Publications by authors named "Ziwei Zhang"

221 Publications

Identification of CCT3 as a prognostic factor and correlates with cell survival and invasion of head and neck squamous-cell carcinoma.

Biosci Rep 2021 Sep 10. Epub 2021 Sep 10.

The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Recurrent locally advanced or metastatic head and neck squamous cell carcinoma (HNSCC) is associated with dismal prognosis because of its highly invasive behavior and resistance to conventional intensive chemotherapy. The identification of effective markers for early diagnosis and prognosis is important for reducing mortality and ensuring that therapy for HNSCC is effective. Chaperonin-containing TCP-1 3 (CCT3) folds cancer-related proteins to control carcinogenesis. The prognostic value and growth association of CCT3 and HNSCC remains unknown.

Methods: The GEO, Oncomine and UALCAN databases were used to examine CCT3 expression in HNSCC. A few clinical HNSCC samples with normal tissues were used to detect CCT3 expression by using immunohistochemistry method. The TCGA-HNSC dataset was used to evaluate the association between expression of CCT3 and prognosis. The molecular mechanism was investigated with gene set enrichment analysis (GSEA). CCK-8 and wound healing assays were used to detect cell growth and invasion of HNSCC, respectively.

Results: CCT3 expression was significantly upregulated in HNSCC in both mRNA and protein levels. In addition, upregulated CCT3 expression was associated with various clinicopathological parameters. High expression of CCT3 was significantly correlated with inferior survival of HNSCC patients. Knockdown of CCT3 significantly inhibited cell growth and invasion of HNSCC cell lines. GSEA analysis indicated that CCT3 was closely correlated with tumor-related signaling pathways and HNSCC cell survival.

Conclusion: Our findings suggest that CCT3 is a biomarker of poor prognosis and related to the process of HNSCC.
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http://dx.doi.org/10.1042/BSR20211137DOI Listing
September 2021

Childhood adversity subtypes and violence victimization and perpetration among early adolescents in Shanghai, China.

BMC Pediatr 2021 09 3;21(1):381. Epub 2021 Sep 3.

NHC Key Lab of Reproduction Regulation (Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan University, NO.779 Old Humin Road, Xuhui District, 200237, Shanghai, P.R. China.

Background: This cross-sectional study aimed to identify adverse childhood experience (ACE) subtypes using variable- and person-centered approaches and examine the possible sex-differentiated associations with violence involvement as victim, perpetrator, and victim-perpetrator.

Methods: Adolescents aged 10-14 years in three junior high schools in Shanghai, China, were selected using a cluster sampling method in November and December 2017. Participants were surveyed anonymously using a computer-assisted self-interview approach via tablets. Thirteen items modified from the CDC-Kaiser ACE study were used to measure the ACEs. Results show subtypes as neglect, abuse, and household dysfunction by developing cumulative index score from the variable perspective and subgroups identified through the latent class analysis (LCA) from the person perspective. Logistic regression analyses were used to test the association between each ACE subtype and violence victimization and perpetration after adjusting for some demographic characteristics.

Results: A total of 1,700 participants were included in the final analysis. Approximately 1,322 (77.76 %) participants reported experiencing at least one ACE. The prevalence of neglect, abuse, and household dysfunction was 64.12 % (n = 1090), 61.29 % (n = 1042), and 18.24 % (n = 310), respectively. Three classes were identified through the LCA: low exposure to all ACEs (n = 854, 50.23 %), high exposure to emotional and physical abuse and neglect (n = 715, 42.06 %), and high exposure to all ACEs (n = 131,7.71 %). After controlling the covariates, experiencing abuse, neglect, and household dysfunction was significantly related to violence victimization (adjusted odds ratio [aOR] = 3.19, 3.29, 2.37, P < 0.001) and victim-perpetrator (aOR = 3.48, 4.41, 5.16, P < 0.001). Adolescent violence perpetration was only found to be associated with being neglected (aOR = 2.37, P = 0.003) and suffering household dysfunction (aOR = 3.25, P < 0.001). LCA revealed the cumulative effects of ACEs on adolescent violence victimization and perpetration. Sex-stratified analysis indicate that girls were more vulnerable to the negative effects of ACEs, with a higher risk of perpetration among girls exposed to distinctive subtypes or multiple ACEs.

Conclusions: ACEs were ubiquitous and significantly associated with an elevated risk of violence victimization and perpetration during early adolescence. Future research should examine whether these associations persist over time and the intermediating mechanism from the perspectives of individual neurodevelopment, cognition and resilience ability, and social support.
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http://dx.doi.org/10.1186/s12887-021-02853-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414739PMC
September 2021

On the Correlation among Edge, Pose and Parsing.

IEEE Trans Pattern Anal Mach Intell 2021 Sep 1;PP. Epub 2021 Sep 1.

Semantic parsing, edge detection and pose estimation of human are three closely-related tasks. They present human characteristics from three complementary aspects. Compared to learning them individually, solving these tasks jointly can explore the interaction of their contextual cues. However, prior works usually study the fusion of two of them, e.g., parsing and pose, parsing and edge. In this paper, we explore how pixel-level semantics, human boundaries and joint locations can be effectively learned in a unified model. Specifically, we propose an end-to-end trainable Human Task Correlation Machine (HTCorrM) to implement the three tasks. It is asymmetric in that it supports a main task using the other two as auxiliary tasks. We also introduce a Heterogeneous Non-Local module (HNL) to discover the correlations of the three heterogeneous domains. HNL fully explores the global dependency among tasks between any two positions in the feature map. Experimental results on human parsing, pose estimation and body edge detection demonstrate that HTCorrM achieves competitive performance. We show that when designated as the main task, the accuracy of each of the three tasks is improved. Importantly, comparative studies confirm the advantages of our proposed feature correlation strategy over the traditional feature concatenation or post processing.
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http://dx.doi.org/10.1109/TPAMI.2021.3108771DOI Listing
September 2021

Cadmium exposure promotes activation of cerebrum and cerebellum ferroptosis and necrosis in swine.

Ecotoxicol Environ Saf 2021 Aug 14;224:112650. Epub 2021 Aug 14.

