and OER). Specifically, it requires merely 232 mV OER overpotential to drive 10 mA cm ; in parallel with Tafel slopes of 46 mV dec . Regarding HER, an onset overpotential of only 68 mV is achieved. When integrated as both electrodes for water electrolysis, Cu-NiS needs only 1.64 V to drive 10 mA cm , surpassing the state-of-the-art Ir/C-Pt/C couple (1.71 V). This work opens up an avenue to engineer low-cost and earth-abundant catalysts performing on par with the noble-metal-based one for water splitting.
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http://dx.doi.org/10.1002/smll.201905885DOI Listing
April 2020

Cardanol-derived cationic surfactants enabling the superior antibacterial activity of single-walled carbon nanotubes.

Nanotechnology 2020 Apr 27;31(26):265603. Epub 2020 Feb 27.

Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, People's Republic of China.

Single-walled carbon nanotubes (SWCNTs) are potential antibacterial material, and their antibacterial activity in aqueous solutions depends on efficient surfactants to create strong interactions between well-dispersed SWCNTs and bacterial cells. Here, we designed and synthesized a new family of cationic surfactants by introducing different positively charged hydrophilic heads, i.e. -(CH)N(CH)Br, -(CH)N(CH)Br and -(CH)NPyridineBr, to cardanol obtained from cashew nut shell liquid. These surfactants can efficiently disperse SWCNTs in aqueous solutions because benzene rings and olefin chains in cardanol enable their strong π-stacking on SWCNTs. A much higher fraction of SWCNTs can be dispersed individually compared to the commonly used surfactant, dodecylbenzene-sulfonate sodium (SDBS). SWCNTs dispersed in the cardanol-derived surfactants demonstrate significantly improved antibacterial activity. At the concentration of 0.5 wt%, their minimum inhibitory concentration is 0.33 and 0.02 μg ml against E. coli and S. aureus, respectively, which is only 0.8%-1.5% of that of SDBS-dispersed SWCNTs. The strong antibacterial activity can be attributed to both better dispersion of SWCNTs and positive charges introduced by hydrophilic heads, which are attracted to negatively charged bacterial cell surfaces. These cardanol-derived surfactants are promising as sustainable surfactants for enabling various SWCNT applications.
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http://dx.doi.org/10.1088/1361-6528/ab7aa4DOI Listing
April 2020

A Flexible Rechargeable Zinc-Air Battery with Excellent Low-Temperature Adaptability.

Angew Chem Int Ed Engl 2020 Mar 4;59(12):4793-4799. Epub 2020 Feb 4.

School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, New South Wales, 2006, Australia.

Flexible zinc-air batteries (ZAB) are a promising battery candidate for emerging flexible electronic devices, but the catalysis-based working principle and unique semi-opened structure pose a severe challenge to their overall performance at cold temperature. Herein, we report the first flexible rechargeable ZAB with excellent low-temperature adaptability, based on the innovation of an efficient electrocatalyst to offset the electrochemical performance shrinkage caused by decreased temperature and a highly conductive hydrogel with a polarized terminal group to render the anti-freezing property. The fabricated ZABs show excellent electrochemical performances that outperform those of many aqueous ZABs at room temperature. They also deliver a high capacity of 691 mAh g and an energy density of 798 Wh kg at -20 °C (92.7 % and 87.2 % retention of the room temperature counterparts, respectively), together with excellent flexibility and reverting capability.
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http://dx.doi.org/10.1002/anie.201915836DOI Listing
March 2020

Huang-Lian-Jie-Du Decoction Ameliorates Acute Ulcerative Colitis in Mice Regulating NF-κB and Nrf2 Signaling Pathways and Enhancing Intestinal Barrier Function.

Front Pharmacol 2019 21;10:1354. Epub 2019 Nov 21.

Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.

Evidence shows that intestinal inflammation, oxidative stress, and injury of mucosal barrier are closely related to the pathogenesis of ulcerative colitis (UC). Huang-lian-Jie-du Decoction (HLJDD) is a well-known prescription of traditional Chinese medicine with anti-inflammatory and antioxidative activities, which may be used to treat UC. However, its therapeutic effect and mechanism are still unclear. In this study, the UC model of BABL/c mice were established by DSS [3.5% (w/v)], and HLJDD was given orally for treatment at the same time. During the experiment, the clinical symptoms of mice were scored by disease activity index (DAI). Besides, the effects of HLJDD on immune function, oxidative stress, colon NF-κB and Nrf2 signaling pathway, and intestinal mucosal barrier function in UC mice were also investigated. The results showed that HLJDD could alleviate body weight loss and DAI score of UC mice, inhibit colonic shortening and relieve colonic pathological damage, and reduce plasma and colon MPO levels. In addition, HLJDD treatment significantly up-regulated plasma IL-10, down-regulated TNF-α and IL-1β levels, and inhibited the expression of NF-κB p65, p-IκKα/β, and p-IκBα proteins in the colon. Moreover, NO and MDA levels in colon tissues were significantly reduced after HLJDD treatment, while GSH, SOD levels and Nrf2, Keap1 protein expression levels were remarkably elevated. Additionally, HLJDD also protected intestinal mucosa by increasing the secretion of mucin and the expression of ZO-1 and occludin in colonic mucosa. These results indicate that HLJDD could effectively alleviate DSS-induced mice UC by suppressing NF-κB signaling pathway, activating Nrf2 signaling pathway, and enhancing intestinal barrier function.
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http://dx.doi.org/10.3389/fphar.2019.01354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6900672PMC
November 2019

Therapeutic effect of n-butanol fraction of Huang-lian-Jie-du Decoction on ulcerative colitis and its regulation on intestinal flora in colitis mice.

Biomed Pharmacother 2020 Jan 22;121:109638. Epub 2019 Nov 22.

Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China. Electronic address:

Huang-lian-Jie-du Decoction (HLJDD) is a classical prescription for clearing away heat and detoxification. In order to screen the effective fraction of HLJDD in the treatment of ulcerative colitis (UC) in mice and explore its effects on intestinal flora in UC mice, we prepared different polar fractions of HLJDD by system solvent extraction method. Subsequently, the contents of 13 active compounds in different polar fractions of HLJDD were determined by HPLC. Further, the UC model induced by dextran sodium sulfate was used to evaluate the therapeutic effects of different polar fractions of HLJDD. Finally, cecal contents were used for sequencing and analysis of bacterial 16S rRNA genes. The results showed that the yield of HLJDD-n-butanol (HLJDD-NBA) fraction was the highest, and the content or proportion of 13 active compounds in HLJDD-NBA fraction were the most similar to HLJDD. In addition, in vivo pharmacodynamic experiments showed that HLJDD-NBA intervention not only significantly alleviated the clinical symptoms of UC mice and ameliorated the pathological damage of colon tissue, but also showed significant anti-inflammatory and antioxidative effects (p < 0.05), which were comparable to HLJDD (p > 0.05). Moreover, both HLDD and HLJDD-NBA treatments can restore the intestinal flora homeostasis of UC mice by inhibiting the growth of intestinal pathogens and preventing the decrease of beneficial bacteria. Meanwhile, they can also significantly correct the dysfunction of intestinal flora in UC mice. In conclusion, we proved that HLJDD-NBA fraction is an effective fraction of HLJDD in treating UC in mice, and it can maintain the intestinal flora homeostasis of UC mice, which increases our understanding of the mechanism of HLJDD in treating UC and lays a foundation for the development of new anti-ulcer drugs.
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http://dx.doi.org/10.1016/j.biopha.2019.109638DOI Listing
January 2020

Flexible Zinc-Ion Hybrid Fiber Capacitors with Ultrahigh Energy Density and Long Cycling Life for Wearable Electronics.

Small 2019 11 14;15(47):e1903817. Epub 2019 Oct 14.

School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, NSW, 2006, Australia.