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address:

Cadmium(Cd) is a toxic and carcinogenic heavy metal pollutant leading to serious damage in various organs. Ferroptosis and necrosis as inflammation-related cell death are involved in several diseases of nervous system. In the present study, 10 weaning piglets with similar weight for 6 weeks were randomly divided into two groups. The daily grain containing 0 mg and 20 mg/kg of Cd chloride was fed in 20-26 ℃ environment, animals were sacrificed to collect cerebrum and cerebellum tissues after 40 days. Morphology and ultrastructure results were observed using HE and TEM. Moreover, molecular biological technologies western blot and qRT-PCR were used to detect the expression abundance of genes. Cerebrum and cerebellum injury was observed in Cd-exposed group, antioxidant capacity decreased significantly and oxidative stress increased; immunofluorescence, real-time quantification, and western blot results showed decreased necrosis genes and increased ferroptosis pathway genes abundance in cerebrum, whereas the results were reversed in cerebellum. These results indicated that Cd exposure can activated necrosis and ferroptosis pathways by increased oxidative stress, further resulting in cerebrum and cerebellum damage in pigs. These findings may provide a theoretical basis for early monitoring of Cd exposure in environment.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112650DOI Listing
August 2021

Cadmium exposure triggers oxidative stress, necroptosis, Th1/Th2 imbalance and promotes inflammation through the TNF-α/NF-κB pathway in swine small intestine.

J Hazard Mater 2021 Jul 20;421:126704. Epub 2021 Jul 20.

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China. Electronic address:

Cadmium (Cd) is a toxic environmental pollutant and induces toxic effects to organism. Nevertheless, the mechanism of Cd-induced toxicity in swine remains obscure. To explore this, 10 healthy 6-week-old weaned swine were placed into two groups stochastically, the Cd group was treated with a commercial diet containing 20 mg/kg Cd for 40 days. The results of histopathological and ultrastructural observations showed typical necrosis features and inflammatory cell infiltration in Cd group. Excessive Cd suppressed T-AOC and SOD activities, increased MDA content and ROS levels. Cd diet elevated the expression of RIPK1, RIPK3, and MLKL to activate the RIPK3-dependent necroptosis pathway. Results of Th1 and Th2 cytokines indicated that the levels of IL-4, IL-6 and IL10 was increased, while the level of IFN-γ was decreased, illustrating Th1/Th2 immune imbalance leads to aggravate inflammatory responses. Cd activated the TNF-α/NF-κB pathway and induced inflammatory responses via increasing the expression of HO-1, IL-1β, iNOS, COX2. Heat shock proteins were notably elevated in response to inflammatory reactions. And these effects were inhibited by necrostatin-1 (Nec-1) and N-acetyl-cysteine (NAC). Altogether, these data demonstrated that Cd induced necroptosis and inflammation to aggravate small intestine injury in swine by increasing the excessive accumulation of ROS and imbalanced Th1/Th2, respectively.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126704DOI Listing
July 2021

Ergosterol peroxide: an effect-directed detecting method from and evaluation of anticancer activity.

Nat Prod Res 2021 Jul 13:1-6. Epub 2021 Jul 13.

Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou, P.R. China.

Ergosterol peroxide (EP) in possesses antioxidant and anticancer properties, yet few studies have been focused on its mechanisms and directed detecting. By practicing HPTLC-DPPH coupled with UHPLC-ESI-TOF MS, EP was located and the cytotoxic activity of EP was performed by MTT method. The apoptosis studies were conducted on SGC-7901cells by AO/EB and Annex V/PI staining method, PI flow cytometric assay, reactive oxygen species detection and mitochondrial membrane potential assay. An effect-directed detecting method of HPTLC-DPPH/UHPLC/ESI-TOF-MS was developed for EP rapidly and precisely, providing an option for identifying oxidative compounds. EP exhibits high cytotoxic activity against SGC-7901 gastric cancer cells and the morphological apoptosis suggested that EP induced apoptosis and cell cycle arrest in G0/G1 phase. It could enhance the ROS level and cause a decrease in the mitochondrial membrane potential. Its antiproliferative mechanism is G0/G1 phase arrest and might be traced through ROS-mediated mitochondrial dysfunction pathways.
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http://dx.doi.org/10.1080/14786419.2021.1949593DOI Listing
July 2021

Silver-enhanced fluorescence of bimetallic Au/Ag nanoclusters as ultrasensitive sensing probe for the detection of folic acid.

Talanta 2021 Oct 17;233:122469. Epub 2021 May 17.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, Jilin, China; University of Science and Technology of China, Hefei, 230026, China. Electronic address:

Folic acid (FA) is the natural form of water-soluble vitamins widely found in most plants and animal products and its deficiency leads to several human body abnormalities. The advancements of metal nanoclusters are highly increasing due to their molecule-like optical properties and attractive applications. Because of increasingly demand of noble metal nanoclusters as sensing templates, different synthesis methods have been developed for facile synthesis of noble metal nanoclusters. Herein, red-emitting fluorescent bovine serum albumin (BSA)-capped Au-Ag bimetallic NCs are facilely synthesized through green one-pot synthetic approach. The effect of silver on the fluorescence properties of Au NCs was investigated and it was found that introduction of silver can enhance the fluorescence intensity. The fluorescence intensity of the as-prepared Au-Ag nanoclusters gets quenched in the presence of folic acid in an aqueous medium and it was used as ultrasensitive sensing probe for FA detection. The developed Au-Ag NCs-based sensing probe shows linear response in the wide range of 0-100 μM and the detection limit is as low as 0.47 nM. Its applicability has also been confirmed successfully in real human serum, urine and FA tablet samples. Due to the high stability, sensitivity and selectivity, the developed bimetallic cluster sensing system is highly promising to be applied in the pharmaceutical and clinical laboratories.
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http://dx.doi.org/10.1016/j.talanta.2021.122469DOI Listing
October 2021

Application of disk tube reverse osmosis in wastewater treatment: A review.

Sci Total Environ 2021 Oct 5;792:148291. Epub 2021 Jun 5.

Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, China; Beijing Laboratory for Environmental Frontier Technologies, Beijing 100084, China; Shenzhen Environmental Science and New Energy Technology Engineering Laboratory, Tsinghua-Berkeley Shenzhen Institute, Shenzhen 518055, China.

Disk tube reverse osmosis (DTRO), a modified module RO, has received growing attention in wastewater treatment. However, there is no comprehensive review of DTRO applications for wastewater treatment. In this study, China was found to be a major contributor to DTRO investigations. Specifically, 46 full- and 17 pilot-scale DTRO applications in China from the literature are summarized. The cumulative reported DTRO application scale in wastewater treatment amounted to ~16,500 m/d by 2020 in China. Leachate and flue gas desulfurization (FGD) wastewater had the highest reported frequencies. Two-stage DTRO and "biological treatment + DTRO/two-stage DTRO" were the most reported processes in leachate treatment. Chemical oxygen demand (COD) and NH-N were removed at 99.00-99.95% and 98.00-99.98%, respectively, in full scales in leachate treatment. DTRO was primarily utilized in the concentration unit in hypersaline wastewater treatment (e.g., FGD wastewater). Total dissolved solids (TDS) were removed at 94.69-96.87% and 85.95-96.5% in the full- and pilot-scales, respectively. The overall operating costs were 17.50-60.61 CNY/m and 0.69-8.75 CNY/kgCOD for leachate treatment and 26.94-52.28 CNY/m and 0.71-3.61 CNY/kgTDS for FGD wastewater treatment. The major components of operating costs were chemical costs (13.09 CNY/m, 1.63 CNY/kgCOD) for two-stage DTRO and electricity costs (19.73 CNY/m, 1.67 CNY/kgCOD) for the "biological treatment + DTRO/two-stage DTRO" process. DTRO has shown promising prospects for wastewater treatment.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148291DOI Listing
October 2021

Selenium deficiency causes apoptosis through endoplasmic reticulum stress in swine small intestine.

Biofactors 2021 Jun 15. Epub 2021 Jun 15.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

Selenium (Se) plays a crucial role in intestinal health. However, the specific mechanism by which deficiency of Se causes intestinal damage remains unclear. This study was to explore whether Se deficiency can cause ER stress and induce apoptosis in swine small intestine. We established the Se deficiency swine model in vivo and the intestinal epithelial (IPEC-J2) cell Se deficiency model in vitro. The results of morphological observation showed that Se deficiency caused structural damage in intestinal villi and the decrease of goblet cell structure. The apoptotic characteristics such as nucleolar condensation, mitochondrial swelling, and apoptotic bodies were observed in the IPEC-J2 cells. The results of acridine orange/ethidium bromide and mitochondrial membrane potential fluorescence staining in vitro showed that there were more apoptotic cells in the Se-deficiency group than that in the control group. The protein and/or mRNA expression levels of Bax, Bcl-2, caspase 3, caspase 8, caspase 9, cytc, PERK, ATF6, IRE, XBP1, CHOP, GRP78, which are related to ER stress-apoptosis pathway, were significantly increased in the Se-deficient group which compared with the control group in vivo and in vitro were consistent. These results indicated that Se deficiency induced ER stress and increased the apoptosis in swine small intestine and IPEC-J2 cells and then caused the damage in swine small intestinal tissue. Besides, the results of gene expressions in our experiment proved that ER stress induced by Se deficiency promoted apoptosis. These results filled the blank in the mechanism of Se deficiency-induced intestinal injury in swine.
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http://dx.doi.org/10.1002/biof.1762DOI Listing
June 2021

Giant retroperitoneal myxoma: A case report and literature review.

Int J Surg Case Rep 2021 Jul 29;84:106055. Epub 2021 May 29.

Department of Radiology,The Affiliated Hospital of Guizhou Medical university, Guiyang, China.

Introduction And Importance: Myxoma is a benign tumor and is mesenchymal in origin. Myxomas of the retroperitoneum are extremely rare entities.

Case Presentation: We here report a case of a 67-year-old male who presented with progressive abdominal distention for 3 years. Laboratory investigations revealed a reduction in erythrocytes, lymphocytes, hemoglobin, and an elevation in carbohydrate antigen 19-9. Imaging findings showed a multilocular cystic mass in the right abdomen with thin septa and internal calcifications. Laparotomy revealed that the mass had arisen from the retroperitoneum and the histological study suggested the diagnosis of myxoma.

Discussion: Myxoma features as a "cystic mass" in imaging studies. Therefore, the possibility of a cystic lymphangioma, cystic mesothelioma and myxoma should be considered when a multicystic lesion in the retroperitoneal space is observed. Due to the rarity of retroperitoneal myxomas and lack of specific manifestations and diagnostic methods, preoperative diagnosis is often delayed or incorrect. And until now, only a few cases of retroperitoneal myxoma have been reported.

Conclusion: The report will increase the understanding of the diagnosis and treatment of retroperitoneal myxomas. A brief review of the related literature was also carried out.
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http://dx.doi.org/10.1016/j.ijscr.2021.106055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209073PMC
July 2021

Ultralow-power flexible transparent carbon nanotube synaptic transistors for emotional memory.

Nanoscale 2021 Jul;13(26):11360-11369

School of Electronic and Computer Engineering, Peking University, Shenzhen 518055, China.

Emulating the biological behavior of the human brain with artificial neuromorphic devices is essential for the future development of human-machine interactive systems, bionic sensing systems and intelligent robotic systems. In this paper, artificial flexible transparent carbon nanotube synaptic transistors (F-CNT-STs) with signal transmission and emotional learning functions are realized by adopting the poly(vinyl alcohol) (PVA)/SiO2 proton-conducting electrolyte. Synaptic functions of biological synapses including excitatory and inhibitory behaviors are successfully emulated in the F-CNT-STs. Besides, synaptic plasticity such as spike-duration-dependent plasticity, spike-number-dependent plasticity, spike-amplitude-dependent plasticity, paired-pulse facilitation, short-term plasticity, and long-term plasticity have all been systematically characterized. Moreover, the F-CNT-STs also closely imitate the behavior of human brain learning and emotional memory functions. After 1000 bending cycles at a radius of 3 mm, both the transistor characteristics and the synaptic functions can still be implemented correctly, showing outstanding mechanical capability. The realized F-CNT-STs possess low operating voltage, quick response, and ultra-low power consumption, indicating their high potential to work in low-power biological systems and artificial intelligence systems. The flexible artificial synaptic transistor enables its potential to be generally applicable to various flexible wearable biological and intelligent applications.
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http://dx.doi.org/10.1039/d1nr02099dDOI Listing
July 2021

Pd-Doping-Induced Oxygen Vacancies in One-Dimensional Tungsten Oxide Nanowires for Enhanced Acetone Gas Sensing.

Anal Chem 2021 May 11;93(20):7465-7472. Epub 2021 May 11.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.

Metal oxide semiconductors (MOS) with different nanostructures have been widely used as gas sensing materials due to the tunable interface structures and properties. However, further improvement of the sensing sensitivity and selectivity is still challenging in this area. Constructing appropriate heterogeneous interface structures and oxygen vacancies is one of the important strategies to tune the sensing properties of MOS. In the present study, interfacial heterostructures in PdWO nanowires (PdWO NWs) were fabricated and manipulated by doping different Pd contents through a simple hydrothermal process. Relevant characterization proved that the structure and composition of the one-dimensional (1D) nanomaterial can be effectively changed by Pd doping. It was found that the oxygen vacancy concentration increases first with the increase of Pd content, and when the Pd content increases to 7.18% (PdWO NWs), the oxygen vacancy content reaches the maximum (52.5%). If the Pd content continues to increase, the oxygen vacancy ratio decreases. The gas sensing investigations illustrated that the PdWO NWs exhibited enhanced sensing properties than pure WO NWs toward acetone. Among the as-prepared catalysts, the PdWO NWs showed the best sensing response and the fastest response-recovery speeds (5 and 10 s, respectively) at a working temperature of 175 °C. In addition, this 1D nanostructure with fabricated heterostructures also delivers a good sensing selectivity and a wide detection range from 100 ppb to 300 ppm, with maintaining excellent performance in the presence of high concentrations of ethanol and carbon dioxide. The excellent gas sensing behavior could be attributed to the generated oxygen vacancies and the heterostructures upon Pd doping. This study offers a novel strategy for the design of high-performance gas sensors for ppb-level acetone sensing.
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http://dx.doi.org/10.1021/acs.analchem.1c00568DOI Listing
May 2021

The genome of Nautilus pompilius illuminates eye evolution and biomineralization.