Emerging wearable electronics require flexible energy storage devices with high volumetric energy and power densities. Fiber-shaped capacitors (FCs) offer high power densities and excellent flexibility but low energy densities. Zn-ion capacitors have high energy density and other advantages, such as low cost, nontoxicity, reversible Faradaic reaction, and broad operating voltage windows. However, Zn-ion capacitors have not been applied in wearable electronics due to the use of liquid electrolytes. Here, the first quasisolid-state Zn-ion hybrid FC (ZnFC) based on three rationally designed components is demonstrated. First, hydrothermally assembled high surface area and conductive reduced graphene oxide/carbon nanotube composite fibers serve as capacitor-type positive electrodes. Second, graphite fibers coated with a uniform Zn layer work as battery-type negative electrodes. Third, a new neutral ZnSO -filled polyacrylic acid hydrogel act as the quasisolid-state electrolyte, which offers high ionic conductivity and excellent stretchability. The assembled ZnFC delivers a high energy density of 48.5 mWh cm at a power density of 179.9 mW cm . Further, Zn dendrite formation that commonly happens under high current density is efficiently suppressed on the fiber electrode, leading to superior cycling stability. Multiple ZnFCs are integrated as flexible energy storage units to power wearable devices under different deformation conditions.
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http://dx.doi.org/10.1002/smll.201903817DOI Listing
November 2019

Prevalence and Characteristics of Quinolone Resistance in Isolated from Retail Foods in Lanzhou, China.

J Food Prot 2019 Sep;82(9):1591-1597

Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.

The aim of this study was to determinate the prevalence of in retail foods and its resistance to quinolones in retail foods in Lanzhou, People's Republic of China. In this work, 2,182 food samples, collected from March 2015 to December 2018, were analyzed to detect and then analyzed for serotype distribution, quinolone resistance, and quinolone-resistant gene detection. The findings demonstrate that the overall prevalence of in these food categories was low. A total of 41 (1.9%) of 2,182 food samples were found to be positive for . Ten distinct serovars were identified, and Derby, Anatum, and Enteritidis were the most prevalent serovars. According to the broth microdilution test, the resistance percentages were 90.2% to nalidixic acid, 39.0% to enrofloxacin, 41.5% to ciprofloxacin, 29.3% to ofloxacin, and 26.8% to levofloxacin. Among the quinolone-resistant isolates, 12 strains had a single mutation in at codon 83 (Ser→Phe) or codon 87 (Asp→Asn or Asp→Gly). Five isolates had one mutation (Ser80→Arg) and one or two hot spot mutations. genes were found in seven isolates (five and two ), and the gene in seven isolates. Two isolates carry both and genes. Based on these results, a low prevalence of contamination in retail foods was found, but it might play a potential risk factor in the spread of quinolone-resistant strains in the Lanzhou region.
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http://dx.doi.org/10.4315/0362-028X.JFP-19-001DOI Listing
September 2019

Nanocarbon materials in water disinfection: state-of-the-art and future directions.

Nanoscale 2019 May 13;11(20):9819-9839. Epub 2019 May 13.

The University of Sydney, School of Chemical and Biomolecular Engineering, NSW, 2006, Australia.

Water disinfection practices are critical for supplying safe drinking water. Existing water disinfection methods come with various drawbacks, calling for alternative or complementary solutions. Nanocarbon materials (NCMs) offer unique advantages for water disinfection owing to their high antimicrobial activity, often low environmental/human toxicity, and tunable physicochemical properties. Nevertheless, it is a challenge to assess the research progress made so far due to the structure and property diversity in NCMs as well as their different targeted applications. Because of these, here we provide a broad outline of this emerging field in three parts. First, we introduce the antimicrobial activities of the different types of NCMs, including fullerenes, nanodiamonds, carbon (nano)dots, carbon nanotubes, and graphene-family materials. Next, we discuss the current status in applying these NCMs for different water disinfection problems, especially as hydrogel filters, filtration membranes, recyclable aggregates, and electrochemical devices. We also introduce the use of NCMs in photocatalysts for photocatalytic water disinfection. Lastly, we put forward the key hurdles of the field that hamper the realization of the practical applications and propose possible directions for future investigations to address those. We hope that this minireview will encourage researchers to tackle these challenges and innovate NCM-based water disinfection platforms in the near future.
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http://dx.doi.org/10.1039/c9nr02007aDOI Listing
May 2019

Big to Small: Ultrafine Mo C Particles Derived from Giant Polyoxomolybdate Clusters for Hydrogen Evolution Reaction.