Nat Ecol Evol 2021 07 10;5(7):927-938. Epub 2021 May 10.

Key Laboratory of Tropical Marine Bio-resources and Ecology and Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.

Nautilus is the sole surviving externally shelled cephalopod from the Palaeozoic. It is unique within cephalopod genealogy and critical to understanding the evolutionary novelties of cephalopods. Here, we present a complete Nautilus pompilius genome as a fundamental genomic reference on cephalopod innovations, such as the pinhole eye and biomineralization. Nautilus shows a compact, minimalist genome with few encoding genes and slow evolutionary rates in both non-coding and coding regions among known cephalopods. Importantly, multiple genomic innovations including gene losses, independent contraction and expansion of specific gene families and their associated regulatory networks likely moulded the evolution of the nautilus pinhole eye. The conserved molluscan biomineralization toolkit and lineage-specific repetitive low-complexity domains are essential to the construction of the nautilus shell. The nautilus genome constitutes a valuable resource for reconstructing the evolutionary scenarios and genomic innovations that shape the extant cephalopods.
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http://dx.doi.org/10.1038/s41559-021-01448-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257504PMC
July 2021

Identifying the fouling behavior of forward osmosis membranes exposed to different inorganic components with high ionic strength.

Environ Sci Pollut Res Int 2021 Sep 4;28(34):46303-46318. Epub 2021 May 4.

Research Institute of Membrane Separation Technology of Shaanxi Province, Key Laboratory of Membrane Separation of Shaanxi Province, Key Laboratory of Northwest Water Resources, Environmental and Ecology, Ministry of Education, Key Laboratory of Environmental Engineering, Shaanxi Province, School of Environmental & Municipal Engineering, Xi'an University of Architecture and Technology, Yan Ta Road. No.13, Xi'an, 710055, China.

Functionalized multiwalled carbon nanotube (f-MWCNT) mixed matrix forward osmosis (FO) membranes were fabricated by phase inversion, and the mechanism of sodium alginate (SA) membrane fouling in the presence of various inorganic components with high ionic strength was thoroughly investigated. The membrane incorporated with 0.5% f-MWCNTs (M-0.5) exhibited enhanced performance, which was attributed to the hydrophilicity of the modified nanoparticles and their good compatibility with the cellulose acetate (CA) substrate. Moreover, it was found that the initial permeate flux decline rate for all FO membranes investigated followed the order Na + Ca + Mg > Na + Ca > Na + Mg > Na, which was attributed to the particle size of SA macromolecules in the corresponding solutions. However, the gradual change in attenuation was consistent with adhesion force observations made for the SA-fouled FO membrane in the later steady-state stage, and there was little difference among M-0 (without f-MWCNTs), M-0.5, and M-1 (with 1% f-MWCNTs). Furthermore, the SA adsorption layer was most compact in the presence of Ca, and the flux recovery rate (FRR) was the lowest after simple hydraulic cleaning, but the overall FRRs for FO membranes were greater than 85%. This implies that although a decrease in electrostatic repulsion leads to the formation of a compact fouling layer, an increase in hydration repulsion of hydrated salt ions plays a major role in membrane fouling under high ionic strength conditions.
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http://dx.doi.org/10.1007/s11356-021-14170-4DOI Listing
September 2021

Interaction between dietary branched-chain amino acids and genetic risk score on the risk of type 2 diabetes in Chinese.

Genes Nutr 2021 Mar 4;16(1). Epub 2021 Mar 4.

National Key Discipline, Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, 157 Baojian Road, Harbin, 150081, People's Republic of China.

Background And Objectives: Previous studies have found the important gene-diet interactions on type 2 diabetes (T2D) incident but have not followed branched-chain amino acids (BCAAs), even though they have shown heterogeneous effectiveness in diabetes-related factors. So in this study, we aim to investigate whether dietary BCAAs interact with the genetic predisposition in relation to T2D risk and fasting glucose in Chinese adults.

Methods: In a case-control study nested in the Harbin Cohort Study on Diet, Nutrition and Chronic Non-Communicable Diseases, we obtained data for 434 incident T2D cases and 434 controls matched by age and sex. An unweighted genetic risk score (GRS) was calculated for 25 T2D-related single nucleotide polymorphisms by summation of the number of risk alleles for T2D. Multivariate logistic regression models and general linear regression models were used to assess the interaction between dietary BCAAs and GRS on T2D risk and fasting glucose.

Results: Significant interactions were found between GRS and dietary BCAAs on T2D risk and fasting glucose (p for interaction = 0.001 and 0.004, respectively). Comparing with low GRS, the odds ratio of T2D in high GRS were 2.98 (95% CI 1.54-5.76) among those with the highest tertile of total BCAA intake but were non-significant among those with the lowest intake, corresponding to 0.39 (0.12) mmol/L versus - 0.07 (0.10) mmol/L fasting glucose elevation per tertile. Viewed differently, comparing extreme tertiles of dietary BCAAs, the odds ratio (95% CIs) of T2D risk were 0.46 (0.22-0.95), 2.22 (1.15-4.31), and 2.90 (1.54-5.47) (fasting glucose elevation per tertile: - 0.23 (0.10), 0.18 (0.10), and 0.26 (0.13) mmol/L) among participants with low, intermediate, and high genetic risk, respectively.

Conclusions: This study indicated that dietary BCAAs could amplify the genetic association with T2D risk and fasting glucose. Moreover, higher BCAA intake showed positive association with T2D when genetic predisposition was also high but changed to negative when genetic predisposition was low.
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http://dx.doi.org/10.1186/s12263-021-00684-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934387PMC
March 2021

Fecal g. Streptococcus and g. Eubacterium_coprostanoligenes_group combined with sphingosine to modulate the serum dyslipidemia in high-fat diet mice.

Clin Nutr 2021 06 5;40(6):4234-4245. Epub 2021 Feb 5.

Key Discipline, Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin, Heilongjiang Province 150081, PR China. Electronic address:

Background & Aims: Although high-fat diet (HFD) could impact the composition of fecal microbiome and their metabolites, it is still largely unknown which fecal bacteria and metabolites are relatively important in responding to the HFD. This study aimed to identify the crucial fecal bacteria and metabolites in the HFD mice using a microbial-metabolite network, and to investigate the synergistic mediation effect of the crucial fecal bacteria and metabolites on serum dyslipidemia induced by the HFD.