Small 2019 Mar 8;15(11):e1900358. Epub 2019 Feb 8.

School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, New South Wales, 2006, Australia.

Due to its electronic structure, similar to platinum, molybdenum carbides (Mo C) hold great promise as a cost-effective catalyst platform. However, the realization of high-performance Mo C catalysts is still limited because controlling their particle size and catalytic activity is challenging with current synthesis methods. Here, the synthesis of ultrafine β-Mo C nanoparticles with narrow size distribution (2.5 ± 0.7 nm) and high mass loading (up to 27.5 wt%) on graphene substrate using a giant Mo-based polyoxomolybdate cluster, Mo ((NH ) [Mo O (CH COO) (H O) ]·10CH COONH ·300H O) is demonstrated. Moreover, a nitrogen-containing polymeric binder (polyethyleneimine) is used to create MoN bonds between Mo C nanoparticles and nitrogen-doped graphene layers, which significantly enhance the catalytic activity of Mo C for the hydrogen evolution reaction, as is revealed by X-ray photoelectron spectroscopy and density functional theory calculations. The optimal Mo C catalyst shows a large exchange current density of 1.19 mA cm , a high turnover frequency of 0.70 s as well as excellent durability. The demonstrated new strategy opens up the possibility of developing practical platinum substitutes based on Mo C for various catalytic applications.
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http://dx.doi.org/10.1002/smll.201900358DOI Listing
March 2019

Metabolomics study on promoting blood circulation and ameliorating blood stasis: Investigating the mechanism of Angelica sinensis and its processed products.

Biomed Chromatogr 2019 Apr 9;33(4):e4457. Epub 2019 Jan 9.

Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, China.

Angelica sinensis (Danggui, DG) parched with alcohol (Jiu Danggui, JDG) and charred DG are the main processed products of DG, which are used to treat blood stasis syndrome (BSS). However, their therapeutic effect and mechanisms are still unclear. Based on an acute rat BSS model, the intervention effects of DG and its processed products (DGPPs) were evaluated by the hemorheology and coagulation function parameters. Meanwhile, plasma and urine metabolites were detected and analyzed by liquid chromatography coupled to quadrupole time-of-flight mass spectrometry and multivariate statistical analysis method. The results of hemorheology, coagulation function parameters and metabolomics all showed that the BSS model was successfully established, DGPPs intervention could significantly relieve rats BSS and the therapeutic effect of JDG was best. Moreover, 23 differential metabolites (14 in plasma and nine in urine) were identified that were closely related to the BSS, involving seven potential target metabolic pathways. DGPP intervention showed different degrees of reverse effect on these metabolites. JDG was the most effective owing to extensive regulation effect on differential metabolites. This study provides a reference for understanding the pathological mechanism of BSS and the mechanism of DGPPs, which lays a theoretical foundation for the rational use of DGPPs in clinical practice.
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http://dx.doi.org/10.1002/bmc.4457DOI Listing
April 2019

Urinary metabolomics study the mechanism of Taohong Siwu Decoction intervention in acute blood stasis model rats based on liquid chromatography coupled to quadrupole time-of-flight mass spectrometry.

J Chromatogr B Analyt Technol Biomed Life Sci 2018 Feb 2;1074-1075:51-60. Epub 2018 Jan 2.

Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address:

Taohong Siwu Decoction (TSD) is a classic prescription in traditional Chinese medicine and is widely used to promote blood circulation to remove blood stasis. However, the effect mechanisms are not yet well understood. Here, a urinary metabolomic approach based on liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (LC/Q-TOF-MS) was conducted to explore the changes in the endogenous metabolites and to assess the integral efficacy of TSD on acute blood stasis model rats. Then, parameters for hemorheology and coagulation functions were detected. Principal component analysis (PCA) and orthogonal partial least squares discriminate analysis (OPLS-DA) was used to investigate the global metabolite alterations and to evaluate the preventive effects of TSD in rats. Potential metabolite markers were found using OPLS-DA and t-test. Furthermore, metabolic pathway analysis was performed to construct metabolic networks. The results showed that TSD could significantly decrease whole blood viscosity and plasma viscosity. It also significantly prolonged partial thromboplastin time (APPT) and prothrombin time (PT), increased thrombin time (TT) and lowered fibrinogen content (FIB). Moreover, 24 potential metabolite markers of acute blood stasis were screened, and the levels were all reversed to different degrees after TSD administration. In metabolic networks, amino acid metabolism (arginine and proline metabolism; histidine metabolism; alanine, aspartate, and glutamate metabolism; phenylalanine, tyrosine, and tryptophan biosynthesis; phenylalanine metabolism) and lipid metabolism (glycerophospholipid metabolism; linoleic acid metabolism; alpha-linolenic acid metabolism) were closely related with the intervention mechanism of TSD on acute blood stasis. The urinary metabolomic approach can be applied to clarify the mechanism of TSD in promoting blood circulation to remove acute blood stasis and to provide the theoretical basis for further research on the therapeutic mechanism of TSD in clinical practice.
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http://dx.doi.org/10.1016/j.jchromb.2017.12.035DOI Listing
February 2018

A novel approach using metabolomics coupled with hematological and biochemical parameters to explain the enriching-blood effect and mechanism of unprocessed Angelica sinensis and its 4 kinds of processed products.

J Ethnopharmacol 2018 Jan 25;211:101-116. Epub 2017 Sep 25.

Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.

Ethnopharmacological Relevance: Angelica sinensis (AS), root of Angelica sinensis (Oliv.) Diels, an important kind of Chinese traditional herbal medicine, has been used for women to enrich the blood for thousands of years. It is mainly distributed in Gansu province of China. According to Traditional Chinese medicine usage, unprocessed AS (UAS) and its 4 kinds of processed products (ASs) are all used to treat different diseases or syndromes. The difference among the enriching-blood effects of ASs is unclear. And their exact mechanisms of enriching the blood are not fully understood.

Aim Of The Study: In this study, our aim is to compare the enriching-blood effect and explain the related mechanism of ASs, to lay the foundation for the blood deficiency diagnosis and the rational use of ASs in the clinic.

Materials And Methods: ASs were used to intervene the blood deficiency syndrome model mice induced by acetyl phenylhydrazine (APH) and cyclophosphamide (CTX). A novel approach using metabolomics coupled with hematological and biochemical parameters to explain the enriching-blood effect and mechanism of ASs was established. The blood routine examination, ATPase, glucose-6-phosphate dehydrogenase, methemoglobin, glutathion peroxidase, glutathione reductase, and erythropoietin were measured. Two biofluids (plasma and urine) obtained from mice were analyzed with GC-MS. Distinct changes in metabolite patterns of the two biofluids after mice were induced by APH and CTX, and mice were intervened with ASs were analyzed using partial least squares-discriminant analysis. Potential biomarkers were found using a novel method including variable importance in the projection (VIP) >1.0, volcano plot analysis, and significance analysis of microarray.

Results: The results of hematological, biochemical parameters and the integrated metabolomics all showed the blood deficiency syndrome model was built successfully, ASs exhibited different degree of enriching-blood effect, and AS pached with alcohol (AAS) exhibited the best enriching-blood effect. 16 metabolites in the plasma and 8 metabolites in the urine were considered as the potential biomarkers. These metabolites were involved in 7 metabolic pathways which were concerned with the different enriching-blood effect mechanisms of ASs. The correlation analysis results confirmed L-Valine (plasma), Linoleic acid (urine), L-Aspartic acid (urine) and Cholesterol (urine) were strong positive or negative associated with biochemical indicators.