Methods: The 16srDNA sequencing and the ultra-performance liquid chromatography (UPLC/TOF MSMS) platform were performed to characterize the composition and function of fecal microbiome, and metabolites in the HFD. The microbial-metabolite network, correlation and mediation analyses were performed to examine the relationships among fecal microbiome, metabolites, and serum dyslipidemia indicators. Mice models were conducted to evaluate the effect of fecal metabolite on dyslipidemia.

Results: Compared to the control, 32 genera were altered in the HFD, including 26 up-regulated and 6 down-regulated. A total of 42 altered pathways were observed between the control and HFD, and the "Glycosphingolipid biosynthesis" was identified as the most significant pathway (fold change = 0.64; p < 0.001). Meanwhile, 49 fecal metabolites were altered in the HFD, and the fecal microbiome was associated with the fecal metabolism (M2 = 0.776, p = 0.008). Based on the microbial-metabolite network, two major hub genera were screened (HUB1: g. Streptococcus, HUB2: g. Eubacterium_coprostanoligenes_group), and one bacterial metabolite, sphingosine, was found in this study. Further, the HUB2 was positively associated with fecal sphingosine (r = 0.646, p = 0.001), and its downstream metabolic pathway, "Glycosphingolipid biosynthesis" pathway (r = 0.544, p = 0.009). The regulatory relationship between the HUB2 and sphingosine synergistically mediated the effect of HFD on TCHO (33.7%), HDL-C (37.3%), and bodyweight (36.7%). Besides, compared to the HFD, the HFD with sphingosine supplementation had lower bodyweight (35.12 ± 1.23 vs. 39.42 ± 1.25, p < 0.001), TG (0.44 ± 0.08 vs. 0.52 ± 0.05, p = 0.002), TCHO (3.81 ± 0.34 vs. 4.51 ± 0.38, p = 0.002), and LDL-c (0.82 ± 0.09 vs. 0.97 ± 0.15, p = 0.016).

Conclusions: The g. Streptococcus and g. Eubacterium_coprostanoligenes are two hub genera in the fecal micro-ecosystem of the HFD, and the g. Eubacterium_coprostanoligenes mediates the effect of HFD on dyslipidemia through sphingosine. Sphingosine supplementation can improve dyslipidemia induced by HFD.
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http://dx.doi.org/10.1016/j.clnu.2021.01.031DOI Listing
June 2021

NLRP3 inflammasome mediated pyroptosis is involved in cadmium exposure-induced neuroinflammation through the IL-1β/IkB-α-NF-κB-NLRP3 feedback loop in swine.

Toxicology 2021 04 13;453:152720. Epub 2021 Feb 13.

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, PR China. Electronic address:

Cadmium (Cd) chloride, as widely distributed toxic environmental pollutants by using in industry, severely imperils animal and human health. Pyroptosis is a Cas1-dependent pro-inflammatory programmed cell death and involves in various types of diseases. Nevertheless, the mechanism of pyroptosis and Cd-induced neurotoxicity remains obscure. To investigate the specific molecular mechanisms of Cd-induced neurotoxicity, 10 weaned piglets were randomly divided into 2 groups treated with 0 and 20 mg/kg CdCl in the diet for 40 days. The levels of pyroptosis, mitochondrial and inflammation-related genes were validated by qRT-PCR and WB in vivo. Our results revealed that Cd caused cerebral histopathology lesions, inducing cerebral pyroptosis and the mass generation of inflammatory cytokines, as indicated by the increased NLRP3 inflammasome activation (NLRP3, Cas1 and ASC) and the upregulation of inflammation factors IL-2, IL-6, IL-7 and inhibition of IL-10. Subsequently, further research indicated that Cd triggered pyroptosis via activating the TRAF6-IkB-α-NF-κB pathway, which interfered with the phosphorylation and ubiquitination of IkB-α. Furthermore, Cd caused mitochondrial dysfunction and fragmentation by inhibiting the AMPK-PGC-1α-NRF1/2 signaling pathway and reduced the expression of mitochondrial-related regulatory factors OPA1, TFAM and mtDNA, resulting in the increase of NLRP3 inflammasome. Besides, we found eight hub genes (IKK, IKB-α, NLRP3, TRAF6, NF-κB, AMPK, TNFα and PGC-1α), mainly related to the interaction between the NF-κB pathway and NLRP3 inflammasome. Overall, these results demonstrated that Cd could promote the IL-1β/IkB-α-NF-κB-NLRP3 inflammasome activation positive feedback loop to result in neuroinflammation in swine, which provided new insights in understanding Cd-induced toxicity.
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http://dx.doi.org/10.1016/j.tox.2021.152720DOI Listing
April 2021

Genetic Basis of Gluten Aggregation Properties in Wheat ( L.) Dissected by QTL Mapping of GlutoPeak Parameters.

Front Plant Sci 2020 28;11:611605. Epub 2021 Jan 28.

Henan Institute of Crop Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, China.

Bread wheat is one of the most important crops worldwide, supplying approximately one-fifth of the daily protein and the calories for human consumption. Gluten aggregation properties play important roles in determining the processing quality of wheat ( L.) products. Nevertheless, the genetic basis of gluten aggregation properties has not been reported so far. In this study, a recombinant inbred line (RIL) population derived from the cross between Luozhen No. 1 and Zhengyumai 9987 was used to identify quantitative trait loci (QTL) underlying gluten aggregation properties with GlutoPeak parameters. A linkage map was constructed based on 8,518 SNPs genotyped by specific length amplified fragment sequencing (SLAF-seq). A total of 33 additive QTLs on 14 chromosomes were detected by genome-wide composite interval mapping (GCIM), four of which accounted for more than 10% of the phenotypic variation across three environments. Two major QTL clusters were identified on chromosomes 1DS and 1DL. A premature termination of codon (PTC) mutation in the candidate gene () of the QTL cluster on 1DS was detected between Luozhen No. 1 and Zhengyumai 9987, which might be responsible for the difference in gluten aggregation properties between the two varieties. Subsequently, two KASP markers were designed based on SNPs in stringent linkage with the two major QTL clusters. Results of this study provide new insights into the genetic architecture of gluten aggregation properties in wheat, which are helpful for future improvement of the processing quality in wheat breeding.
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http://dx.doi.org/10.3389/fpls.2020.611605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876098PMC
January 2021

Interfacial heterojunction construction by introducing Pd into WO nanowires to promote the visible light-driven photocatalytic degradation of environmental organic pollutants.