Conclusions: The enriching-blood effects of ASs are different. The pathological mechanisms of blood deficiency syndrome and the enriching-blood effect mechanism of ASs are involved in 7 metabolic pathways. L-Valine (plasma), Linoleic acid (urine), L-Aspartic acid (urine), Cholesterol (urine) are four important biomarkers being related to the enriching-blood effect of ASs. The combination of VIP, volcano plot analysis and significance analysis of microarray is suitable for screening biomarkers in metabolomics study. They can lay the foundation for clinical practice.
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http://dx.doi.org/10.1016/j.jep.2017.09.028DOI Listing
January 2018

Molecular Insights into the Transformation of Dissolved Organic Matter in Landfill Leachate Concentrate during Biodegradation and Coagulation Processes Using ESI FT-ICR MS.

Environ Sci Technol 2017 Jul 30;51(14):8110-8118. Epub 2017 Jun 30.

Department of Environmental Engineering, Peking University , Beijing 100871, China.

Landfill leachate concentrate is a type of refractory organic wastewater with high environmental risk. Identification of refractory components and insights into the molecular transformations of the organics are essential for the development of efficient treatment process. In this report, molecular compositions of dissolved organic matter (DOM) in leachate concentrate, as well as changes after anaerobic/aerobic biodegradation and coagulation with salts, were characterized using electrospray ionization (ESI) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). DOM in leachate concentrate were more saturated and less oxidized with more nitrogen and sulfur-containing substances (accounting for 50.0%), comparing with natural organic matter in Suwannee River. Selectivity for different classes of organics during biodegradation and coagulation processes was observed. Substances with low oxidation degree (O/C < 0.3) were more reactive during biodegradation process, leading to the formation of highly oxidized molecules (O/C > 0.5). Unsaturated (H/C < 1.0) and oxidized (O/C > 0.4) substances containing carboxyl groups were preferentially removed after coagulation with Al or Fe sulfate. The complementary functions of biodegradation and coagulation in the treatment of DOM in leachate concentrate were verified at the molecular level. Lignin-derived compounds and sulfur-containing substances in leachate concentrate were resistant to biodegradation and coagulation treatments. To treat leachate concentrate more effectively, processes aimed at removal of such DOM should be developed.
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http://dx.doi.org/10.1021/acs.est.7b02194DOI Listing
July 2017

Optimal Systolic Blood Pressure Levels for Primary Prevention of Stroke in General Hypertensive Adults: Findings From the CSPPT (China Stroke Primary Prevention Trial).

Hypertension 2017 04 27;69(4):697-704. Epub 2017 Feb 27.

From the Department of Cardiology, Peking University First Hospital, Beijing, China (F.F., J.L., Y.Z., Y.H.); Department of Neurology, Xijing Hospital, the Fourth Military Medical University, Xi'an, China (Z.Y., G.Z.); National Clinical Research Center for Kidney Disease; State Key Laboratory for Organ Failure Research; Renal Division, Nanfang Hospital, Southern Medical University, Guangzhou, China (X.Q., Y.L., F.H., X.X.); Institute for Biomedicine, Anhui Medical University, Hefei, China (X.Q., T.Y.); Shanghai Institute of Cardiovascular Diseases, Department of Cardiology, Zhongshan Hospital (M.J., J.G.), and Institutes of Biomedical Sciences (M.J., J.G.), Fudan University, Shanghai, China; Department of Cardiology, Beijing Chaoyang Hospital, Capital Medical University, China (M.Z., X.Y.); Department of Cardiology, Second Affiliated Hospital, Nanchang University, China (H.B., X.C.); Department of Epidemiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China (D.G.); Department of Epidemiology, Capital Medical University Beijing Anzhen Hospital-Beijing Institute of Heart, Lung & Blood Vessel Diseases, China (D.Z.); Centre for Epidemiological Studies and Clinical Trials, Ruijin Hospital, The Shanghai Institute of Hypertension, Shanghai Jiaotong University School of Medicine, China (J.W.); Department of Cardiology, Peking University People's Hospital, Beijing, China (N.S.); Department of Cardiology, Chinese People's Liberation Army General Hospital, Beijing, China (Y.C.); Centers for Metabolic Disease Research, Temple University School of Medicine, Philadelphia, PA (H.W.); Department of Population, Family and Reproductive Health, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD (Xiaobin Wang); Department of Cardiovascular, Neural and Metabolic Sciences, San Luca Hospital, Istituto Auxologico Italiano, Milan, Italy (G.P.); Department of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy (G.P.); and Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China (Xian Wang).