J Colloid Interface Sci 2021 May 1;590:518-526. Epub 2021 Feb 1.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; University of Science and Technology of China, Hefei, Anhui 230026, China. Electronic address:

Photocatalytic degradation of organic dyes by sunlight can greatly simplify the catalytic devices and save the cost. The development of photocatalysts for organic pollutants degradation driven by sunlight at room temperature still faces serious challenges. In this work, we developed a novel Pd-WO nanowire photocatalyst for high efficiency, high stability, and sunlight-induced degradation of methylene blue and neutral red. It was found that after Pd introducing, the band gap energy of WO nanowires lowers from 2.77 to 2.44 eV, which can accelerate the electron jump from the valence band to the conduction band, resulting in the efficient separation of electrons and holes. Meanwhile, with Pd introducing to the WO nanowires, interfacial heterojunction with the Schottky barrier is formed, which can reduce the rate of electron-hole recombination. The catalytic results show that the obtained Pd-WO nanowires demonstrate enhanced photodegradation capacity in comparison with WO nanowires. Especially, with Pd-WO nanowires (10 mg) as photocatalyst, 98.4% of methylene blue and 96.1% of neutral red can be decomposed within 40 min under sunlight irradiation. Besides, the photocatalytic degradation pathways of methylene blue and neutral red on the Pd-WO nanowires are also proposed. This study provides a good strategy for the design and preparation of highly efficient 1D heterojunction-based photocatalysts for the degradation of environmental organic pollutants.
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http://dx.doi.org/10.1016/j.jcis.2021.01.067DOI Listing
May 2021

Semi-quantitative assessment optimized the grading of pulmonary aspiration on salivagram in children.

Ann Nucl Med 2021 Mar 8;35(3):321-327. Epub 2021 Feb 8.

Department of Nuclear Medicine, Children's Hospital of Fudan University, No. 399 Wanyuan Road, Minhang District, Shanghai, 201102, People's Republic of China.

Objective: Salivagram is one of the imaging modalities to detect pulmonary aspiration in children. This study aims to optimize the classification of pulmonary aspiration detected by salivagram with a semi-quantitative analytical method.

Methods: This is a retrospective study involving 737 patients (471 males, 266 females; aged 1 month to 8 years; mean age 5.3 months, median age 3.0 months old) with suspected pulmonary aspiration, who had salivagram done between January 2018 and June 2019. Positive cases were divided into 10 groups (Grade 1, R2, L2, R2L2, R3, L3, R3L2, R2L3, R3L3, and 4) according to the scintigraphic findings. Aspiration index was determined as the ratio of the count in the respiratory tract to the total count in the image field of view and compared among different groups using the receiver operating characteristics (ROC) curve analysis.

Results: A total of 180 cases had positive scintigraphic findings of various grades of aspiration (24.4%, 180/737). There is a high consistency among the two independent nuclear medicine physicians involved in the study, in determining both the disease gradings (κ = 0.919;95% CI: 0.915-0.923) and aspiration index (ICC = 0.994;95% CI: 0.993-0.996). There is no significant difference (p > 0.05) in aspiration index among the gradings in "mild" group (grade 1, R2, L2, L2R2), and "moderate" group (grade R3, R3L2, R3L3). After dividing the different grades into "mild", "moderate" and "severe" groups, the aspiration index of "mild" group is 4.40 ± 2.01, that of "moderate" group is 16.43 ± 8.20, and that of "severe" group is 46.94 ± 14.81. Difference in groups was statistically significant (p < 0.0001). In ROC curve analysis, AUC of "mild" and "moderate" groups is 0.970 and that of "moderate" and "severe" groups is 0.943; the cut-off value with highest diagnostic efficiency is 6.75 between "mild" and "moderate" groups and 38.00 between "moderate" and "severe" groups.

Conclusions: We introduced a semi-quantitative analytical method in pulmonary aspiration on salivagram, to optimize and supplement to the current classification of pulmonary aspiration.
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http://dx.doi.org/10.1007/s12149-020-01564-6DOI Listing
March 2021

Relationship between urine specific gravity and the prevalence rate of kidney stone.

Transl Androl Urol 2021 Jan;10(1):184-194

Department of Urology, Affiliated Zhongda Hospital of Southeast University, Nanjing, China.

Background: The purpose of this study was to evaluate the relationship between urine specific gravity (USG) and the prevalence rate of kidney stone.

Methods: We conducted a cross-sectional study of adult participants (≥20 years) of the National Health and Nutrition Examination Survey (NHANES) from 2007 to 2008. The USG was divided into three groups: <1.008, 1.008-1.020 and >1.020. Univariate and multivariate logistic regression analysis was used to determine the effect of USG on the prevalence rate of kidney stone.

Results: A total of 4,791 patients were included in this study, of which 464 (9.7%) reported a history of kidney stone. Univariate logistic regression analysis showed that age, gender, race, hypertension, diabetes, body mass index (BMI), estimated glomerular filtration rate (eGFR), USG and urine creatinine were closely related to the prevalence of kidney stones. After adjusting for known confounding factors, multivariate logistic regression showed that the prevalence rate of kidney stone increased with the increase of USG (1.008-1.020 <1.008, OR =1.31, 95% CI, 0.09-1.91, P=0.155; >1.020 <1.008, OR =1.71, 95% CI, 1.16-2.54, P=0.007).

Conclusions: The increase of USG was significantly correlated with self-reported kidney stone. This finding helps to identify risk factors for kidney stones as early as possible in the United States.
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http://dx.doi.org/10.21037/tau-20-929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844516PMC
January 2021

Neutrophil-lymphocyte ratio acts as a novel diagnostic biomarker for kidney stone prevalence and number of stones passed.

Transl Androl Urol 2021 Jan;10(1):77-86

Department of Urology, Affiliated Zhongda Hospital of Southeast University, Nanjing, China.

Background: This study evaluated the relationship between inflammatory biomarkers and the prevalence of kidney stones and number of stones passed.

Methods: We conducted a cross-sectional study of adult participants (≥20 years) in the National Health and Nutrition Examination Survey (NHANES) from 2007 to 2014. We calculated key inflammatory biomarkers, such as the systemic immune-inflammation index (SII), neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR) and monocyte-lymphocyte ratio (MLR). Multivariate logistic regression analysis was used to determine the effect of inflammatory biomarkers on the prevalence of kidney stones and number of stones passed.

Results: A total of 21,106 participants were included in the final study, and 1,864 patients reported a history of kidney stones (including 292 who had passed no stones, 1,462 who had passed stones 1-5 times, and 110 who had passed stones >5 times). The chi-square test showed that the NLR, MLR and SII were closely related to the occurrence of kidney stones and the number of stones passed. Multivariate logistic regression analysis showed that a high NLR (>1.72) was associated with an increased prevalence of kidney stones and number of stones passed (OR =1.18, 95% CI: 1.03-1.36, P=0.019).

Conclusions: A convenient biomarker, the NLR can be used as a good predictor for the diagnosis of kidney stones and number of stones passed; these findings are worthy of further research and application in clinical practice.
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http://dx.doi.org/10.21037/tau-20-890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844488PMC
January 2021

MnO anchored polypyrrole nanotubes as an efficient sulfur host for high performance lithium-sulfur batteries.

J Colloid Interface Sci 2021 May 7;589:208-216. Epub 2021 Jan 7.

School of Iron and Steel, Soochow University, Suzhou 215000, China. Electronic address:

Lithium-sulfur batteries have attracted numerous attentions owing to their high theory discharge specific capacity and energy density. However, sulfur cathode usually suffers from poor cycle stability and slow reaction kinetics, caused by its poor conductivity, excessive volume changes during charge/discharge processes, complex sulfur species conversion reaction and the dissolution of polysulfide intermediates. Here, we present a free-standing framework of MnO nanoparticles combine with polypyrrole (PPy) nanotubes as host materials for lithium-sulfur batteries to overcome these issues. In this construction, PPy nanotubes serve as the excellent container of sulfur and physical barrier for the excessive volume expansion of sulfur during electrochemical reaction processes, and the nanotubes also provide an efficient conductive network for the rapid transmission of electrons and ions, while MnO nanoparticles facilitate trapping lithium polysulfides. The coordination of PPy nanotubes and MnO effectively alleviate the shuttle effect as well as enhance the utilization of sulfur. The obtained [email protected] sample shows high capacities of 1419.9 and 925.5 mAh g at 0.1 C and 1 C rate, respectively, and exhibits a low capacity fading rate of 0.062% per cycle for 800 cycles at 1 C rate. This work provides a new and effective way for the design of lithium-sulfur batteries with both high rate performance and long cycle stability.
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http://dx.doi.org/10.1016/j.jcis.2021.01.006DOI Listing
May 2021

Self-powered droplet manipulation system for microfluidics based on triboelectric nanogenerator harvesting rotary energy.

Lab Chip 2021 01 13;21(2):284-295. Epub 2021 Jan 13.

School of Physics and Materials Science, Energy Materials and Devices Key Lab of Anhui Province for Photoelectric Conversion, Anhui University, Hefei, 230601, P. R. China.

Microfluidic technology, as a method for manipulating tiny fluids, has the advantages of low sample consumption, fast reaction, and no cross-contamination. In a microfluidic system, accurate manipulation of droplets is a crucial technology that has been widely investigated. In this work, a self-powered droplet manipulation system (SDMS) is proposed to realize various droplet operations, including moving, splitting, merging, mixing, transporting chemicals and reacting. The SDMS is mainly composed of a triboelectric nanogenerator (TENG), an electric brush, and a microfluidic device. The TENG serves as a high-voltage source to power the system. Using different electric brushes and microfluidic devices, different manipulations of droplets can be achieved. Moreover, by experiments and simulations, the influence of the electrode width, the electrode gap and the central angle of one electrode on the performance of SDMS is analyzed in detail. Firstly, by using electrowetting-on-dielectric (EWOD) technology, SDMS can accurately control droplets for long-distance linear movement and simultaneously control multiple droplets to move in a circular electrode track consisting of 40 electrodes. SDMS can also manipulate two droplets of different components to merge and react. In addition, using dielectrophoresis (DEP) technology, SDMS can separate droplets with maximum volumes of 400 μL and reduce the time of the complete mixing of two droplets with different components by 6.3 times compared with the passive mixing method. Finally, the demonstration shows that a droplet can be manipulated by hand power for chemical delivery and chemical reactions on a circular electrode track without an external power source, which proves the applicability of SDMS as an open-surface microfluidic device. Therefore, the self-powered droplet manipulation system proposed in this work may have great application in the fields of drug delivery, micro chemical reactions, and biological microanalysis.
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http://dx.doi.org/10.1039/d0lc00994fDOI Listing
January 2021

Electrospinning for healthcare: recent advancements.

J Mater Chem B 2021 01 24;9(4):939-951. Epub 2020 Dec 24.

UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UK.

Electrospinning is a simple route to generate polymer-based fibres with diameters on the nano- to micron-scale. It has been very widely explored in biomedical science for applications including drug delivery systems, diagnostic imaging, theranostics, and tissue engineering. This extensive literature reveals that a diverse range of functional components including small molecule drugs, biologics, and nanoparticles can be incorporated into electrospun fibres, and it is possible to prepare materials with complex compartmentalised architectures. This perspective article briefly introduces the electrospinning technique before considering its potential applications in biomedicine. Particular attention is paid to the translation of electrospinning to the clinic, including the need to produce materials at large scale and the requirement to do so under Good Manufacturing Practice conditions. We finish with a summary of the key current challenges and future perspectives.
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http://dx.doi.org/10.1039/d0tb02124eDOI Listing
January 2021

Genetic Polymorphisms of and Lung Cancer Risk in Chinese Northeast Population: A Case-Control Study and Meta-Analysis.

DNA Cell Biol 2021 Jan 23;40(1):132-144. Epub 2020 Nov 23.

Department of Epidemiology, School of Public Health, China Medical University, Shenyang, P.R. China.

Long noncoding RNAs (lncRNAs) play vital roles in development and progression of various cancers. To investigate the relationship between three tag single-nucleotide polymorphisms (SNPs) (rs13252298, rs1016343, and rs1456315) in lncRNA prostate cancer-associated noncoding RNA 1 () and lung cancer (LC) risk, we conducted this study. First, we performed a case-control study, including 576 LC patients and 612 cancer-free controls. Second, a meta-analysis was used to evaluate the association of selected SNPs with risk of overall cancer. We found that rs13252298 and rs1456315 were strongly correlated with risk of LC, nonsmall cell lung cancer (NSCLC), and lung adenocarcinoma. For rs13252298, individuals carrying GG genotype had increased risks of LC compared with those carrying AA genotype (adjusted odds ratio [OR] = 1.565, 95% CI = 1.091-2.245,  = 0.015). A significant result was also found in recessive model with adjusted OR of 1.719. Individuals with GG genotype of rs1456315 were at increased risks of LC compared with those carrying AA genotype. Similar results were found in NSCLC patients. Meta-analysis showed that rs1016343 and rs13252298 were associated with overall cancer. But for rs1016343, no significant association was observed in Asians. In conclusion, rs13252298 and rs1456315 in may be genetic susceptibility factors for LC in Chinese population. These results need to be confirmed by further studies.
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http://dx.doi.org/10.1089/dna.2020.6194DOI Listing
January 2021

Long Non-coding RNA HOTAIR Function as a Competing Endogenous RNA for miR-149-5p to Promote the Cell Growth, Migration, and Invasion in Non-small Cell Lung Cancer.