We aimed to investigate the relationship of time-averaged on-treatment systolic blood pressure (SBP) with the risk of first stroke in the CSPPT (China Stroke Primary Prevention Trial). A post hoc analysis was conducted using data from 17 720 hypertensive adults without cardiovascular disease, diabetes mellitus, and renal function decline from the CSPPT, a randomized double-blind controlled trial. The primary outcome was first stroke. Over a median follow-up duration of 4.5 years, the association between averaged on-treatment SBP and risk for first stoke followed a U-shape curve, with increased risk above and below the reference range of 120 to 130 mm Hg. Compared with participants with time-averaged on-treatment SBP at 120 to 130 mm Hg (mean, 126.2 mm Hg), the risk of first stroke was not only increased in participants with SBP at 130 to 135 mm Hg (mean, 132.6 mm Hg; 1.5% versus 0.8%; hazard ratio, 1.63; 95% confidence interval, 1.01-2.63) or 135 to 140 mm Hg (mean, 137.5 mm Hg; 1.9% versus 0.8%; hazard ratio, 1.85; 95% confidence interval, 1.17-2.93), but also increased in participants with SBP <120 mm Hg (mean, 116.7 mm Hg; 3.1% versus 0.8%; hazard ratio, 4.37; 95% confidence interval, 2.10-9.07). Similar results were found in various subgroups stratified by age, sex, and treatment group. Furthermore, lower diastolic blood pressure was associated with lower risk of stroke, with a plateau at a time-average on-treatment diastolic blood pressure <80 mm Hg. In conclusion, among adults with hypertension and without a history of stroke or myocardial infarction, diabetes mellitus, or renal function decline, a lower SBP goal of 120 to 130 mm Hg, as compared with a target SBP of 130 to 140 mm Hg or <120 mm Hg, resulted in the lowest risk of first stroke.
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.116.08499DOI Listing
April 2017

Intravenous thrombolysis guided by a telemedicine consultation system for acute ischaemic stroke patients in China: the protocol of a multicentre historically controlled study.

BMJ Open 2015 May 15;5(5):e006704. Epub 2015 May 15.

Department of Neurology, Xijing Hospital, The Fourth Military Medical University, Xìan, China.

Introduction: The rate of intravenous thrombolysis with tissue-type plasminogen activator or urokinase for stroke patients is extremely low in China. It has been demonstrated that a telestroke service may help to increase the rate of intravenous thrombolysis and improve stroke care quality in local hospitals. The aim of this study, also called the Acute Stroke Advancing Program, is to evaluate the effectiveness and safety of decision-making concerning intravenous thrombolysis via a telemedicine consultation system for acute ischaemic stroke patients in China.

Methods And Analysis: This is a multicentre historically controlled study with a planned enrolment of 300 participants in each of two groups. The telestroke network consists of one hub hospital and 14 spoke hospitals in underserved regions of China. The usual stroke care quality in the spoke hospitals without guidance from the hub hospital will be used as the historical control. The telemedicine consultation system is an interactive, two-way, wireless, audiovisual system accessed on portable devices. The primary outcome is the percentage of patients treated with intravenous thrombolysis within 4.5 h of stroke onset.

Ethics And Dissemination: The project has been approved by the Institutional Review Board of Xijing Hospital. The results will be published in scientific journals and presented to local government and relevant institutes.

Trial Registration Number: NCT02088346 (12 March 2014).
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http://dx.doi.org/10.1136/bmjopen-2014-006704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442242PMC
May 2015
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