Front Oncol 2020 25;10:528520. Epub 2020 Sep 25.

Department of Epidemiology, School of Public Health, China Medical University, Shenyang, China.

Lung cancer is a leading cause of cancer death all around the world. Long non-coding RNAs (lncRNAs) have been confirmed to be involved in carcinogenesis of malignancies. However, the molecular mechanism of most lncRNAs in various kinds of cancers remains unclear. LncRNA HOTAIR and HNRNPA1 are reported to play an oncogenic role in non-small cell lung cancer, and the overexpression of HNRNPA1 is shown to promote the proliferation of lung adenocarcinoma cells. In our study, we find that the overexpression of HOTAIR could promote the proliferation and overexpression of miR-149-5p could inhibit the proliferation of lung cancer cells. Flow cytometric analysis determines that overexpression of miR-149-5p induces cell cycle arrest in the G0/G1 phases, whereas overexpression of HOTAIR decreases the proportion of G0/G1phase cells. Also, overexpression of HOTAIR promotes the migration and invasion ability of lung cancer cells, confirmed by the wound-healing and transwell assays, which are suppressed by overexpression of miR-149-5p. Furthermore, the dual-luciferase reporter assay indicates that miR-149-5p could bind both HOTAIR and the 3'UTR of HNRNPA1. In summary, we find that HOTAIR can regulate HNRNPA1 expression through a ceRNA mechanism by sequester miR-149-5p, which post-transcriptionally targets HNRNPA1, thus promoting lung cancer progression.
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http://dx.doi.org/10.3389/fonc.2020.528520DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545358PMC
September 2020

Polyfluoroalkyl chemicals and the risk of kidney stones in US adults: A population-based study.

Ecotoxicol Environ Saf 2021 Jan 19;208:111497. Epub 2020 Oct 19.

Department of Urology, Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, China. Electronic address:

The potential nephrotoxicity of polyfluoroalkyl chemicals (PFCs) have received extensive attention. However, the relationship between PFCs and the risk of kidney stones remain unclear. This study aimed to examine the level of PFCs in the US population and its relationship with the risk of kidney stones. We investigated the serum levels of six PFCs in 8453 adult participants (≥20 years) from the National Health and Nutrition Examination Survey (NHANES) between 2007 and 2016, including perfluorodecanoic acid (PFDE), perfluorohexane sulfonic acid (PFHS), 2-(N-methyl-perfluorooctane sulfonamido) acetate (MPAH), perfluorononanoic acid (PFNA), perfluoroundecanoic acid (PFUA), and perfluorododecanoic acid (PFDO). Logistic regression model was used to evaluate the correlation between PFCs and kidney stones. Of the 8453 participants, 787 self-reported a history of kidney stones. After adjusting for gender, age, race, education, marital status, body mass index (BMI), hypertension, diabetes and estimated glomerular filtration rate (eGFR), we found that total PFCs and PFHS were positively correlated with the risk of kidney stones. Compared with the lowest tertile, the odds ratios with 95% confidence intervals (CI) with increasing tertiles were 1.30 (95% CI,1.08-1.59, p = 0.007) and 1.25 (95 CI%,1.00-1.52, p = 0.024) for total PFCs and 1.24 (95 CI%,1.03-1.51, p = 0.032), and 1.35 (95 CI,1.10-1.68, p = 0.005) for PFHS. Our study shows that total PFCs and PFHS were associated with an increased risk of kidney stones.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111497DOI Listing
January 2021

Selenoprotein Gpx3 knockdown induces myocardial damage through Ca leaks in chickens.

Metallomics 2020 11 24;12(11):1713-1728. Epub 2020 Sep 24.

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China.

Glutathione peroxidase 3 (Gpx3) is a pivotal selenoprotein that acts as an antioxidant. However, the role of Gpx3 in maintaining the normal metabolism of cardiomyocytes remains to be elucidated in more detail. Herein, we employed a model of Gpx3 interference in chicken embryos in vivo and Gpx3 knockdown chicken cardiomyocytes in vitro. Real-time PCR, western blotting and fluorescent staining were performed to detect reactive oxygen species (ROS), the calcium (Ca) concentration, endoplasmic reticulum (ER) stress, myocardial contraction, inflammation and heat shock proteins (HSPs). Our results revealed that Gpx3 suppression increased the level of ROS, which induced Ca leakage in the cytoplasm by blocking the expression of Ca channels. The imbalance of Ca homeostasis triggered ER stress and blocked myocardial contraction. Furthermore, we found that Ca imbalance in the cytoplasm induced severe inflammation, and HSPs might play a protective role throughout these processes. In conclusion, Gpx3 suppression induces myocardial damage through the activation of Ca-dependent ER stress.
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http://dx.doi.org/10.1039/d0mt00027bDOI Listing
November 2020

Effects of ethyl acetate extract of on brain-gut peptides and interstitial cells of gastric Cajal in rats with diabetic gastroparesis.

Iran J Basic Med Sci 2020 Sep;23(9):1218-1224

Department of Pharmacy, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, Shandong, China.

Objectives: Effects of ethyl acetate extract of (EES) on brain-gut peptides and interstitial cells of gastric Cajal in rats with diabetic gastroparesis were explored.

Materials And Methods: Rats were divided into six groups: normal control group (NC), diabetic gastroparesis model group (DGP), low, medium, and high dose of EES groups (LES, MES, and HES, respectively), and metoclopramide positive group (MPG). DGP rats were induced by streptozotocin (STZ) combined with a high-sugar-high-fat diet. The gastric emptying was measured by the phenol red labeling method. Enzyme-linked immunosorbent assay (ELISA) was employed to determine the concentrations of serum ghrelin, gastrin (GAS), somatostatin (SS), and vasoactive intestinal peptide (VIP). The expressions of c-Kit and its natural ligand stem cell factor (SCF) in gastric tissues were determined by Western blot and immunofluorescence.

Results: Gastric emptying rate increased in a different degree after intervention by EES, among which MES and HES groups showed a significant effect (compared with DGP, <0.01) and the HES group was equivalent to the MPG group; serum ghrelin and content of serum GAS increased while SS and VIP decreased (compared with the DGP group, <0.05 or <0.01); c-Kit and SCF protein expressions in gastric tissue increased (compared with DGP group, <0.05 or <0.01).

Conclusion: EES significantly improved gastric emptying by regulating gastrointestinal hormone excretion and c-Kit/SCF signaling pathway. Our study provides a pharmacological basis for the use of the EES in the treatment of DGP. However, the detailed molecular mechanism remains to be clarified.
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http://dx.doi.org/10.22038/ijbms.2020.43521.10223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491494PMC
September 2020
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